...significant advances in the understanding and application of CRISPR/Cas9 technology are coming along at...of Chase L. Beisel and Rodolphe Barrangou, had applied the CRISPR/Cas9 system to developing an antibiotic...feature when he said, “All of this has basically happened in a year. It’s incredible.” Looking for CRISPR...

...Career and skills development can happen anywhere, whether you realize it or not. In the lab, you could...don’t think you’re qualified. The worst that can happen is they say no, which is infinitely better than...good, customized letter to the place that you are applying.” - Melina Fan, Chief Scientific Officer If there...

... The new tool was developed and implemented by applying the Golden Gate cloning method to human proteins...says the new tool might even find therapeutic application in addition to their use in basic biology, for... make the library even better, we would be very happy to share updated versions of the kit.” Reference...

...Academic Job Interview by Erik Snapp Addgene depositor and guest blogger Erik Snapp breaks down how to prepare....org.  Thank you again for a fantastic year and happy reading. Best wishes on your research in 2020! ...

... huge, there are times when a more incremental approach is prudent. What's in the CRISPR kinome library...already have Cas9). Each library consists of approximately 3,000 gRNAs, as opposed to the 70,000 or more...community will find these reagents to be useful. Happy screening! Many thanks to our guest blogger, John...

...ve also carved pumpkins (yes, Blugene made an appearance there too) and shared fascinatingly creepy science...defective exoskeleton formations. These genes were appropriately named as spook, spookier, phantom, disembodied... grasshopper harbouring hairworm: a proteomics approach." Proceedings of the Royal Society B: Biological...

...need for a faster and higher-throughput in vitro approach. Using data from The Cancer Genome Atlas (TCGA...and fusions represented in this collection, approximately 80% (over 800) of them had not yet been functionally... viability compared to a wild-type control. Approximately ~22% of the mutations, however, significantly...

...Since the first research applications of GFP were published in the 1990s, biologists have spent a lot... Sattarzadeh et al., Sci Rep. 2015 CC BY 4.0 Applications and limitations of GFP photoconversion One concern...photoconverted, so this technique may not be appropriate for high-pH cellular compartments. Sattarzadeh...

...to explore the paired double-strand break (DSB) approach at a variety of loci. We were pleasantly surprised... size and frequency [3]. There are several applications in which the production of deletions by paired... RT-qPCR, or immunoblotting to detect. Another approach is to target two exonic sites within a single ...

...were willing and able to listen to Addgene’s new approach of working with the industry-based scientific ...reduced to days and weeks. As of June 2020, we are happy to have the support of the following organizations...project is complete, Ginkgo will have synthesized approximately 3,000 constructs to support SARS-CoV-2 research...

... value and priority put on good mentoring and applauded the new requirement for PIs to include in their... of funding basic research at NEB and got some applause when he talked about how he boycotts publishing...opportunities.  Scientists must do science to be happy so the beginnings of solutions can’t come too soon...

... if there are forces acting on them. For the application we were looking at, magnetic isolation of circulating...out the cell capture system.) Cancer and other applications Addgene: What does this technology mean in the...expression level. Labs have been trying different approaches to improve CTC capture, but I hope that our antibody...

... al., 2019. Applications for SPARC SPARC technology is versatile and can be applied across cell types...population, it’s easier to visualize individual/non-overlapping cells. While transgenic animals are commonly ...

...inflammation, and neurotransmission. But with approximately 950 different human genes encoding GPCRs (Takeda...GPCR of interest, a Gα-Rluc8 plasmid, and the appropriate Gγ-GFP2 and Gβ constructs. Cells are then treated...plasmids in a number of different experimental approaches. They first assessed the activation of different...

...go-to method for mapping where a protein binds genome-wide, and has been widely applied in many model organisms... the chance of false-positives, as proteins may appear to be binding at particular genomic sites when ...

...aggregation, it’s recommended for most imaging applications. What can you do with SunTag? Tanenbaum et al...flames,” it’s clear that the system is broadly applicable across many areas of research. Beyond fluorescence...activation of target genes, but we’ll save that application for another day! References 1. Tanenbaum, Marvin...

...been requested 100+ times Since we so sincerely appreciate our depositor’s dedication to scientific sharing...plasmids with us. One way we can gauge if people are happy depositors is if they deposit plasmids more than...off! To share your photo, tag @Addgene and #happydepositor on Twitter. All Blue Flames started with a ...

...called protospacers. Repeats: Bacterial DNA that appears between each spacer in the CRISPR array, distinguishing... are recognized by the Cas9 protein from the appropriate species. Some Cas proteins, like LbCas12a or ...simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer to the programmable...

...as #happydepositors who stopped by to say hello. Needless to say we all got very good at applying temporary...six continents who dropped by the booth. We were happy to chat with lots of researchers who weren’t aware...

... though not all, of these defenses. For some applications of genome engineering - such as certain forms...and improved SB transposase, SB100X, that is approximately 100-fold more efficient than the first-generation...Epub 2009 May 3. PubMed. 3. Singh H, et al. A new approach to gene therapy using Sleeping Beauty to genetically...