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We narrowed to 610 results for: ACE;

Showing: 361 - 380 of 610 results

  1. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...required for a mini amplification reaction to take place.  When the PCR is complete, a droplet reader extracts...contamination. Optimize your PCR For a good starting place on what PCR parameters to use, see Lock’s seminal...

  2. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...labeling so that a cell’s projections are easy to trace and not lost in a jumble of fluorescent neurons.... But notice in figure 4b how difficult it is to trace individual cell’s axons, particularly the two green...

  3. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...samples accurately and in a way that can be easily traced. If you’re just starting a lab, or are looking ...barcode can store a lot of information in a small space and is ideal for tracking large collections. Many...

  4. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ... and vary in shape and structure. Their porous surface provides a large area for binding, but antibodies... than agarose beads. Proteins conjugate on the surface of magnetic beads, and are therefore completely...

  6. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...through the stomata into the abaxial site (lower surface) of the leaf. This is a simple and easy technique...Mortensen et al., 2019): Leaves or whole plants are placed in an Agrobacterium solution and a vacuum is applied...

  8. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...struggling to understand how the researchers decided to place their gates on the plots! Flow data is messy, so...., Pons, J. A., & Muro, M. (2018). In vitro intracellular IFNγ, IL-17 and IL-10 producing T cells correlates...

  9. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...commercial antibodies can be tethered on the viral surface, expanding the range of targetable cell types. ...et al. (2023). Time-tagged ticker tapes for intracellular recordings. Nature Biotechnology, 41(5), 631...

  10. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...preparations are purified by iodixanol gradient ultracentrifugation and concentrated to purity and titers adequate...maintained for ~20 passages before being discarded and replaced with a new vial of early passage cells. Approximately...

  11. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...without disrupting plasmid representation. A good place to start is with a protocol for a library of similar...Moderated by the Feng Zhang lab, this is a useful place to ask experimental questions.  Practical Considerations...

  13. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...across from the abasic site before filling the empty space. Over several rounds of rational mutagenesis, the...editors Excises G, T, or C, respectively, to be replaced with another base. References Chen, L., Hong...

  14. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...are 27 incompatibility (Inc) groups in the Enterobacteriaceae family alone, and this is expanding as we...expelled antibiotic resistant plasmids from Enterobacteriaceae family members within the mouse gut (Kamruzzaman...

  15. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ... or the statistical variation is calculated and placed in the legend. Widely reported difficulties in ...on the fly”. This becomes intuitive with touch interfaces where the viewer is more likely to want to manipulate...

  16. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...expressed on T cells to make CAR T cells. The extracellular scFv portion of the receptors recognizes an ...an antigen of interest while the intracellular T cell receptor portion of the CAR facilitates signal transduction...

  17. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...structural complexity inside the cell or on its surface. Bear in mind, the voltage setting of the laser...through conjugated antibodies targeting the cell surface markers CD11 (anti-mouse CD11b-BV510), which is...

  18. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    Published
    Nov. 25, 2015, 4 p.m.
    ..., their makeup (in terms of nationality, gender, race, etc), or their career outcomes. Some of the more...their mentorship with the same critical eye they place on others within their labs - as a sort of over-...

  20. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...but is activated by GAL4 binding to UAS. If you place the GAL4 gene downstream of a tissue- or developmental...inhibiting the binding of GAL4 to UAS. One can therefore place GAL4 and GAL80 under different promoters and create...
Showing: 361 - 380 of 610 results