960 results

Learn about using SpyLigase for irreversible peptide-peptide ligation and magnetic cancer cell capture.

Aug. 13, 2014, 3:49 p.m.

How to make the most of your time at a scientific conference. Tips for making friends and networking before, during, and after the conference.

Aug. 7, 2014, 1:58 p.m.

Jim Woodgett discusses the data sharing policies of journals, such as PLOS, and how access to data affects scientific research.

Aug. 5, 2014, 6:51 p.m.

The Michael Davidson lab deposited their collection of 3,300 fluorescent protein plasmids including empty backbones to Addgene.

July 29, 2014, 6:16 p.m.

A look at the challenges facing the field of plant synthetic biology, including a discussion of changing public perception, IP concerns, and more.

July 22, 2014, 5:46 p.m.

Neil McKenna and Bert O’Malley of the Nuclear Receptor Signaling Atlas (NURSA) provide an introduction to nuclear receptors and more.

July 15, 2014, 4:03 p.m.

Advice on starting a biotech company from how to take an idea developed during graduate school and turn it into a startup.

July 10, 2014, 7:17 p.m.

Addgene's mascot, Blugene, gives you an inside peek at Addgene's 10 Year Anniversary Gala.

July 3, 2014, 3:48 p.m.

Learn what an informational interview is, what it is not, and how scientists can use it to their advantage during their career.

July 1, 2014, 3:42 p.m.

Find tips for using the chromatogram to analyze your Sanger DNA sequencing results.

June 26, 2014, 2:23 p.m.

The PUF Assembly Kit from Huimin Zhao's lab makes it possible to devise designer PUF proteins to hit any RNA target of interest.

June 17, 2014, 7:57 p.m.

Interview with Nicola Patron about her work in plant synthetic biology and her Golden Gate MoClo plasmid kit.

June 12, 2014, 3:30 p.m.

Information about Addgene's new Europe office located in London and operated in collaboration with LGC.

June 10, 2014, 3:20 p.m.

The co-founders of Revolution Bioengineering, aka RevBio, share the story of how they chose to leave academia and start a synthetic biology company.

June 5, 2014, 3:32 p.m.

Advice for things you can do to develop transferable skills while you are also learning to be an excellent scientist.

June 3, 2014, 2:14 p.m.

Tips to help you save time when using the the Basic Local Alignment Search Tool (BLAST) provided by NCBI to compare sequence results.

May 29, 2014, 1:29 p.m.

Tips to consider when publishing a scientific manuscript, including what type of journal to publish in, peer review, and helpful dos and don'ts.

May 22, 2014, 4:02 p.m.

Tips to help you choose which fluorescent protein to use in your experiments.

May 20, 2014, 2:06 p.m.

Green fluorescent protein (GFP) and its derivatives can be used experimentally as fusion tags, biosensors, reporters, and more.

May 15, 2014, 3:33 p.m.

Ten ways to begin sharing your science with your community, from talking to writing to tweeting.

May 13, 2014, 8:34 p.m.

Interested in how other labs celebrate accomplishments and have fun outside the lab? We've interviewed 3 PIs to learn about how their labs celebrate.

May 8, 2014, 3:07 p.m.

New fluorescence resonance energy transfer (FRET)-based calcium biosensors and FRET-based sensors for the intracellular imaging of transition metal ions.

May 6, 2014, 1:07 p.m.

Addgene explains what a material transfer agreement is, how it can benefit you and your academic institution, and how Addgene makes MTAs easy.

May 1, 2014, 8:23 p.m.

Advice on starting a lab from three PIs who have successfully built their own academic labs.

April 29, 2014, 1:43 p.m.

Addgene answers questions about lentiviral plasmids, including 2nd vs 3rd generation transfer vectors, packaging plasmids, biosafety concerns, and more.

April 23, 2014, 1:08 p.m.

Tips for how to choose a research project in a life science lab.

April 17, 2014, 2:45 p.m.

Tips for being more environmentally friendly in a life science research lab.

April 15, 2014, 3:11 p.m.

Advice for scientists choosing a lab, including interviews with Principal Investigators and important factors to consider in your decision.

April 8, 2014, 2:50 p.m.

The next post in the Plasmids 101 series focuses on the promoter region, the element responsible for initiating the transcription of your insert into RNA.

April 3, 2014, 8:05 p.m.

The first in a series of blog posts in which PIs give advice about achieving success in academia, choosing you research topic, and more.

April 1, 2014, 5:33 p.m.

In vivo imaging is used to study individual plasmids or protein-protein interactions in organs and whole mammals.

March 27, 2014, 5:14 p.m.

Read about how mammalian plasmids differ from their bacterial counterparts, including how replication occurs and whether selection is necessary for transfected cells.

March 25, 2014, 3:15 p.m.

Ten tips to help scientists negotiate their work/life balance from Joanne Kamens, Addgene's Executive Director.

March 20, 2014, 3:04 p.m.

Dr. Le Cong, first author on a seminal CRISPR article from Dr. Feng Zhang's lab, answers FAQs about the genome editing technology.

March 13, 2014, 4:08 p.m.

CRISPR-Cas9 technology appears in numerous publications, including its use for genome editing in monkeys and x-ray crystallography of Cas9's 3-D shape.

March 11, 2014, 5:55 p.m.

Tips for making the most of your mentoring relationship, including the ebook "Mentoring for Scientists".

March 6, 2014, 7:12 p.m.

Interview with Ed Boyden discussing his newest optogenetics tools and the questions that motivate neuroengineering.

March 4, 2014, 4:49 p.m.

This post discusses the core replication and resistance features of yeast vectors and explains how these plasmids differ from their bacterial counterparts.

Feb. 25, 2014, 7:11 p.m.

Various lentiviral CRISPR-Cas9 libraries have been developed to perform genome-wide knockout screens, including the GeCKO library from Feng Zhang's lab.

Feb. 20, 2014, 4:57 p.m.

The advantages of participating in a peer mentoring group for scientists and how to form your own.

Feb. 18, 2014, 4:22 p.m.

Tips for naming your plasmid in a way that is clear and informative to you and your colleagues.

Feb. 13, 2014, 1 p.m.

Five steps to help graduate students and postdocs write their first grant proposal.

Feb. 11, 2014, 12:13 p.m.

The third post in the mentoring series, this post describes ways you can approach finding a science mentor and advice on how to “make the ask.”

Feb. 4, 2014, 3:50 p.m.

Learn about the importance of antibiotic resistance genes in plasmids. How they work, how to use them, and some great tips.

Jan. 30, 2014, 3:29 p.m.

Use these protocols and tips to make your own competent cells and transform your DNA. It's easy and affordable.

Jan. 28, 2014, 3:10 p.m.

GreenGate is a cloning system for rapidly assembling plant transformation constructs and is based on the Golden Gate method.

Jan. 23, 2014, 3:23 p.m.

Identify which extracurricular activities will help you gain the skills you need to succeed in your science career.

Jan. 21, 2014, 3:08 p.m.

Advice for choosing an advisor for a PhD or postdoctoral lab. Second in a series on Mentoring for Scientists.

Jan. 16, 2014, 2:56 p.m.

An inside look at the ten most frequently distributed plasmid technologies at Addgene from the past ten years.

Jan. 8, 2014, 3:10 p.m.

Three useful tips to help you organize your lab notebooks, keep track of tasks, and share protocols with other lab members.

Jan. 2, 2014, 3:44 p.m.