We narrowed to 628 results for: Ncl
-
TypeBlog PostPublishedOct. 9, 2018, 12:21 p.m....stop and plan it out. Some questions to answer include: What is the goal of the experiment? What are...Howard Hughes Medical Institute. His interests include the quality control of secretory proteins in the...
-
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog PostPublishedOct. 17, 2019, 12:28 p.m....Microbial rhodopsins are encoded by opsin genes, and include bacteriorhodopsins, halorhodopsins, and channelrhodopsins...). Not so long ago, however, it was completely unclear which subpopulation of DA neurons could activate... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog PostPublishedDec. 17, 2020, 1:30 p.m....Cre recombinase contained within the knock-in. Including Cre-mediated marker excision, it takes approximately...that recipients for the 2015 Breakthrough Prize included Jennifer Doudna and Emmanuelle Charpentier for... -
Live and Let Dye: Self-Labeling Protein Tags
TypeBlog PostPublishedApril 16, 2024, 1:15 p.m....with minimal cross-reactivity. Other strategies include using engineered ligases to attach a ligand to ...choosing a dye or ligand to use for your experiment. Conclusion Researchers and vendors continue to develop new... -
Deep Dive: Statistical Tests (Comparisons)
TypeBlog PostPublishedMarch 7, 2023, 2:15 p.m....this time, it is! Factorial ANOVAs include two-way ANOVAs but can include many more than two independent ... -
Plasmids 101: Using Transposons in the Lab
TypeBlog PostPublishedJuly 6, 2021, 1:28 p.m....in yeast, plant, insect, and mammalian cells, including human cells. piggyBac is biased to integrate at...all been used to generate transgenic animals, including zebrafish, mice, rats, and rabbits (Sandoval-Villegas... -
A Guide to Getting Started in Undergrad Research
TypeBlog PostPublishedJune 8, 2021, 1:15 p.m.... still valuable for anyone in the lab though, including undergrads. I found a lot of career resources ...in microbes until the end of my second year! Conclusion and other pieces of advice Give yourself permission... -
Typing CRISPR Systems
TypeBlog PostPublishedMarch 18, 2025, 1:15 p.m....CRISPR will likely depend on a variety of factors, including your goal, experience, hypothesis, and even the..., D., Shah, S. A., Siksnys, V., Terns, M. P., Venclovas, Č., White, M. F., Yakunin, A. F., . . . Koonin... -
How to Deposit Your Plasmids with Addgene
TypeBlog PostPublishedFeb. 27, 2024, 2:15 p.m....information related to your plasmid. It is optional to include the total size of the insert and the GenBank ID...submission, please email us at [email protected]. Include your deposit number and which data needs to be ... -
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs
TypeBlog PostPublishedNov. 3, 2015, 3:30 p.m....software for use on any machine with OpenCL enabled hardware. This OpenCL dependency is a severe limitation... -
Delivery Methods for Generating iPSCs
TypeBlog PostPublishedApril 17, 2018, 1:37 p.m.... Paramyxoviridae family of viruses, which also includes measles and mumps. Sendai transduces a wide range...the field of iPSCs Find gene-specific plasmids (including reporters and RNAi) for: NANOG OCT4 SOX2 MYC... -
Addgene Launches Partnership with IPI
TypeBlog PostPublishedJune 13, 2023, 1:15 p.m....educational resources on our website and blog, including our antibody guide, antibody protocols, and Antibodies... -
Thanks to Addgene Depositors, We’re Now Sharing over 100,000 Reagents!
TypeBlog PostPublishedApril 8, 2021, 7:25 p.m....globe, we’re now sharing over 100,000 reagents including plasmids, ready-to-use viral preps, pooled libraries... -
Viral Vectors 101 eBook 2nd Edition
TypeBlog PostPublishedJuly 11, 2024, 1:15 p.m....AAVs in your experiment. Our second edition includes new and updated content in a clean and easy-to-navigate... -
CRISPR Guide Update
TypeBlog PostPublishedOct. 10, 2024, 1:15 p.m....content. Access the CRISPR Guide here! Highlights include a much-anticipated prime editing section and additions... -
Suggest A Plasmid You'd Like to Find at Addgene
TypeBlog PostPublishedMarch 16, 2016, 5:30 p.m....the specific plasmids you’d like to suggest and include any additional details that will help us reach ... -
Addgene's 20th Anniversary Party
TypeBlog PostPublishedJune 11, 2024, 1:15 p.m....some of the moments we captured over the years, including an album of Addgenie pet photos. The famous Addgene... -
Congrats to the 2019 Deck The Lab Winners
TypeBlog PostPublishedJan. 17, 2020, 3 p.m....from lab materials. This year, one of the winners included a lab material tree made from lab gloves. The ... -
New Videos: Addgene Lab Tips
TypeBlog PostPublishedFeb. 15, 2018, 2:41 p.m.... lab has gotten a lot experience in processes including automated vial filling, accurate tube-labeling... -
Twenty Years of Sharing Science!
TypeBlog PostPublishedJan. 8, 2024, 3:25 p.m....ourselves as a repository for DNA-based materials, including our popular kits and libraries. This DNA-based...