We narrowed to 853 results for: IGH@

...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...Chemistry for his contributions to super-resolution light microscopy, comes from his recent Nature Communications...difficult to study in living cells using conventional light microscopy due to their small size (<250 nm), and...probes had to target endosomes, be exceptionally bright (which is necessary for effective STED), and be...

.... The protein of interest is then eluted using a high imidazole buffer that outcompetes the interaction...protein but not that of the protein of interest. A high imidazole elution buffer disrupts the interaction...concentration of imidazole used in the wash buffer should be high enough to disrupt contaminant-metal ion binding ...therefore more selective. When using systems with higher levels of histidine-containing protein contamination... histidine residues that are present, the more tightly the protein will bind to the metal ion substrate...

...conference you’re attending and who approaches you. You might also have cross-team projects within your own lab... errors in our LIMS tracking system. Though you might not have to communicate with a team of software ...document, I update the training frequently. In hindsight, getting a list of frequently used words like ...everyone on the same page. Others in the meeting might also have the same question and asking can help ..., meetings will be shorter and more productive. Higher Productivity. Taking active measures in improving...

..., these problems can be overcome by screening a higher number of potential clones. Golden Gate assembly...constructs with an attB recombination scar encoding eight amino acids, but Golden Gate assembly can be designed...that may not be amenable to "domestication", you might want to consider using an alternative method like... for cloning complicated constructs in a single, high-efficiency step. References: Sequential amplification...one pot, one step, precision cloning method with high throughput capability. Engler C, Kandzia R, Marillonnet...

...cell lines to the -omics data. The cell lines with higher expression of the target protein, as determined...expression, are often preferred for this approach. A highly selective antibody should be able to detect even...only be able to detect the target protein at the high levels caused by overexpression. Additionally, if...clones The antibody validation process represents a high level of effort and time, which may not be available... & ABIF Consortium. (2024). A guide to selecting high-performing antibodies for Synaptotagmin-1 (Uniprot....15467 Virk, H. S., Rekas, M. Z., Biddle, M. S., Wright, A. K. A., Sousa, J., Weston, C. A., Chachi, L....

...of the ABE8s ranged from 1.5x higher for positions A5-A7 to 3.2x higher for positions A3-A4 and A8-A10...synthetic library of TadA sequences to create the eighth generation of adenine base editors, called ABE8... in variants named “ABE8.x”: “8” stands for the eighth round of ABE evolution and the “x” is the placeholder...editing frequencies. For applications that require high DNA editing specificity, for example for potential...

...of unionization continues to be a polarizing one. High-profile universities remain outspoken in their opposition...FLSA changes, including finding money in already tight lab budgets and even simply being able to categorize...salary perspective, while this is a move in the right direction, the increase notably still does not meet...Workshop 2016 At past symposia, FoR worked to highlight some of the problems facing postdocs and graduate...

...these high levels of viral replication are toxic. Second, infecting subsequently higher and higher order...transmission occur, spreading the virus through higher and higher order neural connections. While this transsynaptic...

...expression and degradation. These workshops not only highlighted new tools but also included time for questions...presented at the C. elegans meeting. Here I’ll highlight a few toolkits that we have available at Addgene..., and Erik M. Jorgensen. "SapTrap, a toolkit for high-throughput CRISPR/Cas9 gene modification in Caenorhabditis...

...above, or other. High or Low Copy Choose from: high or low copy. Choose “high copy” if a sufficient...standard cloning strain DH5α. For plasmids with highly repetitive sequences (prone to recombination in...Example of the deposit spreadsheet showing the first eight columns and a pop-up instruction box for the “Purpose...

...Updated Apr 6, 2021. If you’re doing high throughput work, it’s likely that you come into the lab wishing...transformation One protocol that has been automated is high throughput transformation. With our ever growing...the deck of the Biomek. The FXP id also able to do high throughput DNA preps and other molecular work if... through this process. With enough agar and the right program, the liquid handler can fill 400 vials in...hand. How can you start automating your lab? You might not be doing enough transformations, minipreps, ...

...possible by the use of our high-throughput DNA isolation process that yields high-quality isolated DNA samples... on our ability to provide requesting scientists high quality data! Addgene's new in-house NGS process...be familiar with our efforts to ensure access to high quality, ready-to-use viral preps and pooled libraries...Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD, Sanjana NE, Zhang F (2017) Genome-scale CRISPR-Cas9...

...installment of the Early Career Researcher Toolbox will highlight four tools for finding journal articles related...summary of the topic and a chronological list of highly cited papers that contain your search term. When...of the page, as well as the number of citations highly influenced by this paper. Influence is measured...number of publications that heavily cite, or are highly influenced by the author. You can also see who ...

..., and so on and so forth. This also serves as a light technical control - an RQ value of 1.5 means you...quencher to quantify specific amplicons.  Sybr has high background noise, due to the large amount of dsDNA...DNA and the length of the amplicon and it has a higher rate of false positives (Colborn, 2008; Harridan...number of factors will determine which approach is right for your experiment. You’ll have to consider:  ...analysis of copy number variants based on real-time LightCycler PCR. Curr Protoc Hum Genet. 2014;80:7.21.1-7.21...

... vivo CRISPR screens. AAV vectors can be made at high titers equal to those achieved with lentivirus but...less involved setup when working with mice and slightly less red tape for approval processes. Additionally... means you can customize your CRISPR screen to a high degree. This is particularly helpful when investigating...Breakefield, X. O., Maguire, C. A., & György, B. (2019). High levels of AAV vector integration into CRISPR-induced...screening by AAV episome-sequencing (CrAAVe-seq) is a highly scalable cell type-specificin vivoscreening platform...

...chains and two small light chains that stick out to form the “arms” holding the highly variable regions that...membrane proteins that may unfold due to camelid high body temperature (37.2 - 39.2°C). The Seeger lab...References Geertsma ER (2013) FX Cloning: A Versatile High-Throughput Cloning System for Characterization of...resources on the Addgene blog Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification...

...cells to grow and divide only at the right times and in the right places. Checks and balances: protecting...proteins naturally lack areas that are accessible, high-affinity binding targets for potential therapeutic...therapeutic agents. Nevertheless, a glimmer of light remains for this elusive target. Recent improvements in ...results and customize the small molecules for a higher affinity fit. Kevan Shokat’s group at the University...

...quality. To make sure our AAV vectors are of the highest quality, we undertake a rigorous quality control...vectors, and helper plasmids (Chadeuf et al., 2005, Wright et al., 2011). In fact, the majority of hits come...samples from 0.1-20% and discovered that, with a high enough sequencing depth, we could detect contamination...with exquisite detail (Lecomte et al., 2015). This higher level of quality control is recommended but not...Acids 4:e260 . https://doi.org/10.1038/mtna.2015.32 Wright JF, Zelenaia O (2011) Vector Characterization Methods...

...Addgene we've set out to fill this gap by both highlighting that researchers do learn MANY transferable ...the lab and by offering advice on areas where you might need some help. Today in our transferable skills...you will have a much better chance of creating a high-performing team - which is every manager’s dream... a weekly basis! The silence and maybe even the slight awkwardness can spur people to speak up. Once aware...

...using a high-speed ultracentrifuge. During the spin, the virions will form a band due to their high density...nationwide where you can obtain excellent quality, high titer rAAV (including Addgene!), but you can also...terminal repeats (ITR). You should always have a high-quality plasmid standard and rAAV reference material...Zen, Z., Espinoza, Y., Bleu, T., Sommer, J. M., & Wright, J. F. (2004). Infectious titer assay for adeno-associated...