We narrowed to 708 results for: MAK;
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TypeBlog PostPublishedSept. 29, 2015, 2:30 p.m....pSiM24, can also be used with biolistic methods, making it useful for engineering plants that are not susceptible...available pCAMBIA and pKYLX vectors, respectively, making it ideal for large gene transfer. The M24 promoter...
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Mapping the 4D nucleome with CRISPR/Cas9
TypeBlog PostPublishedAug. 11, 2015, 2:30 p.m....Cas9’s ease of use and ability to label live cells make this system truly advantageous. This new technique...altered in disease states. dCas9 gets a colorful makeover Even without its nuclease activity, catalytically... -
6 Steps to Submitting a Resume That Gets Seen
TypeBlog PostPublishedJuly 9, 2015, 2:30 p.m....: if there is no place to upload a cover letter, make your cover letter the first page of your resume ... don't ask, you will never get the help that can make a difference. Having your resume forwarded personally... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog PostPublishedMay 12, 2015, 1:32 p.m....sought to make complex genome modifications in Drosophila using multiple recombinases. To make multiple... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog PostPublishedSept. 15, 2014, 6:05 p.m....there, but the panelists were interesting enough to make up for the somewhat naïve questions. During the ...coincidentally speak the same Polish sub-dialect making their Skype communications extra fun) who shared... -
London Calling - Addgene’s New Europe Office in London
TypeBlog PostPublishedJune 10, 2014, 3:20 p.m....interacting with people and talking about science makes her the perfect Addgenie and she can’t wait to see...even visit your lab! We look forward to helping you make some serious discoveries. 10 Years of Plasmid... -
CASTing Off for New Shores in Human Genome Editing
TypeBlog PostPublishedJune 10, 2025, 1:15 p.m.....1038/s41586-019-1323-z Peters, J. E., Makarova, K. S., Shmakov, S., & Koonin, E. V. (2017). Recruitment... -
Antibodies 101: Affinity Tags
TypeBlog PostPublishedSept. 19, 2023, 1 p.m.... a little thoughtful planning, affinity tags can make protein purification a cinch. Types of affinity ...that is unlikely to perturb protein function. This makes it amenable to being left on your protein of interest... -
Five Questions to Ask If You're Deciding Between a Master's or PhD Program
TypeBlog PostPublishedFeb. 16, 2021, 2:15 p.m....a means to gain employment, so it’s important to make sure a masters helps you get a job you want. But...bench. So if you enjoy wielding a pipette, it might make sense to earn a master’s instead of a PhD. Deciding... -
The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place
TypeBlog PostPublishedJan. 21, 2020, 2:30 p.m....in large supplementary data files, BioGRID ORCS makes it possible for researchers to query all public ...conditions. This increased ease of access not only makes CRISPR screen results available to the entire biomedical... -
Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight
TypeBlog PostPublishedJuly 16, 2019, 12:59 p.m.... with short generation times and high fecundity, make zebrafish ideal for high throughput studies of optogenetics...depends on these charged particles and whether they make the inside of the neuron more positive or more negative... -
Running for Rare Disease, Running for FOP, Running for AJ
TypeBlog PostPublishedSept. 15, 2016, 2:30 p.m....in healthy patients. However, the R206H mutation makes ACVR1 responsive to members of the ubiquitous activin...awareness of FOP. Please consider running with us or making a donation via the link below. Please continue ... -
Management for Scientists: Managing vs. Leading
TypeBlog PostPublishedMarch 31, 2015, 4:08 p.m....in doer mode even late in our careers. Managers make sure the work gets done Managers do this by removing...communication. A manager must both hire and fire to make sure the team is the best it can be. Managers must... -
Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio
TypeBlog PostPublishedJan. 15, 2015, 1:50 p.m.... my way to the meeting, and its history of code making and code breaking is wonderfully analogous to genetic...thanks to all the Addgenies who worked so hard to make this poster, offered suggestions and tips, and helped... -
10 Ways to Share Your Science!
TypeBlog PostPublishedMay 13, 2014, 8:34 p.m.... to share your science 1) If you don’t already make it a habit to talk – really talk – about the research...science, they have good advice for anyone looking to make their science matter. If you aren’t ready for a ... -
Plasmids 101: Antibiotic Resistance Genes
TypeBlog PostPublishedJan. 30, 2014, 3:29 p.m....scientists rarely stop to think about how much easier it makes our lives. Plasmid transformation into E. coli is... strains have natural antibiotic resistances, so make sure your plasmid and E. coli strain are compatible... -
Choosing a Good Mentor for Scientists
TypeBlog PostPublishedJan. 16, 2014, 2:56 p.m....serve as a good mentor. To read more about what makes a good mentor, see the previous post in this series...posts in our Science Mentors series: Part 1: "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring... -
Intro to Our Summer SciComm Series!
TypeBlog PostPublishedJune 9, 2022, 1:15 p.m....science communication research. This summer, we’re making time to talk about my favorite thing - science ...but the foundation of all good communication is making sure everyone’s on the same page. So for our first... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog PostPublishedJune 2, 2020, 1:15 p.m....summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...has developed a new CRISPR screening method that makes use of guide RNAs with internal barcodes (iBARs)... -
Plasmids 101: TOPO Cloning
TypeBlog PostPublishedOct. 27, 2016, 2:30 p.m....extra extension time after the last cycle of PCR to make sure that the "A" gets added to all PCR products... the standard ligation reaction is fairly quick, make sure you stay organized and prepare everything you...