Skip to main content

We narrowed to 868 results for: REN

Showing: 541 - 570 of 868 results
  1. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...of conversations with people who work in many different scientific fields. They also host AMA forums where...first makes Reddit a great platform for those who aren’t so great at coming up with answers off the cuff...future. The AMA was also cataloged for permanent reference by the Winnower. The only small downside to the...leaving out the term “implicit bias” which is apparently a troll trigger phrase these days). Redditors...
  2. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...testing is crucial in controlling this pandemic. Currently, the CDC uses RT-qPCR tests to diagnose COVID-...Cas12a, Cas13a does not require strict sequence preferences at the target site, while Cas12a requires a PAM...femto-aptomolar range. For more details on the differences between these methods, check out this table.  ...can follow her on Twitter @shra_krishna.       References Azhar, M., Phutela, R., Ansari, A. H., Sinha,...
  3. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ... involved multiple people in tight locations. Conference rooms for on-screen presenters! Labs and benches...Zoom, everyone’s new favorite third-party video conferencing software. Interviewees could simply boot up ...while the camera is here, the operator is in a different room.   Design: The previous cycle made me...monitor and teleprompter being repurposed for a different shoot to create the How to Deposit Plasmids video...
  4. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...with transcriptional activation. Created with BioRender.com. Tet1 demethylase Ronggui Hu’s lab has created... with increased transcription. Created with BioRender.com. Tet1 initiates cytosine demethylation of...with transcriptional repression. Created with BioRender.com. DNA Methyltransferase MQ1 Margaret Goodell's...Schwiesow contributed to updating this article. References Haldeman JM, Conway AE, Arlotto ME, et al (2018...
  5. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...influence the evolution of their bacterial prey. Currently, receptor binding proteins (RBPs, the host recognition...thinking. Find her on Twitter @JessicaSacher.   References 1. Nobrega, Franklin L., et al. "Revisiting phage...phage therapy: new applications for old resources." Trends in microbiology 23.4 (2015): 185-191. PubMed PMID...bacteriophage-encoded glycan binding proteins and carbohydrates." Current opinion in structural biology 34 (2015): 69-77....
  6. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ... strategies in C. elegans As brilliantly and irreverently illustrated in the parable of the Geneticist...the sequence added, leaving a 34 bp “scar”, but currently requires additional experimental manipulation....days. The only animals on a plate, other than the parental animal, are rescued progeny, which makes screening...more at his webpage or follow him on Twitter.   References Arribere JA, Bell RT, Fu BXH, Artiles KL, Hartman...
  7. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...more common luciferases like firefly (FLuc) and Renilla (RLuc) luciferase. This is important not so much...strategy was first applied using RLuc8, an engineered Renilla luciferase, to produce a series of FP-RLuc8 fusions... #85201), Orange (OeNL; plasmid #85202) and Red (ReNL; plasmid #85203) NLuc fusions. The Nagai lab has...Promega cellular analysis products at Promega.     References 1. Hall, M.P., et al. (2012) Engineered luciferase...
  8. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...excitation light path. Each hole corresponds to a different location in the sample. As more than one hole ...one of four categories that are each suited to different types of modern day fluorescence microscopes (...means that the images will appear sharp without interference from blurry out-of-focus light. The most difficult...microscopy manufacturers have similar information References 1. Lichtman JW, Conchello JA. Fluorescence microscopy...
  9. Find and Share AAV Data with Addgene's New AAV Data Hub

    Type
    Blog Post
    Published
    Oct. 8, 2019, 12:33 p.m.
    ...over 100 entries spanning six different species and dozens of different expression sites. The Data...the AAV Data Hub is a beta project, and we are currently only accepting data for materials distributed ...
  10. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    Published
    Oct. 31, 2024, 1:15 p.m.
    ...search engines, which allow you to search many different antibody catalogs at the same time.  If you're...Library for Cancer Research Human Luekocyte Differentiation Antigen Workshops Immune cells Flow cytometry...others) Data repository From the Human Cell Differentiation Molecules group Addgene Data Hub Any ...
  11. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ...has deposited If you’d like to write about a different topic, feel free let us know. What do you get ...audience of biological scientists from a variety of different fields. Exposure - With over 100,000 views each...interact with and discuss your work with many different people. Recent Guest Writers Have Included: Wenning...
  12. Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service

    Type
    Blog Post
    Published
    July 28, 2020, 1:15 p.m.
    ...that this service will be helpful to the many different scientific communities using plasmid tools. Details... our Plasmids 101 blog series Learn about the different grades of DNA  Read about the various ways to ...other beta projects Read our Molecular Biology Reference ...
  13. Interview with Bart Newland, Addgene’s Newest Board Member

    Type
    Blog Post
    Published
    Aug. 3, 2021, 1:15 p.m.
    ...demand. Q: Can you tell me a bit about your job currently? What is your day-to-day like? BN: I've made the...large companies, Genzyme and Biogen. They’re very different jobs. Being general counsel at a small start-up...companies. It's one of those "every day is something different" type of jobs, which really suits my personality...
  14. Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana

