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We narrowed to 860 results for: fer

Showing: 721 - 760 of 860 results

  1. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...have made it easier to track translation in two different ways: by monitoring the first round of translation...various cell types and physiological states. References 1. Ingolia, Nicholas T., et al. “Genome-wide ...

  2. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...restriction enzymes since the instructions use NEB buffers. Nonetheless, if you are setting up a double digest... provides a list of directions including which buffers to use and that indicates if a sequential digest...

  3. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...inactive state. The dynamic range, defined as the difference between the lowest and highest level of “output...various scientific or engineering applications.   References Refactoring and optimization of light-switchable...

  4. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ...of prominent members of the bioscience and tech transfer communities who help guide and promote Addgene... of how miRNAs regulate their targets and what confers specificity in the miRNA regulatory process. This...

  5. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ... proteins whose excitation and emission spectra differ sufficiently for them to be imaged in distinct ...fluorescent channels on the microscope. With the proliferation of fluorescent proteins in recent years, there...

  6. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...method’s flexibility allows it to be used with different types of PCR-generated inserts. By harnessing ...updated by Emily P. Bentley in September 2024.  References and Resources Further reading Harnessing homologous...

  7. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...high and your immunogenicity low! References and Resources References Robinson HL. Retroviruses and cancer...

  8. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ... the cutting. While sonication is usually the preferred method, enzymatic digestion can be appropriate...protocols when embarking on your ChIP journey!  References and Resources More resources on Addgene’s blog...

  9. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ... web of opportunities that the classroom cannot offer. Furthermore, companies across most industries tend...helps you find internships and scholarships in different subject areas!    2. Resume and cover letter...

  12. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...and SV40 promoters, are always active and thus referred to as constitutive promoters. Others are only ...Experimental System Because transcription machinery differs between cell types or organisms, promoters are ...

  13. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...the system and the flexibility to address many different types of research questions. Are you ready to ...experiments? Find EvolvR plasmids at Addgene! References Gaj, Thomas, and Pablo Perez-Pinera. "The continuously...

  15. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ... and precisely identifies plasmid features that differ by only a few nucleotides, such as the fluorescent...static image file (PNG, see above) for your own reference or to paste directly into your notebook. This ...

  16. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ...peptide. By combining three different localization peptides with four different fluorescent proteins, and...

  17. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...This post was originally written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered...period. For ease of calculations*, let’s split the difference and put scientific inflation at… roughly 34.9%...

  19. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...us develop these resources further. Feel free to offer your comments and suggestions, and even to submit...submit a guest blog entry of your own! References 1. Bernard, P. “Positive Selection of Recombinant DNA ...

  20. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ...function of the protein and pdDronpa are preserved. References 1. Zhou, Xin X., Linlin Z. Fan, Pengpeng Li, ...28232577. Figures 2-4 were modified from this reference. 2. Regot, Sergi, Jacob J. Hughey, Bryce T. Bajar...

  23. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ...and industry jobs. Since there are a variety of different career paths that don’t all need another degree...-op. It has been so insightful to learn about different career paths, successes and failures, values, ...

  24. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...virtual bookshelf. Pro tip The “cited by” and references sections of a PubMed entry are great places to...This library can also be synced with a Mendeley reference manager account. Pro tip Sign up for weekly email...

  25. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ... to address the unique needs of students from different departments across the university and alterations...public policy. Follow David on Twitter @driglar.   References: 1. Alberts, Bruce, et al. "Rescuing US biomedical...

  26. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...academic labs. Being unable to publish or attend conferences makes it very challenging to compete in today...pressure to publish and more freedom to attend conferences, but after receiving her degree she was faced...

  28. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...species, like S. aureus and N. meningitidis require different PAM sites than SpCas9, in this case, NNGRRT and...is working for you in the comments section! References Hu, Johnny H., et al. "Evolved Cas9 variants ...

  29. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...explaining CRISPR technology to 4th graders, check out Jennifer Doudna’s new book A Crack in Creation. Making... the target DNA strand, and then activate two different endonuclease active sites. Fortunately, there ...

  30. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...against improper, random recombination into a different locus. Correctly recombined cells will not contain...should increase. New genome engineering tools also offer hope for the creation of new knockout animal models...

  31. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ... the process is similar to academia. The only difference is that your paper will be reviewed by an internal...LinkedIn connections and other contacts to get that referral and find out more about the company’s program....

  32. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...human genome-wide CRISPRko (knock out), CRISPRi (interference), and CRISRPa (activation) libraries that do...conditions such as model systems and primary cells. References 1. Doench, John G., et al. "Rational design of...

  33. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...expertise with our district middle school was a very different experience since, unlike elementary schools, middle...such a valuable extension to our curriculum and offered engaging examples of how understanding genetics...

  34. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...comes down to the researcher’s familiarity with different systems.  Using Cas9-gRNA RNPs in your experiments...include genome engineering and microbiology.     References 1. Chen, Sean, et al. "Highly efficient mouse...

  35. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...truth, people are incredibly varied in how they prefer to consume information. Personally, I would much...outcome to the limitations and potential of your preferred platform before you start can save a lot of trouble...

  36. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...mixed population, represented here by cells of different colors, specifically targets bacteria with the...ground in the fight against bacterial superbugs. References 1. Citorik, Robert J., Mimee, Mark, and Timothy...

  37. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...Joanne’s back yard this year, though she generously offered. Instead we picniced outside of our new office ...Netflix series. The thing that has made all the difference here at Addgene is the emphasis placed on team...

  38. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...Red-shifts the wavelength (yellow derivatives) A206K Interferes with dimer interface (monomeric derivatives) ...earliest uses of GFP. Fluorescence resonance energy transfer (FRET): This is used to study the interactions...

  40. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...existing comprehensive mutagenesis methods, PFunkel offers the best combination of library coverage, mutational...mutagenesis? How would you use it in your research? References 1. Natarajan, Chandrasekhar, et al. "Epistasis...
Showing: 721 - 760 of 860 results