We narrowed to 452 results for: HA tag

...might be more successful. “Shock and kill” has the advantage of killing cells infected with HIV-1 to deplete...tough job! Kamel Khalili’s lab at Temple University has demonstrated two potential strategies for CRISPR-... treatment with CRISPR/Cas9 SAM increased the percentage of cells with activated HIV-1, measured via HIV...

...characterization of over 1,000 mutations, and the group has deposited several hundred plasmids from this study...lentiviral constructs using the High-Throughput Mutagenesis and Molecular Barcoding (HiTMMoB) technique (... any mutations that that confer proliferation advantages will be revealed in the absence of growth factor...

...fact, they’ve been following a set of clues that has led them to their biggest discovery yet: Addgene ...submit your name suggestions here or by using the hashtag #AntibodyMakesThree on Twitter. As with Blugene...

...transsynaptic spread is useful for neuronal tracing, it has two practical limitations. First, these high levels...projection neurons). Research into these phenomenon has shown that the RABV G protein is necessary and sufficient...monosynaptically from these cells. These modifications take advantage of the fact that RABV is an enveloped virus. In...

...Request  has seen a fantastic response from the research community. Customers have taken advantage of the...

...multiple sites in cultured human cells. While it has not yet been demonstrated, they say the vectors are...in June. “The multiplexity is one of the most advantageous properties of CRISPR/Cas9 compared to ZFNs and...

... disrupting ribosome-mRNA complexes. Recent work has adapted this technique to monitor local translation...contains 24 copies of SunTag, a synthetic scaffold that can recruit GFP fused to the SunTag-specific antibody...Imaging by Coat protein Knock-off) instead takes advantage of the ribosome’s movement. When the ribosome ...indicating translational repression. During later stages, single RFP-labeled osk and Oskar protein were ...scFv. Each time the reporter is translated, the SunTag sites on the nascent polypeptide recruit scFV-GFP..., as the authors of these papers did. Since the SunTag-based system tethers mRNAs to the plasma membrane...Additional Resources the Addgene Blog Learn More about SunTag Catch up on Your GFP Background Choose the Brightest...

... AAV quality control Historically, AAV vector QC has been sparse including only titration of AAV particles... used SMRT sequencing, TA-based ligation and tagmentation-based methods for AAV genome sequencing (Tai...self-complementary AAVs and the TA-based ligation and tagmentation preparation approaches described previously ...single-stranded and both TA-based ligation and tagmentation require a double-stranded template.  Using viral...the mix to serve as effective substrates for tagmentation-based library preparation procedures. This finding...packaging plasmids. We decided to use this to our advantage by designing a Python script that looks for pre-defined...

...requires. Using the (Last name, Year) format also has the benefit of exposing you to relevant researchers...just gotta do it." At the end of the day, someone has to write the article and that someone is going to...retreat to the woods for months at a time, so take advantage of whatever time you have. If you’re like me, ...

...native organism.” This is possibly a better idea and has been used successfully in the past (Angov et al.,...functionally analyze the protein produced to ensure that it has folded properly. You may be able to avoid getting...normally found at low abundance in E. coli. The advantage of producing additional tRNAs is that you can ...

...leadership. Perhaps one of your advisors or mentors has urged you to work on developing your “leadership ...just have to know it when you see it? “The Three Stages of Your Career: Doer, Manager, Leader” by Charlotte...importance of these very different roles. At any stage in our careers we may be in a cycle of doing, managing...

...in state machine technology? Biological research has produced a massive amount of information regarding...two-input gene regulation programs. The software has two components - a database of pre-compiled register...reporter-based) each come with their advantages and disadvantages. Sanger sequencing is simple and the...

...possible angle to leave no stone unturned, but cancer has been producing landslide after landslide, only adding...used immunotherapeutic agents. Currently the FDA has 12 approved monoclonal antibodies targeting the PD-L1...bacteria, the tumor cells that escape the elimination stage acquire genetic alterations and alter cell-surface..., by way of favorably attaining the equilibrium stage. This can be called “stalemate equilibrium” where...the acute immune responses that eliminate early stage cancer cells, chronic inflammatory responses can...

...Addgenies what their favorite career tip is, or what has helped them the most. Take a look below! In the lab...organization/position exist yet?", you are in the early stages of creating an opportunity. That's how Addgene ...

... virulence (Tollefson et al., 1996) – cell death has been reported when using vectors, and immune inactivation...the infection to sometimes result in cell death, has led to interest in using adenoviral vectors for vaccine...death protein (E3-11.6K) is required at very late stages of infection for efficient cell lysis and release...

...rather than for setting up your initial voltages and gates. Ashlyn has a research background in CD8+ T cells...color controls are used to help you set up your voltages and can also be helpful for compensation. You ... cells. This can be achieved by adjusting your voltage using your single color controls (Figure 1A), instead... using beads and not experimental samples, the voltages and compensation you set from your single color...

...high for both plasmids, so each plasmid “thinks” it has a higher copy number than it actually does, and neither...large, low copy virulence plasmids. This strategy has been successful in displacing, or “curing” the virulence...Using plasmid incompatibility for therapeutic advantage Although it may seem like plasmid incompatibility...

...FLP efficient The first version of FLP discovered has a temperature optimum of 30 °C and is therefore inefficient...evolution led to the identification of FLPe which has a temperature optimum of 37 °C (Buchholz, Angrand...Properties of FLP Recombinase Evolved by Cycling Mutagenesis.” Nature biotechnology 16(7): 657–62. PubMed ...

...particles, the functional infection unit. Each particle has a viral envelope, which fuses with the target cell...the fear of random genomic integration causing mutagenesis in the host. Adenoviruses can deliver relatively...non-integrating and infect non-dividing cells. AAVs primary advantage over adenovirus is their low immunogenicity, which...Dividing Cells Moderate Immunogenicity Insertional Mutagenesis Possible Lentivirus Infects non-dividing...non-dividing cells Insertional Mutagenesis Possible Adenovirus Remains Episomal (Little Risk of...of Insertional Mutagenesis) High Gene Expression Infects Non-Dividing Cells Large Packaging Capacity (... AAV Remains Episomal (Little Risk of Mutagenesis) Very Low Immunogenicity Infects Non-Dividing...

...applicable in DSB-induced genome engineering, it has biological importance for the repair of breaks that...directed repair and genome engineering CRISPR-Cas9 has revolutionized the genome engineering world and made... chromosomal aberrations. Pfeiffer P, et al. Mutagenesis (2000). PubMed. Multiple Pathways of Recombination...