...leadership. Perhaps one of your advisors or mentors has urged you to work on developing your “leadership ...just have to know it when you see it? “The Three Stages of Your Career: Doer, Manager, Leader” by Charlotte...importance of these very different roles. At any stage in our careers we may be in a cycle of doing, managing...

...in state machine technology? Biological research has produced a massive amount of information regarding...two-input gene regulation programs. The software has two components - a database of pre-compiled register...reporter-based) each come with their advantages and disadvantages. Sanger sequencing is simple and the...

...possible angle to leave no stone unturned, but cancer has been producing landslide after landslide, only adding...used immunotherapeutic agents. Currently the FDA has 12 approved monoclonal antibodies targeting the PD-L1...bacteria, the tumor cells that escape the elimination stage acquire genetic alterations and alter cell-surface..., by way of favorably attaining the equilibrium stage. This can be called “stalemate equilibrium” where...the acute immune responses that eliminate early stage cancer cells, chronic inflammatory responses can...

...Addgenies what their favorite career tip is, or what has helped them the most. Take a look below! In the lab...organization/position exist yet?", you are in the early stages of creating an opportunity. That's how Addgene ...

... virulence (Tollefson et al., 1996) – cell death has been reported when using vectors, and immune inactivation...the infection to sometimes result in cell death, has led to interest in using adenoviral vectors for vaccine...death protein (E3-11.6K) is required at very late stages of infection for efficient cell lysis and release...

...rather than for setting up your initial voltages and gates. Ashlyn has a research background in CD8+ T cells...color controls are used to help you set up your voltages and can also be helpful for compensation. You ... cells. This can be achieved by adjusting your voltage using your single color controls (Figure 1A), instead... using beads and not experimental samples, the voltages and compensation you set from your single color...

...high for both plasmids, so each plasmid “thinks” it has a higher copy number than it actually does, and neither...large, low copy virulence plasmids. This strategy has been successful in displacing, or “curing” the virulence...Using plasmid incompatibility for therapeutic advantage Although it may seem like plasmid incompatibility...

...FLP efficient The first version of FLP discovered has a temperature optimum of 30 °C and is therefore inefficient...evolution led to the identification of FLPe which has a temperature optimum of 37 °C (Buchholz, Angrand...Properties of FLP Recombinase Evolved by Cycling Mutagenesis.” Nature biotechnology 16(7): 657–62. PubMed ...

...particles, the functional infection unit. Each particle has a viral envelope, which fuses with the target cell...the fear of random genomic integration causing mutagenesis in the host. Adenoviruses can deliver relatively...non-integrating and infect non-dividing cells. AAVs primary advantage over adenovirus is their low immunogenicity, which...Dividing Cells Moderate Immunogenicity Insertional Mutagenesis Possible Lentivirus Infects non-dividing...non-dividing cells Insertional Mutagenesis Possible Adenovirus Remains Episomal (Little Risk of...of Insertional Mutagenesis) High Gene Expression Infects Non-Dividing Cells Large Packaging Capacity (... AAV Remains Episomal (Little Risk of Mutagenesis) Very Low Immunogenicity Infects Non-Dividing...

...applicable in DSB-induced genome engineering, it has biological importance for the repair of breaks that...directed repair and genome engineering CRISPR-Cas9 has revolutionized the genome engineering world and made... chromosomal aberrations. Pfeiffer P, et al. Mutagenesis (2000). PubMed. Multiple Pathways of Recombination...

...bridge helix. Created with BioRender.com.   Cas9 has two major lobes, called NUC (for nuclease) and REC...gray). Target DNA strand, light gray.   Once it has found a PAM site, Cas9 needs to check for complementarity...orange) and HNH (red) domains, while the REC lobe has been hidden for visibility. A RuvC loop caps the ... and different techniques all bring their own advantages—and blind spots—to these questions. Each new ...

...emerging validation technology. Digital PCR has several advantages over mismatch nuclease assays that will...As mentioned, these assays have several major advantages over the commonly employed mismatch nuclease ...bi-allelic mutations: Perhaps the most noteworthy advantage of digital PCR assays is that they are easily .... 6. Fu, Y. et al. High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells...

...senior mentors available to go around. My advice has been to find 5 interested peers and form your own...step-by-step, how-to guide to get one started. Advantages of the group mentoring format Adding peer advice...Because the participants are at a similar development stage, they have a lot to learn from each other. Some ...the group. The group can be one gender and career stage (e.g. all women, all men, all postdocs, all grad...support each other as you transition to the next stage in your careers! Stay in touch…this group will be...

...quantified at 595 nm. This method is time consuming and has low sensitivity so it’s not used much. However, the...concentration. It’s important to consider the advantages and disadvantages of each method and when one method might...

...the most widely used CRISPR nuclease, recent work has shown that alternative enzymes may better suit specific...Joung and Jennifer Doudna Labs used targeted mutagenesis to generate hyper accurate Cas9 (HypaCas9). This... sites. Using a combined random and directed mutagenesis approach, the Anna Cereseto Lab created pX-evoCas9...experimental approaches, knowledge of both the advantages and limitations of each Cas enzyme can help determine...

...since its development, CRISPR/Cas9 genome editing has been used to study the effect of gene knockout in...-target effects and/or toxicity, it would be advantageous to instead use a preexisting, conserved cellular...leading nucleotide could be processed, a distinct advantage over other methods that favor certain nucleotides...potential of PTGs. Applications of PTGs The major advantage of the PTG system is its universality; robust ...

...Collin’s Lab in 2009. From 2009 to today, Sample6 has changed focus and names, swapped leadership, and ...of technologies, effectively communicating the advantages embodied, and driving the enterprise value of...plan competition and advance investors to the next stage of diligence." In business competitions everything... be OK with the constant fear of failure at all stages in the startup". To gain insight into "the random-walk...

...?” Some of the Tweeted comments: “A good mentor has your goals/needs/situation in mind, rather than (...being coddling, to listen without being taken advantage of, and to respect my students as developing colleagues...

...PAM motif, averaging 19 targets per gene. The lab has previously used the library with SpdCas9 to perform...lab discovered a new Cas protein, CasX, from metagenomic analysis of microbial DNA from groundwater. Now...

...with chemicals in it,” leaving me to wonder how he has survived this long without downing any dihydrogen...graduate with my BSc in Anatomy, the University of Otago had established the Centre for Science communication...Department of Science Communication at the University of Otago. Her research focuses on misinformation about sexual...