    Type
    Blog Post
    Published
    Dec. 20, 2016, 3:30 p.m.
    ... useful products under Professor Christopher E. French. Dr. Duedu was recently awarded plasmids from Addgene...7:12 - 7:11 : Kwabena's work in Christopher E. French's Lab at the University of Edinburgh - synthetic...bioresource to attract research funding. Using current resources to develop microbiology and molecular...
  15. Fueled by Coffee at #SfN14

    Type
    Blog Post
    Published
    Nov. 18, 2014, 7:15 p.m.
    ...more than 30,000 neuroscientists over a 5 day conference? I feel like here at the annual Society for Neuroscience...late, late night socials. As a newbie to the SfN conference, I have been impressed by how the neuroscience...reports from bloggers at the meeting – my report is currently in the works. Or follow #SfN14 on Twitter for ...
  16. What's New in CRISPR - May 2019

    Type
    Blog Post
    Published
    May 28, 2019, 12:49 p.m.
    ...AAV constructs CRISPR interference in Candida albicans A new CRISPR interference (CRIPSRi) platform for... to Cas9 SECURE base editors Nme2Cas9 CRISPR interference in Candida albicans E. coli genome-wide inhibition...
  17. More Data for You: Find Articles Citing Addgene Plasmids

    Type
    Blog Post
    Published
    June 16, 2015, 2:15 p.m.
    ...validated in.  If a plasmid's Addgene ID # has been referenced in other publications, you'll find a link to ...Additional features Once you've clicked on the "# References" link under the "Resource Information" heading...Addgene’s Plasmids 101 eBook Protocols and Plasmid References @Addgene  ...
  18. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ...splicing, imaging, and more. Abil says she is currently working on a version that she hopes will change...PUF proteins lose their solubility. Her lab is currently investigating the issue and hopes to have a better...happy to share updated versions of the kit.” Reference Abil Z, Denard CA, Zhao H.  Modular assembly of...
  19. Developing Transferable Skills During Science Training

    Type
    Blog Post
    Published
    June 3, 2014, 2:14 p.m.
    ...colleagues you help will be future advocates and references. Collaborate with another lab, in another field... a committee and do something big like plan a conference Recruit many supporters and mentors that will...skills too. Teach classes Go to workshops and conferences on interviewing, resume writing, and networking...
  20. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...the researchers applied the LITE system to 28 different genes to establish it as “a novel mode of optogenetic...activators are easy to expand or combine with different effectors. I’d encourage people to get creative...readers, how will you get creative with LITE? Reference: Silvana Konermann et al. "Optical control of ...
  21. Stepping into a Virtual Internship: My Experience at Addgene

    Type
    Blog Post
    Published
    Aug. 13, 2020, 1:21 p.m.
    ...internship would be a perfect way of exploring my different interests but also a way of challenging what I...would look like. While working remotely was a different experience, It was helpful to have Addgenies reassure...spoke with amazing people that were a part of the different departments of the company. I asked questions ...
  22. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    Published
    Oct. 31, 2019, 1:06 p.m.
    ...nervous system: infected grasshoppers expressed different proteins in their brains compared to uninfected...and geotactic behavior (Biron et al., 2005).   References Biron, D. G., et al. "Behavioural manipulation...Isopoda)." Copeia 1983.3 (1983): 813-816. Gilbert, Lawrence I. "Halloween genes encode P450 enzymes that mediate...
  23. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ... might not fold well, you should screen a few different vectors to see which gives you the most functional...two sets, each of which includes plasmids with different varieties of tags, both N- and C-terminal. Each...individually or as a complete kit from Addgene. References 1. Goulas, Theodoros, et al. "The pCri system...
  24. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ...LA, where it is currently on display.   Many universities are now following the trend and encouraging... biology and I believe it could have made the difference in some areas of conservation.” Appreciating ...
  25. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    Published
    Feb. 18, 2015, 3:09 p.m.
    ... by Daniel Bauer and Matthew Canver of Boston Children’s Hospital and Harvard Medical School. Addgene ...hematologist and principal investigator at Boston Children’s Hospital and Harvard Medical School. His lab ...Bioengineering from the University of Pennsylvania.     References: 1. Lee HJ, Kim E, Kim JS. Targeted chromosomal...
  26. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Published
    July 3, 2014, 3:48 p.m.
    ...Addgene grow into the successful, internationally renowned plasmid repository that it is today. Although ...faces of some of our depositing scientists who weren’t able to join us in person. Joanne Kamens, Melina...entire night was when Melina and Ken called their parents up to the podium to be recognized. John and Stephanie...
  27. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    Published
    May 8, 2014, 3:07 p.m.
    ...lots of interesting things from children, their classmates, and parents”. He also cooks and cleans with...academics because it offers more flexibility and you aren’t locked into a schedule.” George Church comments...
Showing: 541 - 570 of 868 results