We narrowed to 379 results for: ENA;

1. Plasmids 101: Repressible Promoters

   Type

   Blog Post

   Published

   Dec. 20, 2022, 2:15 p.m.

   ...ethanol accumulates, it binds to the repressor, enabling it to bind the promoter and repress pADH1 activity...

2. CRISPR 101: Off-Target Effects

   Type

   Blog Post

   Published

   Feb. 8, 2024, 2:15 p.m.

   ...nothing to worry about all. Let’s consider several scenarios below for a CRISPR experiment which involves making...

3. PiggyBac-ing Through the Genome Editing Field

   Type

   Blog Post

   Published

   May 31, 2016, 3:30 p.m.

   ...technology, however, is that the transposase also enables the excision of the transposon in a completely ...

4. Finding Your Perfect Job After University

   Type

   Blog Post

   Published

   Jan. 12, 2016, 3:30 p.m.

   ...and how I felt about the different positions to enable them to decide for themselves.  Here is my experience...

5. Building Global Connections with the International Mentorship Program USA-EUROPE

   Type

   Blog Post

   Published

   Nov. 6, 2015, 3:30 p.m.

   ...emphasize in order to be selected in foreign labs.” Elena Grosso (Mentee IMP 2014-15, University of Valencia...

6. 9 tips for a successful postdoctoral experience

   Type

   Blog Post

   Published

   Oct. 9, 2018, 12:21 p.m.

   ...? What are your controls? Will these controls enable you to interpret your results? Will the experiment...

7. Important Considerations When Using AAVs

   Type

   Blog Post

   Published

   June 13, 2017, 2:30 p.m.

   .... The Future What new types of research may be enabled by the use of AAVs? 1) Combined functional and ...

8. Quick Guide to All Things Lentivirus

   Type

   Blog Post

   Published

   March 21, 2017, 2:30 p.m.

   ... the Tet-on system, tTA has been modified (and renamed rtTA) to bind TRE only in presence of Dox. In this...

9. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

   Type

   Blog Post

   Published

   Oct. 8, 2020, 1:15 p.m.

   ...should be made available for cell culturing and maintenance of the cell lines. Providing such an environment...

10. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type

    Blog Post

    Published

    Nov. 2, 2021, 1:15 p.m.

    ...systematically test the potential causes of these phenomena by analyzing the plasmid and viral DNA sequences...

11. A Guide to Getting Started in Undergrad Research

    Type

    Blog Post

    Published

    June 8, 2021, 1:15 p.m.

    ...: managerial duties ex: order materials, lab maintenance Administrative assistant: assists PI with admin...

12. Sequencing Options for CRISPR Genotyping

    Type

    Blog Post

    Published

    Oct. 4, 2016, 2:30 p.m.

    ...PMC3782611. 8. Tsai, Shengdar Q., et al. "GUIDE-seq enables genome-wide profiling of off-target cleavage by...

13. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type

    Blog Post

    Published

    June 28, 2018, 3:01 p.m.

    ... Efficient CRISPR/Cas9-assisted gene targeting enables rapid and precise genetic manipulation of mammalian...

14. Antibodies 101: Introduction to Antibodies

    Type

    Blog Post

    Published

    Jan. 19, 2021, 2:15 p.m.

    ....bios.2016.04.091 Cruse JM, Lewis RE, Wang H, Geziena MT, Steven GE, Lorna J. (2004) ANTIGENS, IMMUNOGENS...

15. Antibodies 101: Flow Cytometry

    Type

    Blog Post

    Published

    July 20, 2021, 1:15 p.m.

    ...methanol, fix and permeabilize in a single step by denaturing proteins and dissolving lipids including those...

16. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type

    Blog Post

    Published

    Sept. 10, 2015, 2:30 p.m.

    ...mouse line itself. Generation, breeding, and maintenance of a mouse line is also an expensive and time-consuming...

17. Selecting Your Plasmid Purification Kit

    Type

    Blog Post

    Published

    May 27, 2025, 1:15 p.m.

    ...automation-friendly kits and/or automation systems that can enable you to run thousands of reactions with relatively...

18. CRISPR 101: Cytosine and Adenine Base Editors

    Type

    Blog Post

    Published

    Feb. 13, 2025, 2:15 p.m.

    ...K. (2020). A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing. Nature...

19. Troubleshooting and Optimizing a Western Blot

    Type

    Blog Post

    Published

    Sept. 17, 2024, 1:15 p.m.

    ...protease inhibitors to lysate Insufficient denaturing conditions Adjust lysis conditions: time, temperature...