We narrowed to 801 results for: DES

...retroviral CRISPR knockout library using gRNAs designed by the Kosuke Yusa Lab. This retroviral library...

...bacterial proteins that bind serotonin. Unger redesigned the binding pocket of the periplasmic binding...

...producer cells and purification protocols often describe a process that begins with cell lysis to release...

...next-generation sequencing (NGS). For gRNA libraries, you’ll design primers to create 200-300 bp sequencing products...

...shipment processes. After reading a publication describing how short term storage on dry ice can shift sample...

...on social media or the phones hiding under their desks than listening to me. When I got home, I saw my ...

...store your plasmid DNA Learn about the different grades of plasmid DNA preps Resources on Addgene.org ...

...on Twitter where each poster presenter had four “slides” to use for their poster, eliminating the need ...

...with NEB enzymes, the app is quite helpful and provides a list of directions including which buffers to...

...of R bodies R bodies have been studied for many decades, and they were known to be resistant to detergents...

...Define the scope of the meeting: Are you meeting to design an entire experiment and delegate tasks to lab ...

...ability to influence editing efficiency. In 2022, despite the increasing effectiveness of CRISPR/Cas9, scientists...

...characterization of the new variants is available here. Upgrades to iGluSnFR3 To improve upon the SF-iGluSnFR, ...

...to note that it’s the depositing institute that decides the terms of distribution for their plasmids. ...

...University of British Columbia, Student Services - includes videos on general resume writing, as well as additional...

...run on an agarose gel. The resulting gel image includes a 1kb ladder (lane 1) that has bands ranging from...

... a Good Mentor for Scientists". This post also includes a useful list of questions for a candidate to ...

...different phenotypes. For example, this plasmid encodes a green fluorescent protein gene which makes the...

...2A-Puro (PX459) - This mammalian expression vector encodes both SpCas9 and your gRNA. It’s the former version...

... another way, only cells or organisms with the desired phenotype will survive and be assayed at the end...

... that you know how to read flow plots and have designed your first flow panel, you’ll load your samples...

... from University of Calgary. Over the past few decades, genetically encoded calcium indicators (GECIs)...

...focusing on molecular biology and metabolism. He now guides fellow PhDs in their careers with actionable steps...

...about a specific virus? We have you covered with guides on lentivirus, retrovirus, adenovirus, and AAV....

...Bhopal. All illustrations / figures featured were designed by our team member Kallol Mozumdar. Sreepadmanabh...

...even if science writer Ed Yong’s book “I Contain Multitudes” has me considering the microbiome more often...

... don’t overdo it (some of my best studies were designed over late night poker games at Gordon Conferences...

...have been a model organism for plant research for decades, and the biochemical pathways involved in central...

...to the development and spread of cancer cells. Besides these stromal cell modifications, stromal cells...

... phosphatase activity: In yeast, the PHO8 gene encodes a vacuolar alkaline phosphatase. Normally PHO8 ...

...the postdoctoral population which keeps growing despite a lack of available academic positions.   Many...

...novel RNA-protein interactions, Ramanathan et al. designed an RNA bait containing three well conserved but...

...these difficult pieces: “It’s probably the best.” Besides working through the tough assignments, the skills...

...Allewell and Sarma, 1974). Additionally, ThermoFisher provides a protocol on how to integrate RNAlater® with ...

...affinity, many indirect antibody protocols are designed to take place over multiple days. Increased run...

...talking about here? Many of the Fab fragments described above can be used for similar applications and...

...down your own results; are they the same as the description the authors give? Ask yourself if the results...

...Activation of membrane receptors and signaling cascades conceivably allows the genome to sense the impact...

...uncomfortable places, from sticky ethical dilemmas to dark episodes in the history of eugenics and research misconduct...

...guanidine moiety at the end of its side chain. Decades ago, other labs discovered that the in vitro functions...