We narrowed to 794 results for: Mpl

...experimental purposes. But what now? MoClo really simplifies vector construction by combining restriction ...process to produce a multigene construct. Figure exemplifies the extraction and use of a coding sequence of...four-base overhangs flanking each genetic part. (A) Example Level 0 assembly. BpiI recognition sites and four-base... Level 0 acceptor (bottom) for CDS1 parts. (B) Example Level 1 assembly. The choice between different ... MoClo user, but beginners or those performing complex assemblies should screen a minimum of 8–10 colonies...

...were most efficient at complex edits, such as long or highly structured templates. Cas9 variants PE6e–g...PE6b was very efficient for short, unstructured templates and significantly reduced undesired edits (indels...also make CAR-T cell therapy more accessible by simplifying the production process and could even enable ...tet-responsive element (TRE) or the cFos promoter. For example, neurons treated with a phorbol ester activator...

...The types of projects mentioned above are just examples, and healthcare consulting firms can be involved...investments over the next 3, 5, or 10 years. Complicating these challenges is the fact that they occur...their jobs most, get pleasure from taking up such complex challenges, quickly breaking them up into smaller... the unpredictability of schedules can take on employee morale and productivity, and many have mechanisms...that, most consulting firms coach and counsel employees on the different personality and work styles (...

...Lentiviruses are a powerful laboratory tool often employed to establish cell lines that stably express a ... for using lentivirus, infect and select, seems simple, in actuality, many find using lentivirus to be...al. outline a do it yourself PCR-based approach complete with freely available positive controls. Routinely...buffers used, and other experimental parameters. For example, while freeze thaw is generally not recommended...circumvent this problem, transductions typically employ the positively charged polycation, polybrene. Interestingly...

...manipulated for use in molecular biology studies. For example the E. coli biotin ligase, BirA, has been used ...corresponds with human diseases and conditions. For example, the rat is frequently used to study human cardiovascular...abilities. The axolotl has the ability to regenerate complex structures such as limbs, the spinal cord, and ...them an ideal model organism; they are relatively simple to maintain and breed and they have large, easily... and microinjections. Injections of mRNAs, for example, can be analyzed to understand human disease alleles...

...Brainbow multicolor labeling system - a perfect example of the continued utility of Cre-lox. Check out ...recombinations cannot occur. While the Brainbow-1 system employs Cre-mediated deletion, the design of Brainbow-2.1...Brainbow-2 each contained a “default” state; for example, Brainbow-1.0 expresses RFP when the construct ...system, Sanes and Lichtman also developed a complementary Flpbow construct that is functionally similar...technique will provide important insights into the complicated physical organization of the brain and other ...

...consider when choosing one.     Figure 1: Example of an experimental optogenetic setup. A light source...awry in a disease state.      Figure 4: Example of an intracellular signaling pathway activated...succession and maintain fidelity with the stimulus. For example, if you want to stimulate at a fast frequency without...stimulation. GPCR signaling GPCR signaling is a complicated process involving many downstream signaling mechanisms... pathways. Additionally, as GPCR signaling is amplified in successive steps along a pathway, there can...

...). Mycoplasmas are considered to be one of the simplest and smallest bacteria. The absence of a rigid ... of powdered media. Filtration does not ensure complete sterilization as mycoplasma can even escape 0.2... growth of mycoplasma but cannot eradicate it completely. When mycoplasmas are seen in the culture, it... changed for at least two or three days before sampling (Uphoff and Drexler, 2011). Culturing on agar... of mycoplasma. These methods cannot guarantee complete removal of mycoplasma but, can get rid of most...

...the planet, in which case whoa! Mission accomplished! One example of a mission-based team in academia would... have a specific purpose and finite goals. For example, when it came time to update how we make and distribute...transfer expert who understands the somewhat complicated Material Transfer Agreement process that kits...function. Project team A Project Team is assembled to complete a series of tasks with precisely defined goals...

