...validated in.  If a plasmid's Addgene ID # has been referenced in other publications, you'll find a link to ...Additional features Once you've clicked on the "# References" link under the "Resource Information" heading...Addgene’s Plasmids 101 eBook Protocols and Plasmid References @Addgene  ...

...splicing, imaging, and more. Abil says she is currently working on a version that she hopes will change...PUF proteins lose their solubility. Her lab is currently investigating the issue and hopes to have a better...happy to share updated versions of the kit.” Reference Abil Z, Denard CA, Zhao H.  Modular assembly of...

...colleagues you help will be future advocates and references. Collaborate with another lab, in another field... a committee and do something big like plan a conference Recruit many supporters and mentors that will...skills too. Teach classes Go to workshops and conferences on interviewing, resume writing, and networking...

...the researchers applied the LITE system to 28 different genes to establish it as “a novel mode of optogenetic...activators are easy to expand or combine with different effectors. I’d encourage people to get creative...readers, how will you get creative with LITE? Reference: Silvana Konermann et al. "Optical control of ...

...internship would be a perfect way of exploring my different interests but also a way of challenging what I...would look like. While working remotely was a different experience, It was helpful to have Addgenies reassure...spoke with amazing people that were a part of the different departments of the company. I asked questions ...

... a given mutation will have a different phenotypic effect in a different system. These plasmids can also...well-studied candidates. The possibilities are vast. References Ng, Patrick Kwok-Shing, et al. "Systematic functional...

...nervous system: infected grasshoppers expressed different proteins in their brains compared to uninfected...and geotactic behavior (Biron et al., 2005).   References Biron, D. G., et al. "Behavioural manipulation...Isopoda)." Copeia 1983.3 (1983): 813-816. Gilbert, Lawrence I. "Halloween genes encode P450 enzymes that mediate...

... gRNA table You can access the datatable here. Current table columns are target gene, species, sequence...ID, and depositor. The CRISPR applications we currently have data for are: cut, activate, interfere, visualize...jointly by Brook Pyhtila and Nicole Waxmonsky. References McDade J, Waxmonsky NC. Emerging CRISPR Technologies...

...section!   References 1. Yearly Planning Meetings: Individualized Development Plans Aren't Just More Paperwork...you can identify suitable careers based on your current interests and skillset. With this information in...

... might not fold well, you should screen a few different vectors to see which gives you the most functional...two sets, each of which includes plasmids with different varieties of tags, both N- and C-terminal. Each...individually or as a complete kit from Addgene. References 1. Goulas, Theodoros, et al. "The pCri system...

...LA, where it is currently on display.   Many universities are now following the trend and encouraging... biology and I believe it could have made the difference in some areas of conservation.” Appreciating ...

... by Daniel Bauer and Matthew Canver of Boston Children’s Hospital and Harvard Medical School. Addgene ...hematologist and principal investigator at Boston Children’s Hospital and Harvard Medical School. His lab ...Bioengineering from the University of Pennsylvania.     References: 1. Lee HJ, Kim E, Kim JS. Targeted chromosomal...

...Addgene grow into the successful, internationally renowned plasmid repository that it is today. Although ...faces of some of our depositing scientists who weren’t able to join us in person. Joanne Kamens, Melina...entire night was when Melina and Ken called their parents up to the podium to be recognized. John and Stephanie...

...lots of interesting things from children, their classmates, and parents”. He also cooks and cleans with...academics because it offers more flexibility and you aren’t locked into a schedule.” George Church comments...

...post-its to make finding your most used protocols & references easy to find It’s happened to all of us. It’s...consider using color coded binders and tabs (for different experiments or projects) to make it easier to ...small by identifying the protocols, guides, or references that your labmates ask to borrow most frequently...

...in diabetes and lymphoma research or the many different ways you can improve your heart health. These .... Like other forms of mesothelioma, there are currently no targeted treatments for peritoneal mesothelioma...rare disease research funding opportunities. References 1. Jones, C., et al. "PP09. 1–2352: Systematic...

