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We narrowed to 792 results for: LAT

Showing: 541 - 570 of 792 results

  1. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...ummmm, what is it? Reddit is a volunteer-organized platform where users can participate in forum discussions...to see the questions first makes Reddit a great platform for those who aren’t so great at coming up with...whole point is to meet actual people to build relationships. I also like Twitter because I can get a little...

  2. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...path, you’ve probably got a gene you’d like to manipulate in mind. Now it’s time to consider where you ...mice once they have been generated (more on that later). Find Validated gRNAs to Target Mouse Genes To ...biology and genetics. She loves to communicate the latest and greatest in scientific content.      References...

  3. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...enough to promote cell growth. Overgrown cultures stimulate quiescence, the resting phase of the cell cycle...that the results obtained in these lines can be translated to other lines. Differences in the physiology...therefore titers obtained in one cell type may not translate well to other cell types. To overcome this issue...

  4. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...publications. The policy was not an overnight revelation, rather it was the result of consultations between...representative or the statistical variation is calculated and placed in the legend. Widely reported difficulties...interfaces where the viewer is more likely to want to manipulate how they view the information. We are used to...

  5. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...the mRNA — the bound miRISC can simply block translation machinery. The composition of the RISC varies...MicroRNA biogenesis, mechanisms of actions, and circulation. Frontiers in Endocrinology, 9. https://doi.org...M. A. (2021). DICER: structure, function, and regulation. Biophysical Reviews, 13(6), 1081–1090. https...

  6. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...multiplexing is that you can ask how multiple proteins relate to one another in the same sample. For this strategy...are known to express your target or have been manipulated to overexpress it. For negative controls, use... The biggest difference between IF and ICC/IHC relates to the type of secondary antibody you use, so as...

  7. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...work does relate, make sure to include that material, but don’t force it. You can always relate to scientists... who will be listening.  Are they early, mid, or late career?  Will many non-scientists be present?  Always...

  8. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    Published
    Nov. 25, 2015, 4 p.m.
    ...speaker Paula Stephan did a fantastic job of articulating many of these problems and some solutions. The...provided as an explanation for why they are paid relatively low wages (Stephan estimates that postdocs are...that PIs no longer save grant money by paying relatively low postdoc salaries as opposed to Staff Scientists...

  9. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...controls and then moving on to controls for antibody-related applications. After reading, you will have a better...well-studied kinase known to prevent tau hyperphosphorylation. You could run brain samples from both groups... molecular weight shifts indicative of tau phosphorylation. By including the positive control, you will...

  10. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...the right decision. If I’d spent my senior year isolating pheromones from various C. elegans mutants by ...course, many types of teams. Some teams may be relatively permanent; some teams may only need to exist ...on the other side of the world, communication platforms like Slack make teamwork easier. Trust Ultimately...

  11. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...extract light from a single, thin plane within a relatively thicker three dimensional sample (see Figure ...technique should I use for my experiment? When I am contemplating which microscope to use for a new sample I always...optical sectioning (5). One such example is the lattice lightsheet that projects a number of thin cone-...

  12. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...sequence came from. Entering this information will populate a list of possible Entrez Gene matches to your...easier for other scientists to find information related to your plasmid. It is optional to include the ...correct Entrez Gene does not appear in the auto-populated list, try entering the gene's Official Full Name...

  13. Introducing the Addgene Antibodies 101 eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2024, 1:15 p.m.
    ... first edition of our Antibodies 101 eBook! The latest addition to our eBook collection is a handy resource...start looking for answers.    We hope you find the latest addition to our eBook collection useful. And, as...

  14. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    Published
    Jan. 31, 2018, 2:44 p.m.
    ...package with Addgene mugs and some delicious hot chocolate for the lab. Here are some of our 2017 contest...Thank you to everyone who participated and congratulations to our 2017 #Deckthelab winners. We hope everyone...

  15. 2021 Deck the Lab Winners!

