We narrowed to 860 results for: PLE

...   Fig. 1: A sample containing a polyhistidine tagged protein of interest (purple) and several contaminating...strategy a single protein can be isolated from a complex mix using affinity chromatography against an amino...metal ions are immobilized on a bead or resin and a sample containing the protein of interest flowed through...Ni2+-containing resin. The protein of interest (purple) binds to the Ni2+ and is immobilized along with...tags are also recommended if you need a very pure sample. Because binding strength is proportional to the...

... tools that expand what we can achieve, but are simple to pick up and work well the first time. But biology...something surprisingly useful. To apply these principles in a biological setting, we first need to define...Click Biology. Created with BioRender.com.   Principles of Click Biology Physical scientists have been...reactive groups of Click Chemistry are almost completely absent from living systems. What are the possibilities... kind of cellular computation, making possible multiple logic-gated steps. Beyond the lab, these systems...these tools are already deposited at Addgene in multiple formats: for use in different organisms, targeted...

...fluorescent proteins can be directly compared. An example of a photostability measurement of various cyan...results in a loss of fluorescence, which, in its simplest form, can be described by a mono-exponential decay...intensity decay of a fluorophore does not follow a simple mono-exponential decay (Shaner et al, 2008; Bindels...applications in which far less power is used. For example you might find that you can get useful data from... diffusion, the proteins are (i) trapped in microdroplets in a water/oil emulsion, (ii) embedded in a ...fluorescent protein. In figure 1 we provide an example of such a comparison between cyan fluorescent proteins...

...toxins are the clearest example of a plasmid benefiting its host. For example, cells with a penicillin...Shareck et al., 2004). Plasmid categorization is complex Plasmid classification has shifted over the years...Plasmids may also have more than one function: for example, Gateway cloning relies on genes found on an E....plasmids without clear analogues in nature. For example, helper plasmids were developed to replace helper... Multiple Authors...

... parents. Here’s how to cross mice, using a simple example with the goal is to generate mice that have...genome, they are not commonly used to introduce multiple mutations. This is because as more mutations are...challenge that may arise when crossing mice with multiple mutations is if the mutations are on the same ...expressing strains Cre-lox strains are a common example of mice that are derived from crosses. In Part ...With this two-part guide to mouse modeling now complete, you should be prepared to plan an experimental...

... features of the gene intact. Here, we show an example gene targeted for tagging with GFP at the C terminus...interest. For a C-terminal tag, as shown in the example above, we inserted a linker and GFP tag at the ...the GFP tag precisely where we need it, in this example, at the C-terminus of the gene of interest. To ...CRISPR/Cas9 (Cas9 protein and the crRNA-tracrRNA complex) and the donor plasmid into the cells we use electroporation... Institute for Cell Science Plasmid Collection, please visit the Cell Catalog on the Allen Cell Explorer...hiPS cell lines generated using these plasmids, please visit the Cell Catalog on the Allen Cell Explorer...

...public, for example at an anniversary or for Girls’ Day, offer guided tours to show people where the lab...into science education Do you aim to reach young people? Try networking with your local education community...Trust blog or the John Innes Student Voice are two examples. Many blogs like Plant Science today, Soapbox ...meetings. 8) Training – Get a formal education Some people get into science communication by taking a media...them to be the “be-all-and-end-all”. You can, for example, get an MSc Science Communication or MSc Science...

...buried beneath someone's lunch. Many times, I’m pleasantly surprised to discover that the most crucial information...from you and it’s wrong. Can you help us fix it? Please address as soon as possible!” and I always begin...your concern is based on data you have produced, please share the data. Maybe not all, but many bio companies...helping us understand the nature of your concern. Please be courteous. We understand how exasperating it...time and money on a potentially faulty product. Please remember that the person at the other end of that..., let your customer service agent know (nicely, please!). We can’t improve without feedback. And if you...

...Having never been to China myself, I was pleased to be able to participate when the organizers of the...Lowe lab have allowed them to begin understanding complex regulation of and by tRNAs and tRNA-derived RNAs...long hypothesized that life started with RNA and a simple cell membrane. His recent work shows that they ...replication with no proteins). The chemistry is complex and this work requires an interdisciplinary team... they do it. Of course, No conference would be complete without CRISPR Michael Terns from of the University...structural and functional component of the ribosome complex and Dr. Steitz quipped that RNA in the ribosome...

...to co-localize multiple enzymes in a reaction pathway, the MPyV system provides a simple, efficient method...SARS-CoV-2 by mutational scanning By Seth Kasowitz As multiple antibody-based vaccines are deployed around the...many aaRSs exist within a multi-tRNA synthetase complex, the team investigated whether the generated antibodies...antibodies were able to immunoprecipitate the entire complex in three different cell lines. The Gräslund lab...jbc.RA120.01289     Novel protein cage provides simple enzyme protection in yeast bioproduction pathways...