...With renewed energy and optimism that comes at the start of every new year, it is a wonderful opportunity... from 1,106 labs in 504 organizations from 37 different countries, into our repository, while expanding... We will pursue opportunities to build on our strengths that have successfully served the scientific community...

...assistance with this blog post. References and resources References Feng, J., Zhang, C., Lischinsky, ... nLight nLight sensors are based on alpha-1a adrenergic receptor; nLightG contains circularly permuted...

... to use the same peptide tag for all sorts of different protein systems and they shouldn’t get in the ...marker expression level. Labs have been trying different approaches to improve CTC capture, but I hope ...Addgene. I am a big fan; thanks for the T-shirt! References: SpyLigase peptide-peptide ligation polymerizes...

... more than 75,000 plasmids to more than 1,500 different institutions in Europe. In the last four years...over the first five months of 2014 reaffirms this trend. In addition to this, more and more European scientists...focused on supporting European scientists. Born a french frog, I moved to Albion six years ago to work as...

...guest blogger, Meghan Monroy. Meghan Monroy is currently an Outreach Data Curation Intern at Addgene. She...Connecticut interested in protein science.     References: 1. Celie, Patrick HN, Annabel HA Parret, and...Recombinant cloning strategies for protein expression." Current Opinion in Structural Biology 38 (2016): 145-154...

...fundamentals. Our mission is to curate a one-stop reference guide for plasmids, so that you can spend less...plasmid use in the lab. Addgene’s Molecular Biology Reference includes information about molecular cloning, ...a plasmid vector, molecular biology tools and references, and how to maintain your plasmid stocks. The...

... the biomedical research field! References and Resources References Fellmann, C., et al., Cornerstones...knockout in a cancerous background and treat the parental cell line and the X knockout with your drug(s)...

...science writing is clear, Caitlin’s career plans weren’t concrete when she finished grad school at the University...enjoyed the writing and editing sphere.” But things aren’t always that simple, especially when your spouse...growing in the science sphere, but the journals aren’t out here. They’re really East Coast. When we first...

...feedback (may be the same as self-assessment or different): Here are the 3 things I would say worked out...you needed? What could I (manager) have done differently? What Can You Learn Going Forward: If you could...could do the thing all over, what would you do differently? Beware of indirect language You must be honest...

...in this series. Check out Joanne's Reddit AMA Different types of mentors for grad school vs. postdoc Choosing...Choosing an advisor to complete a successful PhD is different from choosing a lab for postdoctoral work. However...think, “Oh, but that won’t happen to me – I am different.”  Unfortunately, a bad mentor can be bad news...

... if I would have formulated specific comment differently if I would be speaking to a colleague in the ...of Arabidopsis HapMap population in Amsterdam. Currently, she is a postdoc at KAUST, Saudi Arabia, in the...own research group at Boyce Tompson Institute.  References Baldwin, Melinda. "Credibility, peer review, ...

...why this protein was blue-shifted compared to its parent protein CFP484. Collaborating with scientists from... are fused to. The researchers constructed 22 different mTFP1 fusions to both the C- and N- termini of...community, please deposit the plasmids at Addgene! References Ai, Hui-wang, et al. "Directed evolution of a...

...combinatorial expression of these three genes with different copy numbers, producing dozens of color hues (...2013). However, the brightness of Brainbow was inherently low. This is because the stochastic and combinatorial...website: https://dn-en.med.kyushu-u.ac.jp/     References Cai, Dawen, et al. "Improved tools for the Brainbow...

...each of the gRNAs. The idea was to assign four different barcodes to each of the gRNAs and to embed these...of the recombination sites in the construct to different lengths, the density of cells that are labeled...identify new antifungals that target calcineurin. Current calcineurin gene reporters are based on the bacterial...