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...If your lab owns a liquid handling robot, you can set it up to automate the reagent transfer and the restriction–ligation...

...it’s a combination of many factors unique to this set of tools and the community they serve. As difficult...validating and using an AAV in a specific experimental setup. Teams across Addgene have worked hard to ensure...compared to other AAVs preps from 2016–2023. B) Inset showing distribution of only GCaMP6, jGCaMP7, and...

...in the best-case scenario [7]. In the clinical setting where patient blood samples are limited, such low...by Kusumika (Kushi) Mukherjee, a Postdoc at Massachussetts General Hospital. A little over a decade back...Mukherjee is currently a postdoctoral fellow at Massachusetts General Hospital with research interests in ...

...frogs, mice, and even humans. It binds to a specific set of DNA-binding transcription factors to repress the...

...Tempurature: Analyze your expression conditions by setting up a small-scale expression experiment to test ...StrR, TetR) Expression of insoluble proteins  Rosetta2 (DE3)* Chloramphenicol (pRARE) Good for “universal... plasmid contains a chloramphenicol resistance cassette for positive selection and a p15A origin of replication...

...co-factor, (iii) easy visualization with standard filter sets on a fluorescence microscope, and finally (iv) good...

...with potentially harmful mutations in therapeutic settings. Silencing of a target gene is achieved through...; font-weight: normal; text-decoration: none","font_set":"DEFAULT"},"button_hover_color":{"color":"#0052a9...

...mutagenesis is especially concerning in clinical settings, as evidenced by retroviral gene therapy trials...

...PMCID: PMC3748948.  Khetrapal, Varnica, et al. "A set of powerful negative selection systems for unmodified...expression of a gene with an antibiotic resistance cassette. This leaves a “scar” in the chromosome, sometimes...vector contains sacB, an chloramphenicol resistance cassette, and the R6K origin of replication to allow replication...pDS132 (Philippe et al., 2004): Replaced the sacB cassette with one of three toxins from the Khetrapal paper...medicine and clinical infectious diseases at the Massachusetts General Hospital where he is now an attending...

... uses Cre to trigger the inversion of two tandem sets of genes aligned head-to-head and the deletion of... same XFP transgene in an adult fly. As a stop cassette is located directly upstream of the fluorescent...

...range. With this in mind, Ed Boyden and colleagues set out to engineer a potassium ion biosensor with a ...

... this skill that you could strengthen. The right set of transferable skills, coupled with your technical...know you are bringing this asset to your job, you can become a valuable asset yourself.     Fig... point of view and can’t understand why they’re upset, conflict resolution is likely not one of your strengths...

...bidirectional USA-Europe professional collaborations, setting up bases and contacts with Spanish laboratories...Universities in USA and Europe (Harvard University, Massachusetts Institute of Technology, Boston University, ...

...use, and robust in performance. In its natural setting, CRISPR-Cas9 is an acquired immune system in bacteria...Yamaguchi T, Nakauchi H (2018) Intra-embryo Gene Cassette Knockin by CRISPR/Cas9-Mediated Genome Editing...

...each individual target is stained correctly Image a set of samples where you label only one target in each...

...responsibility, repeated reminders of blunders, setting target up to fail, shifting goal posts without ...has no idea who is dating whom Expanding your skillset Scientists are lifelong learners. I believe we...

...RNA expression of your gene of interest across a set of human tissues.  From this data, you see that there...and (2) large-scale proteomic and transcriptomic datasets already exist.   Example: You want to use an antibody...

...antibody is used to visualize the proteins. In this set-up, multiple secondary antibody molecules bind the...another great option to enhance resolution. In this setup, gels have an increasing range of acrylamide content...

...large pan-cancer CRISPR-Cas9 gene dependency data sets. Nat Commun 10: . https://doi.org/10.1038/s41467..._style":true,"button_font":{"color":"#FFFFFF","font_set":"DEFAULT","size_unit":"px","font":"helvetica"...nuclease-mediated off-target effects.” Further, large-scale datasets of hundreds of genetic screens using genome-wide...

...variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting that researchers...