... passage number. Especially in a lab where multiple people are working on different things, this will ...in use. Wipe the hood with a disinfectant (most people use 70% ethanol, but you can also use Incidin wipes... this purpose.Learn about the effects of CO2 on sample pH Cell morphology: Shape and size matter You should...which multiplies every 16 hours). So if you, for example, split cells on a Monday diluting them 1:10, you...contaminated.Learn how Addgene uses barcodes to track samples Keep track of passage numbers Keep an eye on the...

...name for some context and maybe common ground. The people you meet may all be scientists but not all will...to practice beforehand. Generally, two types of people will visit your poster: those that want you to ...Try things like sitting at a table for lunch with people you don’t know or attending social events. I like...like to set goals for myself like meet ten new people each day. Exchange business cards. When you’re done...Follow up with those that you’ve met. Connect with people on LinkedIn or Twitter, for instance. If you’ve... head over to the “How to Make Friends and Meet People at a Scientific Conference” blog post. There’s ...

...through homologous recombination. The concept is simple: deliver a template with a modified version of ...homology is typical for targeting constructs. In the example shown in Figure 1, large sequences corresponding...is expressed (knocked in). As seen in the above example, you can remove a floxed resistance gene using ...plasmids described in the preceding sections are simple ways of knocking out a non-essential gene of interest...there are 3 loxP sites, recombination can occur in multiple ways. The desired recombination event will remove...tools for creating knockout animals or cell lines, please consider sharing them by depositing with Addgene...

...strengthen your results. Enrichment or depletion of multiple gRNAs targeting the same gene can be strong evidence...jar of jellybeans, a pooled CRISPR library is a complex mixture. Pooled libraries are composed of many ...sorting and screened for a particular phenotype. For example, mutant cells can be used in drug screens to identify.... Loss of representation due to enrichment or depletion of specific gRNAs can lead to skewed results. ...any experiment, the use of appropriate controls, multiple replicates and several cell types can strengthen...

...getting-started guide for BLAST, which includes a simple explanation of the different BLAST programs, databases...consistently run our blastx searches with the “Low complexity regions” filter unchecked so that these regions...benefit is that blastp searches do not filter low complexity regions by default, meaning that you do not have...Clustal is our frequent choice for pairwise or multiple sequence alignments of nucleotide or protein sequences... sequences. We also use COBALT for aligning multiple protein sequences, particularly for comparing different...//www.ebi.ac.uk/Tools/webservices/#multiple_sequence_alignment_msa http://www.ebi.ac.uk/Tools/webservices...

...allowing very low background level of fluorescence. Examples for such photoactivatable or photoconvertible ... and their emission is green or red light. For example, T-Sapphire, LSSmOrange, and LSSmKate. Fluorescent...sensor), and iGluSnFr (glutamate sensor). More examples of these biosensors can be found at Addgene. ...also is also called Bimolecular Fluorescence Complementation (BiFC). 8 points to keep in mind when choosing...system can excite, and detect the emission. For example, if your microscope has only two lasers, at 488nm...minutes after it is translated to a few hours. For example, superfolder GFP (sfGFP) and mNeonGFP can fold ...

... takes 1-1.5 hours to complete, while this syringe-based method can be completed in 20 to 30 minutes. ...drawbacks of kits and typically follow the same principles: cells are lysed, RNAs and proteins are removed... centrifugation. At this point, the plasmid DNA sample can be treated with RNase to remove RNA contamination...waste footprint. The Zhao and Deng labs developed a simple method for regenerating silica columns that come... reduce the number of spin columns you need to complete your research. Do you know of other nucleic acid...

...socks, persevered through a nerve wracking commute complete with a disabled train, and now you’re sitting ...within yourself, and to demonstrate them to the people who make hiring decisions. Though having all the...to other acquired skills.” It is nice when other people point out how awesome we are, but there is also...my wheelhouse”, take a second to reflect. For example, say your boss approaches your team with a project...front of a crowd and using your charisma to give people hope for some shiny vision on the horizon. You’...these responsibilities. If you are in charge of completing many meticulous protocols or tasks, you’ve probably...

... of genes and proteins in living plants, to complementing mutant phenotypes. Arabidopsis is amenable to... sweet potatoes (Kyndt et al., 2015)! It sounds simple, and it pretty much is, but there are some tips...There are a bunch of great options, but here are a couple of my favorites. Plasmids from the Tsuyoshi Nakagawa...you transform early, your plants will still have plenty of time to produce seeds after you expose the flowers...for transformants. It’s very important to select multiple T1 plants from each transformation. I like to ... Agrobacterium T-DNAs with expressed genes: An example of a naturally transgenic food crop.’, Proceedings...

...including depositing labs and institutions. For example, search for “Melina Fan” and you’ll be able to ...plasmids from a paper have been deposited? In this example we’re going to be looking for recombinant monoclonal...enough... If in the past you have tried to combine multiple terms in an Addgene plasmid search and found the...each filter is displayed in parentheses.  In this example I wanted to find a lentiviral plasmid to express...Starting with two search terms we see there are plenty of results, but I can’t browse through 300+ plasmids... plasmids that may be difficult to define for a simple search. These pages provide helpful information...