...can do and, in this way, science has always been related to government and, consequently, politics. More...Inclusion of data informing policy specifically related to the following subjects: Climate change Vaccines...Healthcare Science education To make scientists relatable (17, 20). This is an effort to show the general...all you need to do is select your state. State Legislatures: To find the website to contact representatives...Lawmakers need to use a scientific approach to formulating views Pew Research Center: Public and Scientists...

...strategy for increasing the size of the AAV donor template for CRISPR-mediated homology directed repairs ...acceptor of the transgene to help prevent the accumulation of such truncated proteins. Examples of use...homologous recombination approach delivers large donor templates using two sequential homology directed repairs... the gRNA target site that mediated the donor template’s integration so that the site is reconstituted...which results in the integration of a larger donor template.  Drawbacks: Transgene expression varies with...

...Having successfully navigated the trials and tribulations of the process, I’ve provided guidance and mentoring...interviews but no job offers, is a failure to clearly articulate one's talents and ideas. Some key points from...question? This should be something that anyone could relate to, that people would agree is important to understand...provide the first defined model system that we can manipulate to study pathology and therapies." This is...interview, your previous success will not necessarily translate into getting a job. Your papers and pedigree get...

...factor fusions modulate iPSC reprogramming efficiency. Listen to the segment on modulating iPSC reprogramming...Hot Plasmid 3: Regulating telomere length with TZAP. Listen to the segment on regulating telomere length...

..., generating new therapeutics, and sometimes formulating new biological concepts. However, rates of reproducibility... However, even simple procedures like pouring a plate or running a gel have intricacies that aren’t always...have plans to expand to other protein technologies later. Organizations such as BenchSci and FPbase are working...find information about research tools. BenchSci collates information about antibodies while FPbase curates... soon, the Center for Open Science does host a platform for pre-registration wherein researchers can submit...

...Tetracycline-Dependent Transactivator Correlates with Long-Term Doxycycline-Regulated Transgene Expression in Nonhuman...primate. Likewise, in vitro efficacy doesn’t always translate to in vivo efficacy. You’ll therefore need to ... strong early gene expression, but can become methylated over time, resulting in decreased expression ...consider. First, the titer required is inversely related to transduction efficacy: the higher the efficiency...

... 2,3-N-linked sialic acid4 AAV receptor2 Platelet-derived growth factor receptor (PDGFR) α and β3...and so on, although we often use abbreviated nomenclature (AAV1, AAV2, etc.) to distinguish the capsids...-stock viral prep service.  AAV2 Despite being isolated second, AAV2 is the best studied and most commonly...our in-stock viral prep service.  AAV9 AAV9 has relatively broad tissue tropism and high transduction efficiency...retinal gene delivery from the vitreous. Science Translational Medicine, 5(189), 189ra76. https://doi.org/10.1126...

...Being a scientist in my late 20’s, new graduates often ask me for advice on careers available to new ... felt I needed more practical lab experience to later gain a full time position in a lab. I worked on ...left me optimistic that I would find a genetics related role soon after finishing my MSc. The masters program...I had received all the training and experience relating to good manufacturing practice (GMP) and good ...and decided to compromise and applied anyway. I later found out the job was with Addgene. I had used plasmids...

...broth and on the agar plates. Mycoplasma colonies are easily visible on the agar plates. This method is capable...detection, DNA staining, fluorescent tagging, agar plating, etc. Be wise to choose any of the techniques listed... (Uphoff and Drexler, 2011). Culturing on agar plates/broth is considered the ‘gold standard’ for detecting...prevent contamination rather than eradicating it later. Don’t be scared when mycoplasma infects your cell...

...investigator. In basic science (as opposed to translational, biomedical, or applied sciences), people conduct...everything there is to know is a strange and exciting revelation. Science in any field can generally be applied...neurodegenerative disease because they had close relatives suffering from similar diseases. Others were inspired...t know yet has helped me cultivate stronger relationships with my labmates. Sometimes what I thought was...students, women, BIPOC, and other minoritized populations. Research universities often have scholarships...

...genome-wide CRISPR inhibition Covalent tethering of DNA template to Cas9 SECURE base editors Nme2Cas9 CRISPR interference...inhibition library Covalent tethering of DNA repair template to Cas9 Homology-directed repair (HDR) can be ...using a single-stranded oligodeoxynucleotide HDR template covalently attached to the Cas9-gRNA RNP complex...Candida albicans A new CRISPR interference (CRIPSRi) platform for targeted gene repression in Candida albicans...

...like manipulations, anomaly, and so on, differently than scientists would. For example, “manipulate” could...different topics of science, especially disease related topics. Neurodegenerative and brain disorders are...illicit tampering” to general audiences while “manipulate” could mean “worked with” or “changed” to scientists...

...said. “It opens a new avenue of modifying and manipulating endogenous eukaryotic RNA.” Endless possibilities...Biological Engineering in March is fused to a translational repressor. In principle, however, designer PUF...PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1...(1):7. doi: 10.1186/1754-1611-8-7. PubMed.  Related Resources Addgene's Synthetic Biology Overview More...

...Expanding your network of relationships early and often is the most effective tactic a scientist in training...result of existing professional (and personal) relationships. However, most job seekers will and should apply...after the academic bench. Utilizing existing relationships as part of the job application process can dramatically...you can use alumni databases, friends, and even relatives to network your way to a hiring manager. Learn...

...by an inexpensive and widely available imaging platform: ultrasound. Challenges of in vivo imaging with...water-dwelling photosynthetic bacteria and archaea to regulate their buoyancy. The Shapiro lab recently discovered...ultrasound imaging. Ultrasound is an attractive imaging platform for in vivo imaging experiments because  ultrasound...imaging to show where the bacteria localize in relation to internal organs. The limit of detection for...

... live animal that can be correlated to behavior, the technique is relatively slow and is not suited to...activated and inhibited neurons in live mice Report modulation of DA signaling during learning and reward consumption...Petzold, and Paul Greengard. "Dopamine-sensitive adenylate cyclase in caudate nucleus of rat brain, and its...

...the use of light sensitive proteins (opsins) to manipulate cell activity, enables researchers to silence...neuron more positive or more negative.  Neuronal stimulation: Depolarization When the inside of the neuron...the overlap between groups.  They found that photostimulating the Kolmer-Agduhr cells was sufficient to ...Kolmer-Agduhr cells on one side of the body required photostimulation in order to drive the full symmetrical response...

...opportunities for investigating genetic mechanisms related to morphogenesis, cell fate specification, and ...a conformational change of the receptor which modulates the fluorescence intensity of the cpGFP without...agrochemical compounds that have the potential to regulate the parasitization of cereal crops. As a result...activity on NAG PAMS and reduced activity on NGG PAMs relative to wild type. A new genome-wide CRISPR knockout...

...to detach from the constant stream of thoughts related to lab research and gain fresh perspectives. Such...encourages exploration of tangents or seemingly unrelated ideas. It's beneficial to carry a notebook at ... identify areas in need of improvement. These unrelated ideas might hold the key to unlocking new perspectives...everything is a miracle.” This quote encourages us to contemplate our approach to life and how our viewpoints influence...

...system with your homologous recombination (HR) template, you could then: Confirm your HR by doing Restriction...2013 Science paper). If you detect positive HR, isolate single-cell colonies, grow them up, then perform...screen for positive ones.  -- OR -- If your HR template has a selection marker such as puromycin, you ... Biotech, 2 March 2014. Or browse the articles related to the most frequently requested CRISPR plasmids...