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Addgene

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We narrowed to 396 results for: organization Addgene

Showing: 181 - 190 of 396 results

  1. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ...for Scientists Resources on Addgene.org Learn about Careers at Addgene ...accordingly. Read Additional Career Posts on the Addgene Blog How did this change in labor laws come about... salaries (which have been made by multiple organizations across academia). Increased postdoc wages, overtime... salaries as their website implies. These 5 organizations, according to the NSF data, account for 18% ...Research @FORsymp.   Additional Resources on the Addgene Blog Read about the 2015 and 2016 Future of Research...

  2. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...region (approximately 500bp is best) and select the organism. It will then tell you the available gRNA targets...can also find many previously validated gRNAs at Addgene. In vitro screening for the best gRNA Figure...Find Plasmids for Testing Your gRNAs in vitro at Addgene CRISPR/Cas9 gene editing in mice Now that you’ve... much more from our favourite mammalian model organisms in the near future.  Many thanks to our guest...PMCID: PMC3969854. Additional Resources on the Addgene Blog Get the Basics of Mouse Genome Engineering...Homology Directed Repair Additional Resources on Addgene.org Find Software Tools to Help Design You gRNA ...

  3. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...and stably maintain the genes they carry among organisms belonging to many different phylogenetic groups...bacteria and express proteins within different host organisms. pBBR1-based plasmids and plasmids in incompatibility...  Find broad host range recombinant vectors at Addgene!   References and resources References Doran, ...10.1007/s13205-023-03507-0  More resources on the Addgene blog Plasmids 101: Origin of Replication Plasmids...

  4. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...Blotting Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...intact cell, tissue, or, in some cases, a whole organism. But, as with any antibody-based technique, validation...you are confident, especially if you work in an organism that is underrepresented in the biomedical research...10.1038/nmeth.3995 Additional Resources on the Addgene blog Learn about research applications of antibodies...

  5. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...over-express coding sequences from a given cell type or organism, have been used in a similar manner to Cas9 activators...lines (HEK293T, MCF7, U2-OS, Hela, N2A, 3T3) and organisms (Chavez et al., 2016). To learn more about the...    Alejandro (Alex) Chavez is a member of the Addgene Scientific Advisory Board. His laboratory at Columbia...doi.org/10.1038/nbt.3149 Additional Resources on the Addgene Blog Learn how to Choose the Best Cas9 Variant... CRISPR Has Been Used with AAV Resources on Addgene.org Find Cas9 Activators Find Cas9 Repressors Catch...

  6. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...plasmids with Addgene. Additional control plasmids for the TKO library will be available at Addgene soon. Hart...cellular compartments (cytosol, Addgene catalog #71245; mitochondria, Addgene catalog #71247) Rosella provides... in vitro cell systems and diverse model organisms. Addgene’s newest TALEN kit, the FusX assembly system...read? Listen to our hot plasmids segment on the Addgene Podcast. View Plasmids from:  March (Q1) | June...sequencing. The STARR-seq backbone is available from Addgene in both human and fly versions. Arnold, et al....alongside a growing number of CRISPR libraries from Addgene’s repository that can be used to perform screens...PMID: 24395366 Oxford Drosophila CRISPR library Addgene has had the privilege of distributing several human...

  8. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    Published
    Jan. 23, 2014, 3:23 p.m.
    ...plasmids you use to bring this into your target organism. We have designed a Golden Gate system based on...The complete GreenGate toolkit, now available at Addgene, also gives the option to stack several expression...motivations for making GreenGate available through Addgene,” Lohmann said. “We strongly believe the free sharing...  Kendall Morgan...

  9. Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton

    Type
    Blog Post
    Published
    July 7, 2017, 1:32 p.m.
    ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Along these lines, Darrell recently joined the Addgene Board of Directors where he hopes to help keep ...of this technique will someday allow us to make organs using iPS cells derived from patients. Chapter ...Chapter 12: Darrell's goals as a member of the Addgene board of directors - increasing open source sharing...Additional Resources from Addgene Learn about Retinal Gene Therapy from Connie Cepko Find Plasmids from the...

  10. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...protocols Sep-OTSλ plasmid - Addgene # 68292 C321.ΔA bacterial strain – Addgene #68306 pCRT7 NT TOPO E17TAG...E17TAG GFP plasmid - Addgene #68295 SupD plasmid – Addgene # 68307 For further technical reference and protocols...amongst a subset of methanogenic archea: these organisms lacked a classic cysteinyl tRNA synthetase, despite...flavors’ of Sep-OTS plasmids are available through Addgene, but we have worked most extensively worked with... recombinant phosphoproteins’ available on the Addgene website. Many thanks to guest blogger Natalie ... Lajoie, Marc J., et al. "Genomically recoded organisms expand biological functions." Science 342.6156...Protoc. doi 10 (2008). Journal. Resources on the Addgene Blog Verify Your Constructs with Colony PCR Read...
Showing: 181 - 190 of 396 results