    Type
    Blog Post
    Published
    Jan. 25, 2022, 2:15 p.m.
    ...festive makeover today✂️❄️🦠thanks to the awesome templates by @CVRHutchinson @CVRinfo. Check them out: https...Mugford (@samtmugford) December 3, 2021   Congratulations to all our winners, and a huge thank you to...

  16. New Videos: Addgene Lab Tips

    Type
    Blog Post
    Published
    Feb. 15, 2018, 2:41 p.m.
    ...automated vial filling, accurate tube-labeling, quick plate pouring, and much more. We didn’t want to keep the...Tip” filter on the blog to keep up to date on the latest episode.  Have some suggestions for future tips...

  17. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    Published
    Oct. 17, 2024, 1:15 p.m.
    ...repository what it is. So we’re thrilled this week to congratulate depositor David Baker, who was awarded the Nobel...developed with their protein design approach. Congratulations to David Baker, Demis Hassabis, and John Jumper...

  19. Our New Antibody Mascot is....

    Type
    Blog Post
    Published
    Feb. 24, 2022, 2:15 p.m.
    ...help us prepare for our upcoming antibody launch in late March!      *If you submitted the names Abi, Lin...New Antibody Friend We need your help naming our latest mascot, an antibody joining us on our scientific...

  20. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...be used to cut the DNA sequence. With a repair template, CRISPR can be used to introduce a functional ...CRISPR nuclease target multiple genes in a cell population. The resulting mutant cells are screened for ...

  21. Depositor Week 2020 is Happening Now. Join us!

    Type
    Blog Post
    Published
    Nov. 16, 2020, 2:15 p.m.
    ... that we can offer the scientific community the latest and greatest plasmid tools! Thank you depositors...globe very quickly. There are over 2,300 COVID-19 related plasmids currently in Addgene’s repository which...

  22. New Viral Vectors - Spring 2025

    Type
    Blog Post
    Published
    April 29, 2025, 1:30 p.m.
    ...serotype, and a dTomato under the control of the E6 regulatory element, which targets it to VIP cortical interneurons...forget to use the link below to keep up with the latest in Addgene's viral vector services and resources...

  23. New Viral Vectors - March 2024

    Type
    Blog Post
    Published
    March 5, 2024, 2:15 p.m.
    ...only March!)  In our repository, you'll find the latest iteration of GCaMP, GCaMP8, available in new serotypes...forgot to use the link below to keep up with the latest in Addgene's viral vectors services and resources...

  24. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    Published
    Sept. 25, 2018, 2:39 p.m.
    ... out in time - from assembling the boxes, to inoculating tubes, and to packing and shipping. Every single... This massive scale reagent sharing led us to calculate how much time we have saved researchers. In a ...

  25. Plasmid Grant to Aid Cervical Cancer Screening in Ghana

    Type
    Blog Post
    Published
    Nov. 8, 2016, 3:30 p.m.
    ...Cervical Cancer is the leading cause of cancer related morbidity and death among females in Ghana and ...for cervical cancer development and also better correlate the presence of certain HPV subtypes with risk...

  26. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    Published
    Sept. 1, 2016, 2:30 p.m.
    ... should talk to if ruminating the trials and tribulations of the modern PhD program. Don’t be fooled! ...Medical School. He studies developmental gene regulation with Dr. Angela DePace in the Department of Systems...

  27. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    Published
    Feb. 13, 2014, 1 p.m.
    ...the order they appear in the plasmid and their relative position to the insert. Example, if you have a... immediately followed by its position (number) relative to the start Methionine (Met) followed by the ...

  28. Bringing Sustainable Practices to the Lab: Easy Action Items

    Type
    Blog Post
    Published
    April 22, 2019, 12:26 p.m.
    ...Join a newsletter to stay up-to-date on all the latest news and encourage your peers to do the same. ...reagents stable for years. In fact, some scientists speculate the -80 °C setting might have been a marketing...

  30. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ... genes in clonal populations and to knock-out thousands of genes in mixed population of cells as part ...
Showing: 541 - 570 of 792 results