Skip to main content
Addgene
Showing: 721 - 760 of 780 results

  1. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...making it well suited for AAV packaging. Most importantly, this CasMINI system was shown to be effective...

  3. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...similar to that of a comparable FISH method. Importantly, they did not observe labeling when a. no gRNA...

  8. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...like Gateway cloning or Gibson assembly. Another important consideration is the design of flanking overhangs...

  9. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ... Plasmids 101 series, we examined a number of important plasmid elements – promoters, origins of replication...

  11. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...phenotype, translational output, etc., which can support a larger cellular engineering goal, several of ...

  17. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...the sample regardless of whether or not it is incorporated into a viral particle. Consequently, titers ...

  19. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...muscle groups (usually the thigh), though it is important to avoid injection into the sciatic nerve. Mice...

  20. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...different titers are measured. A TCID50 measurement reports an infectious titer, meaning the concentration ...

  21. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ...change your compensation values during the analysis portion of your experiment. So, as long as you are able...

  22. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...passage are too young and should not be used for important transfection experiments right after they’ve been...

  23. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...expression. Despite their ease of use, it is important to note that bacteria usually cannot produce functional...

  25. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ... can find it in likely mean it will remain an important tool for the foreseeable future. These newer findings...

  26. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ... lab has created a novel nanocompartment for transporting proteins in yeast by using murine polyomavirus...

  33. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ... in copy number, the number of plasmid copies supported within each bacterial cell in your culture. Copy...

  34. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...addition, the use of viruses allows much better spatiotemporal control over labeling methods as compared to...

  35. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...you have used blastn to determine the reliable portion of a Sanger sequencing result and noted any potential...

  36. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...temperature). pH Stability: This parameter is important if you are planning to express the FP in acidic...

  37. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...26 of a 30 nt target sequence. For the editing portion of the protein, they examined ADAR1 and ADAR2, ...

  39. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...Purification and Gel Extraction Kits. Scientific Reports. https://doi.org/10.1038/s41598-018-30316-w  Zou...
Showing: 721 - 760 of 780 results