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Showing: 341 - 360 of 635 results

  1. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    Published
    Feb. 18, 2015, 3:09 p.m.
    ...characteristics and ectopic reporter assays. We have been interested in exploring the function of non-coding regulatory...frameshift mutations. However these may require restriction fragment length polymorphism analysis, allele-specific...

  2. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...double-stranded DNA break at the genomic loci of interest and on either side of the cargo (ex: fluorescent...the double-stranded break in the genomic loci of interest. As the universal sgRNA is not predicted to target...

  3. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ...of AAV plasmids will have technical or legal restrictions that make them ineligible for use in this service...consideration will have a banner on their material page. Interested scientists can expand the banner to select their...

  5. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...specifically isolate DNA that is bound by a protein of interest. First, nuclei from tissue or cell culture are...with a primary antibody against the protein of interest. The Protein A fused MNase is then added and Protein...

  7. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ...between recurring meetings, you should share interesting results or ask questions to your collaborators...microscopes and allowed me to investigate my protein of interest in a French clinical cohort, neither of which ...

  8. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    Published
    May 2, 2023, 1 p.m.
    ...conditions without necessarily modifying the gene of interest in any way. For example, if green fluorescent ...situations. Tissue-specific genes – the genes of interest may not be expressed in the tissue/cell line you...

  9. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...added to the N or C terminus of your protein of interest. Many of these tags also have commercially available...makes it amenable to being left on your protein of interest, especially if downstream antibody detection is...

  10. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...activated in response to a ligand binding a GPCR of interest. How does TRUPATH work? The TRUPATH collection...transfected with plasmids expressing the GPCR of interest, a Gα-Rluc8 plasmid, and the appropriate Gγ-GFP2...

  11. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ...discovered? The discovery of GeoCas9 started with an interesting question: why engineer a new Cas9 when you can...CRISPR Methods for Bacterial Genome Engineering   Interested in using CRISPR/Cas9 RNPs? Check out this post...

  12. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...allow you to test the function of Your Gene Of Interest (YGOI) in a controlled environment under various... downstream of the open reading frame (ORF) of interest –  try pRS plasmid shuttle vectors. You may prefer...

  15. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...have in common? You can do quality science on interesting topics Having strong non-science skills is important...mentors, to discuss your strengths, weaknesses, interests and passions is another great way to shed light...

  16. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...getting researchers to the experiments they’re interested in faster by producing, titering, and testing...neuronal activity while studying your phenotype of interest. Optogenetic tools for similarly controlling neuronal...

  17. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    Published
    Jan. 24, 2019, 2:24 p.m.
    ...a graduate student at Kyoto University. He is interested in developing genetic tools for neuronal circuit...professor at Kyushu University. He has a particular interest in the development of sensory systems focusing...

  18. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    Published
    Oct. 20, 2020, 1:15 p.m.
    ... had an alter ego as a mad scientist for show wrestling, founded a nonprofit, learned to play the ukelele...interviews are 1:1 discussions with someone who has interesting experiences to share. The conversation builds...

  20. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ... development was something Stegmeier has been interested in for a long time. “At an early age, I was fascinated...reagent, it’s an annotated reagent.” He’s also interested to see Addgene expand the sharing platform to...
Showing: 341 - 360 of 635 results