...dsDNA was short enough, a ssDNA whose entire complementary strand was degraded. Beta: Beta protects the...created by Exo and promotes its annealing to a complementary ssDNA target in the cell.  Only Beta expression... inserts can be made by PCR using primers that amplify the DNA sequence of interest and flank it with ...region of interest and design an oligo that is complementary to the lagging strand. In practice, it’s easier...system once recombinant clones are generated.  A simple way to do this is to express lambda red genes from...

...gene/target, name, application, pass/fail rating, sample species, antibody species, or whether the results... by Addgene's curation team for accuracy and completeness. The information is then formatted and published...application. The submission process is easy, with simple, descriptive fields, drop-down boxes when appropriate...can work through it at your own pace. To submit, simply click "Contribute Data."    Figure 6: Contributing...

...a world of possibilities in the engineering of complex gene networks. A comparable system for targeting... of RNA is highly desirable for extending the complexity of genetic circuits, allowing for tighter spatio-temporal... of interest. The new tool was developed and implemented by applying the Golden Gate cloning method to...in addition to their use in basic biology, for example, by targeting pathways underlying cancer or other...

...Binghamton University Cancer is one of the greatest examples of survival of the fittest. Cancer cells find ...immune response to the development of tumors is a complicated and dynamic phenomenon that is interestingly ... also known to precede cancer development, for example, inflammatory bowel disease is associated with ...the expression of the major histocompatibility complex (MHC) class I proteins on their surfaces to avoid...and suppression of tumor-aiding cytokines, for example, will more broadly activate the immune response...

...cytometers. These fluidic machines handle biological samples and are cleaned between each use. You wouldn’t ...high-throughput and efficient way? Grenova, for example, offers automated, benchtop, high throughput washing...lab culture, but with the right incentives (for example, by grant agencies), we can shift toward a more...more sustainable one. The math on this is pretty simple: wash your tips once and you cut your tips cost ...

...recombination by KD, B2 and B3 resulted in complete or near-complete excision of the stop sequence. In contrast...toxicity, but with the caveat of partial rather than complete recombination. If you’re looking to use site-specific...this system. Gerald Rubin’s lab sought to make complex genome modifications in Drosophila using multiple...

...Scientists are excited by somewhat unusual things. For example, I am not that interested in meeting movie stars...this over 30 minutes, which was fun to watch. A simpler 1 protein - 1 RNA system Drs. Dounda and Charpentier...into one RNA to direct cleavage the result was a simpler 1 protein - 1 RNA system.  Dr. Charpentier advocated...breakthrough. In fact, the panelists agreed that, for example, the technology is already available today to cure...

...gRNA are not to scale.   Let’s start with the simplest multiplexing situation: you only need to express... and two U6 promoters. To use this plasmid, you simply order oligonucleotides for your chosen gRNA target...number of gRNAs you wish to clone, from 2-7. For example, if you’re expressing 4 gRNAs, you’d use pX330A...using BsaI, and promoter-gRNA fragments are PCR amplified for cloning into one of three Zeamays codon-optimized...especially useful for combinatorial analyses. For example, if you were to develop an array using 3 distinct...

...natural transdifferentiation is rare in mammals, an example is observed in the pancreas when excess β-cell ...1987, Davis et al. reported one of the earliest examples of transdifferentiation in vitro where treatment...vitro Both in vitro and in vivo Epigenetic marks Complete removal to convert to iPSCs and re-establishment...in science communication and publishing after completing her postdoctoral training from Massachusetts ...human induced pluripotent stem cells from urine samples. Nat Protoc, 2012. 7(12): p. 2080-9. PubMed PMID...

... had to get used to the workspaces, as well as complete the lab safety and cybersecurity training before...project is a major project given to each intern to complete and present on.    Figure 1: Addgene's...obtain eight clones. We ran digested and undigested samples of the clones on an electrophoresis gel in order...Toj-Villagran is a rising senior in high school who just completed their summer internship at Addgene. They plan ...

...Suitability for in vivo experiments, due to their simple structure    sdAbs in research These advantages...becoming valuable tools for diverse applications. For example, RANbodies can be used as an alternative to conventional...manipulate cellular functions, like in this early example we highlighted back in 2015, where sdAbs were used...around using sdAbs in diagnostic applications. For example, fluorescently and radioactively labeled sdAbs ...