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We narrowed to 837 results for: RING

Showing: 361 - 380 of 837 results

  1. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type
    Blog Post
    Published
    May 25, 2021, 2:29 p.m.
    ...molecular definition, and potential for further engineering as compared to polyclonal or monoclonal antibodies... in this blog post. Hello, NABOR! Addgene will bring the same open-access benefits to antibodies that...collection. I’m thrilled to be working with Addgene to bring this resource to life,” says Dr. Trimmer. But in...allow Addgene to further our mission of scientific sharing,” says Dr. Daniela Bourges, Addgene’s interim Executive...

  2. Antibodies 101: Epitope Availability

    Type
    Blog Post
    Published
    Jan. 11, 2022, 2 p.m.
    ...researcher and ones that happen naturally.  Sample Denaturing  First, let’s think about induced conformation...s configuration in your assay. Did you have a denaturing step? Do you want to capture the protein bound...conditions? It can help to visualize an abstract protein during your sample prep process, imagining how it starts...antibodies that have been validated solely for denaturing assays. Many antibodies will note if they’re ...

  3. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...It’s nearing the end of #DepositorWeek at Addgene and we’d like to show our gratitude on the blog by ...ordered biochemical events” such as those that occur during development. Read this post to learn more about...research, check out this post! R Bodies: Membrane-Rupturing Microscopic Tools In this post, Jessica Polka,...consider if you’re getting started with them. CRISPR Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening...

  4. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ... each day brings in new and surprisingly diverse manuscripts. I work with people, nurturing networks, ...regulation of DNA replication and gene transcription during vertebrate embryogenesis. My interests, though,...other media outlets (AAAS Mass Media Science & Engineering Fellows Program). Science journalism programs...

  5. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...academic positions. More recently, I have been hearing exit survey data from postdoctoral programs in ... (every 4-6 years is common), but this can also bring new opportunities—projects end and layoffs occur...change entire culture) Specific requirements for hiring more common Likelier to hire on general talent... paths is an important first step. Developing mentoring relationships, especially with peer mentors, to...

  6. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...remarkable nine papers describing CRISPR/Cas9 genome engineering methods for C. elegans were released, signaling...revolution!   References Streamlined Genome Engineering with a Self-Excising Drug Selection Cassette....strategies for CRISPR/Cas9 editing in C. elegans: Engineering the Caenorhabditis elegans genome using Cas9-... Mary Gearing...

  7. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ...Griesbeck is motivated by a particular interest in monitoring the activity and biochemistry of living neurons...allowed the researchers to see tonic action potential firing in neurons and high resolution functional tracking...while in principle adapting a FRET sensor for monitoring calcium versus zinc should require the simple...cells at much lower concentrations than calcium, requiring greater sensitivity. He and his colleagues came...

  8. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...innovations have improved the odds of mismatch repair favoring the edited strand. The first iteration of prime...introduced, relies on the PE2 enzyme. PE4 and PE5: Engineering mismatch repair Even in PE3 and PE3b, prime editing...of the unedited strand Favor the edited strand during mismatch repair   ✓   ✓ ~ ~ ~ ...(2023). Phage-assisted evolution and protein engineering yield compact, efficient prime editors. Cell,...recombinases and prime editing. Nature Biomedical Engineering. https://doi.org/10.1038/s41551-024-01227-1 Yan...

  9. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...sequenced for some time, clearing the greatest hurdle for genome engineering. A unique evolutionary feature... traits. The “biotech yeast” You’re probably wondering if the “biotech yeast” is S. pombe or S. cerevisiae...small, membrane-bound organelles essential for sequestering certain enzymes and performing specific oxidative...

  10. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ... review, and more lab research could be used to bring scientists and the public together to build consensus... it is followed. He’s PCSK9 paper, though not featuring a clinical pregnancy, was rejected by at least...Download the CRISPR 101 eBook Read our genome engineering blog posts Resources on Addgene.org Read our...CRISPR plasmids by function Check out our genome engineering guide ...

  11. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...some of Addgene’s most exciting and versatile offerings! We recently re-amplified our distribution stock...sequencing. Maintain representation of the library during transformation First, use enough DNA and competent... a file in FASTQ format, a standard format for storing NGS reads and their corresponding sequencing quality...making sure few/no plasmids have dropped out, and comparing pre- and post-amplification sequencing results...

  12. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ...organization, mentoring group, or department. Even if it doesn’t work out, it’s flattering to ask.  Show...Relationships need nurturing. Perhaps you’ve met someone interesting at an event. Or, perhaps you have...There is a person on LinkedIn I once helped by answering one simple question so I accepted the connection...

  13. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...Old Dominion University, who is majoring in marine biology and minoring in studio arts photography.  References...specific procedural instructions, including timing, preparing the smear, and mixing the stains and solutions.... & Walker S. The Bacterial Cell Envelope. Cold Spring Harb Perspect Biol. 2(5) a000414 (2010). Cleveland...

  14. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...limitations: they were not optimal for experiments requiring high signal to noise ratios, imaging of high frequency...Create yellow (v82) and lime green (v857) variants differing in kinetics, affinity, and ∆F/F0. Introduce the...and localization through different C-terminal anchoring domains The original iGluSnFR in the pMinDisplay...researchers introduce iGluSnFR3 in two additional anchoring domains – GPI and SGZ. Fig: 4 Sequence...

  15. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...S.H., Varmus H.E. Retroviruses. Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 1997. Principles...ITRs are perfectly symmetrical to allow for this pairing to occur. Like LTRs, the ITRs define the limit ... supplied by the production cell line (HEK-293) during production. Fun fact: There are 57 human adenovirus...

  16. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...expanded for multiplexed imaging for advanced monitoring of different factors, in different or the same...stimuli and as well as changes in cellular dynamics during so-called housekeeping activities.     ...cell (sub)types for expression and downstream monitoring. Nervous about making a virus for your biosensor...Biosensors for Neurotransmission and Neuromodulation: Engineering and Applications. Frontiers in Cellular Neuroscience...

  17. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    Published
    March 17, 2020, 1:32 p.m.
    ... adenine base editor Lower-stringency PANCE, followed by higher stringency PACE, resulted in the evolution...which results in less overall dilution and less stringent phage selection. Figure 2: Phage-assisted...base editors? Targeted evolution of naturally occurring enzymes and functional domains speed up the development...

  18. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...milk processing, fruit juice clarification, meat tendering, and oligosaccharide synthesis. A major incentive...insoluble granules. We overcame this problem by engineering a plasmid which contained cold tolerant chaperones...editing for psychrophilic systems as well as engineering and regulating psychrophilic recombinant proteins...synthetic biology experiment Read our Genome Engineering Guide ...

  19. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...for supporting scientific sharing. We’re very excited for more reagent sharing projects in the years to...15 years of plasmid sharing has certainly taken us on many adventures...From moving office locations ...Cas9 (SpCas9) has been widely popular in genome engineering. For use in human cell lines, the Zhang lab codon-optimized...

  20. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...immune response. There are three main pathogen clearing functions or effector functions of the constant...each of the isotypes. IgM This isotype is produced during a primary immune response because all B cells begin...found in tissues. IgG is the predominant isotype during a secondary immune response, which is when the ...constant domains. This switching helps B cells adapt during the course of an immune response since it also ...
Showing: 361 - 380 of 837 results