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  1. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...last updated Nov 10, 2020. Now that we know all about antibiotic resistance genes, let’s consider another...the replicon. For example, the pMB1 ori maintains about 20 copies per cell, while pUC – which differs by...

  2. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...Our work accelerates and enhances productive collaborations as well as provides visibility for grant-driven...research is accelerating essential research in laboratories all around the world." We thank the Chan Zuckerberg...

  5. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ... is difficult for new members of the lab or collaborators unfamiliar with these mutant plants. Using RUBY...resistant disease. (RUBY-red: You can’t beet it!) About the Author  Andrew Willoughby is a graduate student...

  6. Antibodies 101: Epitope Availability

    Type
    Blog Post
    Published
    Jan. 11, 2022, 2 p.m.
    ...naturally.  Sample Denaturing  First, let’s think about induced conformation changes. Several types of assays...immunoimaging assays. It can also elucidate information about what a given protein is doing or changing in different...

  7. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ... using biorender.com   If you’re new to talking about viruses, here’s a quick tip on terminology. “Virus...have a growing number of Viral Vectors 101 posts about each type, so be sure to check our blog, or our ...

  8. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...Tags have a lot of major benefits – as discussed above – but there are a few instances when they should... hindrances, and destabilization can be brought about by the tag so it’s important to validate tagged ...

  10. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at the same time...Additional Resources on the Addgene Blog Learn About Other Cloning Techniques Catch Up on All Things ...

  11. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    Published
    March 6, 2014, 7:12 p.m.
    ...getting together and chatting with another person about their career path is a great start to a mentoring...Medical School researcher Connie Cepko to learn about her mentoring style. Set goals with a mentor: The...

  13. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...skills and experience.You might not normally think about it, but hobbies and volunteer experiences, as well...show that you have some of the abilities listed above. Showing that you are willing to exercise these ...

  14. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...conduct the screen. To help alleviate some of this labor for our users, we now provide the Brunello (targeting... hear from you if you have used or are thinking about using our viral services. Please send any questions...

  15. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...brightness after ~100 action potentials. This is about twice as bright as GCaMP7f and GCaMP7s. See all...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

  17. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...vector name and serotype you’d like to be notified about. Controls Plasmid Serotype Name 37825  AAVrg...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

  19. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ... it can be used in a lab? Here we’ll be talking about the ins and outs of this classification system, ...The classification system is also useful in a laboratory setting. When designing a plasmid to be inserted...

  20. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...changes. Using this road map, and what was known about previously characterized TFs, the Zhang group were...Additional Resources on the Addgene Blog Learn More about Lentivirus Figure out How to Get the Most from Your...

  21. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ... who aren’t familiar with it. If you’re curious about the hows and whys of the material transfer agreement...easier for others.  If you’ve still got questions about our MTA process, you can check out our Tech Transfer...

  22. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...Medical School researcher Connie Cepko to learn about her management style. Recipes for delegation success...accountability? What types of issues do you want to know about right away? Provide adequate support. The manager...

  24. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ... single amino acid. Without further information about properties like photostability or aggregation tendency...Fluorescent Proteins for Multicolor Imaging Learn about Fluorescent Protein Biosensors Additional Resources...

  25. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...targets compared to Cas9.  Read on to learn more about Cpf1 multiplexing.  For an in-depth review of Cpf1... Addgene. If you have any questions or thoughts about Cpf1 multiplexing, leave them in the comments below...

  26. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    ...advantages over mismatch nuclease assays that will be elaborated below. Digital PCR Digital PCR (dPCR) involves...type targets. Comparatively, detection limits of about 5% mutant targets have been reported for mismatch...

  27. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...Resources on the Addgene Blog Learn More About Lentivirus Learn more About AAV Get the Scoop on Our Viral Service...

  30. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...vector name and serotype you’d like to be notified about. Chemogenetics Plasmid Serotype Name 44362 ...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...
Showing: 541 - 570 of 824 results