We narrowed to 833 results for: Cat
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TypeBlog PostPublishedFeb. 5, 2019, 1:04 p.m....involves a few key features that can be difficult to replicate in other work settings: The sense of being the... might include collaborating, managing, and communicating. After years of focusing on my technical skills...
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Plasmids 101: Codon usage bias
TypeBlog PostPublishedSept. 27, 2018, 1:09 p.m....ribosome may therefore stall at inappropriate locations or fail to make it through the entire transcript...generally segregate with the cell pellet during purification procedures. Even if you produce a large amount... -
CRISPR Activation: A Practical Guide
TypeBlog PostPublishedAug. 18, 2016, 2:30 p.m.... (2014) A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging.....org Find Cas9 Activators Find Cas9 Repressors Catch Up on Your CRISPR Background with Our CRISPR Guide... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog PostPublishedSept. 17, 2019, 12:40 p.m....have made great strides to map brain regions and allocate specific functions to them. Electrical recordings...long-lasting antidepressant effects." Nature communications 10.1 (2019): 223. PubMed PMID: 30644390. PubMed... -
Tips for Using FRET in Your Experiments
TypeBlog PostPublishedNov. 5, 2014, 3:41 p.m....allows the detection of molecules' interactions, modifications or dissociations in situ. Used since the mid...14. Zimmermann T, Terjung S. FRET Basics and Applications: An EAMNET Teaching Module http://www.embl.de... -
Five Popular Model Organisms
TypeBlog PostPublishedApril 11, 2019, 12:39 p.m.... throughout their lifetime. Thus any genetic modification, such as expression of fluorescent protein or...studying somatic mutations and cancer." Nature Education 1.1 (2008): 183. Zuber, Johannes, et al. "Mouse... -
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models
TypeBlog PostPublishedApril 5, 2018, 12:42 p.m.... allele To design a knock-in allele at a given location, you’ll need to design only a single guide and... out our CRISPR Guide Find Plasmids for Cas9 Purification Find CRISPR Plasmids for Mammalian Expression... -
Antibodies 101: Designing Your First Flow Panel
TypeBlog PostPublishedMay 28, 2024, 1:45 p.m....reporter, such as GFP or mCherry, through genetic modification. As your protein of interest will only occur...excitation and emission. The visible color spectrum is located in a range of wavelengths of about 380–700 nm. ... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog PostPublishedAug. 15, 2017, 1:24 p.m.... required for a growing organism to make the complicated transition from a clump of cells to a fully differentiated...relatively little tissue absorption, weak tissue scattering and small amounts of tissue auto-fluorescence... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog PostPublishedJuly 11, 2017, 2:30 p.m....the user. This approach is useful for several applications as will be discussed below. The software could...contain multiple gRNAs targeting all genes in the indicated species and were suggested to be the best gRNAs... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog PostPublishedNov. 6, 2018, 1:12 p.m....joined Cell Press to pursue a career in science communication and publishing after completing her postdoctoral...PMC4096605. 30. Lamba, D.A., et al., Generation, purification and transplantation of photoreceptors derived... -
New Western Blot Protocol Video!
TypeBlog PostPublishedMarch 28, 2023, 1:15 p.m.... Western blot and share tips for this tricky application. You can check out all of our protocols ... -
Meet Aavery, Blugene's New Virus Friend!
TypeBlog PostPublishedFeb. 13, 2020, 2:34 p.m....place in our hearts: our first two offices were located in Kendall Square in Cambridge, MA. We hope you... -
Suggest A Plasmid You'd Like to Find at Addgene
TypeBlog PostPublishedMarch 16, 2016, 5:30 p.m....suggestaplasmid, the name of the plasmid and a publication where we can find the plasmid. While we cannot... -
The 12 Days of CRISPR: 2021
TypeBlog PostPublishedDec. 28, 2021, 2:15 p.m....On the ninth day of CRISPR, Addgene gave to me: MUCICAT to perform multicopy chromosomal integration. ... -
We’re Going Viral This Week!
TypeBlog PostPublishedOct. 14, 2019, 1 p.m.... information on the basics of viral vectors, applications, and some new resources available from Addgene... -
Enabling high school research at the Journal of Emerging Investigators
TypeBlog PostPublishedSept. 5, 2018, 12:01 p.m....Additional Resources from the Addgene Blog Find education resources Use our Plasmids 101 eBook to teach ... -
Addgene at SfN23!
