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...offer tools to help with these decisions.  So when do you want to codon optimize your gene of interest?...useful in expressing functional proteins in hosts that do not naturally express that gene. The protein you ...

...expression. To design a viral vector system that could do both, Addgene depositor Didier Trono and collaborators... lentiviral system (Figure 2). The HIV genes that do remain are very important for viral production: Gag...

...Gene X in the untreated cells—but can we be certain? Do we know whether the plasmid delivered into the cells...negative controls that produce a similar product, but do not target an endogenous gene in your experimental...

...of necessary data requirements and likely will not do so (calling it a “sisyphean task” given the endless...Firstly, we rely on many other labs to teach us how to do experiments and understand their data. Likewise, ...

... Michael Früh submission, which we have entitled "Do You See What I See?" I was bored this weekend while...

...other aspects of molecular biology. “What I'd like to do with the plasmids from Addgene is have the students...

...quantification so that you can focus on what you want to do with your plasmid without spending time and resources...

...expect given the excitement surrounding anything to do with CRISPR, our most popular post in July (with ...

...wild-type and engineered variants of Cas nucleases. In doing so, they also created a modular toolkit for Cas-...

...all the excitement and speculation, we thought we’d do a little digging and assemble a rough guide to the...

...editor. Cas13 enzymes are advantageous because they do not require a PAM, and RNA targeting is potentially...flanking sequence (PFS) adjacent to the target, but many do not, showing the flexibility of this system.  As ...

...array of base editing reporters that can help you do just that. Monitoring APOBEC and Cas9-mediated editing...tested their hypothesis with GFP fluorescence. To do this they generated a silent GFP construct, that ...

...tool that is easy to use with other plasmids.  How do you know if your plasmids are incompatible? Scientists...will be based on sequence data. However, if these do not provide clarity, a wet lab transformation experiment...

...Expression in Mammals AAV Titers: Where do they come from and what do they mean? A Match Made in Heaven: CRISPR...

... using site-directed mutagenesis of Spinach. In doing so, they identified the nucleotides that contribute...Just as vegetables come in a variety of colors, so do aptamers! Corn was recently described in 2017 (Song...

...being published on our own topic. Only on occasion do we then have time to read about research from other...otherwise never learn from the valuable research we are doing. Browse the site, learn from others, share ideas...

...sites, or you can utilise several online tools that do it for you. One such option is the CRISPR Design ... take roughly 3 months from the day you decide to do a gene modification experiment in mice to the day...

...guest blogger Derek Simon. The actual experiments you do will be determined by the topic you’re interested...Second, this may sound rudimentary, but before you do any type of behavior analysis, simply take a few ...

...Education & Tools menu Further down What happens if you do scroll down? You’ll find Blugene, as well as information...

...yourself, you’re probably wondering: What does it do and how does it work? LentiCRISPR v2 purpose As its...