We narrowed to 645 results for: rest

...can be applied non-invasively, its use is not restricted to cultured cells but extends to freely moving...plasmids have clear application to anyone with an interest in neuroscience, their use is by no means limited...

...discussed further in this informative post. Interestingly, this technology may make some uses of Site ... Software Engineer Kris Shamloo wrote a very interesting post about how we develop our in house lab management...

...screen for pathways that confer any phenotype of interest, not just drug resistance,” he said. “It could...contacted by investigators all over the world who are interested to use our libraries to do things - sometimes...

...characteristics and ectopic reporter assays. We have been interested in exploring the function of non-coding regulatory...frameshift mutations. However these may require restriction fragment length polymorphism analysis, allele-specific...

...Of course, if you’re interested in using IBEX, you’re probably also very interested in having high-quality...

...of AAV plasmids will have technical or legal restrictions that make them ineligible for use in this service...consideration will have a banner on their material page. Interested scientists can expand the banner to select their...

...double-stranded DNA break at the genomic loci of interest and on either side of the cargo (ex: fluorescent...the double-stranded break in the genomic loci of interest. As the universal sgRNA is not predicted to target...

...added to the N or C terminus of your protein of interest. Many of these tags also have commercially available...makes it amenable to being left on your protein of interest, especially if downstream antibody detection is...

...conditions without necessarily modifying the gene of interest in any way. For example, if green fluorescent ...situations. Tissue-specific genes – the genes of interest may not be expressed in the tissue/cell line you...

...between recurring meetings, you should share interesting results or ask questions to your collaborators...microscopes and allowed me to investigate my protein of interest in a French clinical cohort, neither of which ...

...specifically isolate DNA that is bound by a protein of interest. First, nuclei from tissue or cell culture are...with a primary antibody against the protein of interest. The Protein A fused MNase is then added and Protein...

...activated in response to a ligand binding a GPCR of interest. How does TRUPATH work? The TRUPATH collection...transfected with plasmids expressing the GPCR of interest, a Gα-Rluc8 plasmid, and the appropriate Gγ-GFP2...

...works to meet the individual needs of schools interested in using eScience Labs as part of their curriculum...Learn more about alternative science careers here Interested in creative science education tools? Check out...

...discovered? The discovery of GeoCas9 started with an interesting question: why engineer a new Cas9 when you can...CRISPR Methods for Bacterial Genome Engineering   Interested in using CRISPR/Cas9 RNPs? Check out this post...

...allow you to test the function of Your Gene Of Interest (YGOI) in a controlled environment under various... downstream of the open reading frame (ORF) of interest –  try pRS plasmid shuttle vectors. You may prefer...

...with the viral plasmid containing the cargo of interest, and HEKs will produce and package viral particles...be a great option! Especially if your research interests include receptor biology, ion channels, or involve...

...mice, by replacing the effector with a gene of interest in the cells you are studying. Read our SPARC ...single molecule-fluorescence assay to monitor restriction enzyme digestion of single molecules in real-...

... development was something Stegmeier has been interested in for a long time. “At an early age, I was fascinated...reagent, it’s an annotated reagent.” He’s also interested to see Addgene expand the sharing platform to...

...downstream applications (Berns, 1972). Using qPCR and restriction enzyme based methods, we showed that the DNA ...easily customized to detect other sequences of interest.   While the VGS process isn’t new to Addgene,...

...inducible CMV promoter and targeted to a sequence of interest with an sgRNA expressed from a separate plasmid...dCas9–APEX2 biotinylation at genomic elements by restricted spatial tagging), to confirm the composition ...