...mutagenesis is especially concerning in clinical settings, as evidenced by retroviral gene therapy trials...

...bidirectional USA-Europe professional collaborations, setting up bases and contacts with Spanish laboratories...Universities in USA and Europe (Harvard University, Massachusetts Institute of Technology, Boston University, ...

...responsibility, repeated reminders of blunders, setting target up to fail, shifting goal posts without ...has no idea who is dating whom Expanding your skillset Scientists are lifelong learners. I believe we...

...RNA expression of your gene of interest across a set of human tissues.  From this data, you see that there...and (2) large-scale proteomic and transcriptomic datasets already exist.   Example: You want to use an antibody...

...antibody is used to visualize the proteins. In this set-up, multiple secondary antibody molecules bind the...another great option to enhance resolution. In this setup, gels have an increasing range of acrylamide content...

...large pan-cancer CRISPR-Cas9 gene dependency data sets. Nat Commun 10: . https://doi.org/10.1038/s41467...nuclease-mediated off-target effects.” Further, large-scale datasets of hundreds of genetic screens using genome-wide...

...each individual target is stained correctly Image a set of samples where you label only one target in each...

...use, and robust in performance. In its natural setting, CRISPR-Cas9 is an acquired immune system in bacteria...Yamaguchi T, Nakauchi H (2018) Intra-embryo Gene Cassette Knockin by CRISPR/Cas9-Mediated Genome Editing...

...variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting that researchers...

...is orthogonal to their scientific research skill set. You'll need to motivate your trainees, communicate...

... discovery of RNA guided transposons in bacteria sets the stage for combining the super powers of CRISPR... transposons encode reporter genes, mutagenic cassettes, or barcodes. When transposons are delivered to...

... done things that I never even imagined before I set foot in the lab. They’re also more than eager to ...

...initiates different immune cascades. In the research setting, antibodies of different isotypes can be used together...of antibodies is used to both identify the cell subsets of interest from among the mixture and to measure...

...different-sized multimers, you may even see different sets of bands on the gel corresponding to each type of...

...its expression, and is flanked (“floxed”) by two sets of incompatible recognition sites (e.g., loxP and...

...anise?” This is something I was expecting when I set out to make my first CRISPR/Cas9 gene edit. I wanted...

...March for Science in Albany, NY, many tables were set up with various science demonstrations, and students...

... Mello labs also identified several experimental set ups that could improve CRISPR efficiency in C. elegans...introduced. Cre-mediated excision of the selective cassette minimizes the sequence added, leaving a 34 bp ..., however the development of the self-excising cassette (Dickinson et al., 2015) made this step as simple...Engineering with a Self-Excising Drug Selection Cassette. Genetics 200:1035–1049 . https://doi.org/10.1534...genetics.120.303564 Kim H, Ishidate T, Ghanta KS, Seth M, Conte D Jr, Shirayama M, Mello CC (2014) A Co-CRISPR...

... easier to automate and use in high-throughput settings, but when it comes to low-throughput applications...recommend avoiding a High Quantity/Low Quality mindset for any applications. DNA quantity is fairly self...reactions with relatively little work after the initial setup. (Addgene actually uses some of these — if you’re...

...microscopist at your institution when heading into a new set of experiments. Many thanks to our guest blogger...number of optical and chemical ‘tricks’ to turn subsets of fluorescent molecules on and off. This can provide...