TypeBlog PostPublishedNov. 9, 2023, 2:15 p.m....Finally, our team is rounded out with our science communication specialist (you'll know me by my Tweeting — ... -
Find and Share AAV Data with Addgene's New AAV Data Hub
TypeBlog PostPublishedOct. 8, 2019, 12:33 p.m....important details that you won’t find in the final publication and we have already collected over 100 entries... -
8 Great Depositor Research Stories
TypeBlog PostPublishedDec. 15, 2022, 2:15 p.m....Sapozhnikov tells us how to use dCas9 (with no modifications!) as a CRISPR/dCas9-based tool for DNA demethylation... -
Twelve Amazing Guest Blog Contributors!
TypeBlog PostPublishedNov. 26, 2024, 2:15 p.m....Modular Cloning and Plasmids 101: Modular Cloning Applications and Kits We are very grateful for each and... -
Call for Guest Bloggers
TypeBlog PostPublishedMarch 2, 2016, 3 p.m.... to any molecular biologist Tips for protein purification Replica plating Anti-CRISPR Retroviral vectors... -
#DeckTheLab with Tinsel and Test Tubes, Fa La La La La La La La La
TypeBlog PostPublishedDec. 2, 2021, 2:15 p.m....create a stack of wrapped gifts using life science catalog pages. Make your WFH space feel a little more festive... -
Streaking for Single Colonies: The Streak Plate Challenge
TypeBlog PostPublishedMarch 2, 2021, 2:15 p.m....blog posts on fluorescent proteins Check out educational resources on the blog Resources on Addgene.org... -
Activate Addgene's "Find Plasmids" Badge on Pubmed Abstracts
TypeBlog PostPublishedMay 12, 2017, 12:58 p.m....right). Once on the “Filters” page, select the category “LinkOut” and search for “Addgene”. Then check... -
Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More
TypeBlog PostPublishedNov. 15, 2016, 3:30 p.m....materials (check out our new Viral Service), and educational outreach. Many thanks to Alex Chavez for taking... -
New Viral Vectors - March 2024
TypeBlog PostPublishedMarch 5, 2024, 2:15 p.m....vectors in the Addgene repository Name Serotype Category Depositor Notes AAV-CAG-jGCaMP8s-WPRE AAV1 ... -
Newly Updated AAV Data Hub!
TypeBlog PostPublishedFeb. 7, 2023, 2:15 p.m....tropism* (min. 300 dpi, PNG format) At least two replicates of your experiment* Functional data, if the report... -
New Viral Vectors - Summer 2024
TypeBlog PostPublishedJuly 9, 2024, 1:15 p.m....vectors in the Addgene repository Name Serotype Category Depositor Notes pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE... -
Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service
TypeBlog PostPublishedJuly 28, 2020, 1:15 p.m....streaking to a single colony to DNA extraction and quantification so that you can focus on what you want to do... -
2018 #DeckTheLab Contest Winners
TypeBlog PostPublishedJan. 15, 2019, 2:19 p.m....wearestemsquad #festivefun #phdlife #phdtips #sciencecommunication #sciencedaily #instascience #ladies #research... -
Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana
TypeBlog PostPublishedDec. 20, 2016, 3:30 p.m....health research, the HEAD outreach program, and educating the public about dietary health. 40:17 - 44:14... -
Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs
TypeBlog PostPublishedJune 15, 2016, 1:30 p.m....concluded, Addgene and Seeding Labs will be opening applications for a second round of plasmid awards on June... -
Addgene’s Top 10 Blog Posts from 2014
TypeBlog PostPublishedDec. 16, 2014, 8:01 p.m....Doench (Guest Blogger) Plasmids 101: Origin of Replication By Kendall Morgan Save Time and Money by Making... -
What's New in CRISPR - Winter 2018
TypeBlog PostPublishedDec. 18, 2018, 1:17 p.m....monomeric Cas9’s. Read the paper in Nature Communications Find the Cas9-Cas9 chimera plasmids A modular... -
CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping
TypeBlog PostPublishedNov. 29, 2018, 1:53 p.m....of dsDNA and RNA in trans. Fluorescent signal indicates the presence of this viral sequence in the assay... -
Your Top Requested Plasmid in 2016!
TypeBlog PostPublishedJan. 11, 2017, 8:47 p.m....as links to the Zhang Lab's Nature Protocols Publication describing them here. References 1.Ran, F. Ann... -
Designer PUF Proteins for Any RNA Target
TypeBlog PostPublishedJune 17, 2014, 7:57 p.m....says the new tool might even find therapeutic application in addition to their use in basic biology, for... -
Stepping into a Virtual Internship: My Experience at Addgene
TypeBlog PostPublishedAug. 13, 2020, 1:21 p.m....right path. The frequent Zoom meetings helped me communicate my ideas. I was able to express my visions for... -
Top Requested AAV of 2017: pmSyn1-EBFP-CRE
TypeBlog PostPublishedJan. 17, 2018, 2:57 p.m....pmSyn1-EBFP-Cre Of course there are also more complicated ways to use this particular AAV. For instance...