We narrowed to 488 results for: Ret;

1. Choosing the B(right)est Fluorescent Protein: Photostability

   Type

   Blog Post

   Published

   June 8, 2017, 2:30 p.m.

   ...Amsterdam) where she develops genetically encoded FRET based biosensors.     Additional Resources on the...

2. RANbodies: Reporter Nanobody Fusions

   Type

   Blog Post

   Published

   April 10, 2018, 12:56 p.m.

   ... RANbodies are expressed in 293T cells and are secreted from cells due to their IgGκ signal peptide sequence...

3. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

   Type

   Blog Post

   Published

   Feb. 2, 2018, 3:20 p.m.

   ...grad school with the plan of being a scientist. I pretty quickly figured out that I just don’t have the ...

4. Visualizing Protein Turnover In Situ

   Type

   Blog Post

   Published

   Jan. 16, 2018, 3:20 p.m.

   ...on Addgene.org Fluorescent protein plasmids Use FRET to study protein-protein interactions Deposit plasmids...

5. To Each HIS Own

   Type

   Blog Post

   Published

   March 2, 2023, 2:15 p.m.

   ... specific proteins within a system. This can be pretty complicated given that a single cell has tens of...

6. Antibodies 101: Polyclonal Antibodies

   Type

   Blog Post

   Published

   July 14, 2021, 1:15 p.m.

   ...antibodies in its bloodstream, the antibodies are retrieved first by drawing blood from the immunized animal...

7. Plasmid Tools for Microbiome Studies

   Type

   Blog Post

   Published

   June 27, 2019, 12:25 p.m.

   ...with broad host-range (Meyer, 2009). The vector retains the oriT for delivery into recipients by conjugation...

8. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

   Type

   Blog Post

   Published

   March 19, 2019, 1:31 p.m.

   ...study PPI using Förster Resonance Energy Transfer (FRET, Heckler et al., 2015) or ratiometric Bimolecular...

9. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

   Type

   Blog Post

   Published

   April 13, 2017, 2:30 p.m.

   ...synthesized at the ER, delivered to the vacuole via the secretory pathway, and then cleaved to generate an active...

10. CRISPRainbow and Genome Visualization

    Type

    Blog Post

    Published

    Feb. 28, 2017, 3:30 p.m.

    ...Cas9 (dCas9) cannot induce gene editing, but it retains its gRNA-directed targeting capacity. By using ...

11. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type

    Blog Post

    Published

    Dec. 8, 2016, 3:30 p.m.

    ...mutants coding for non-cytotoxic proteins which retain their regulatory functions.” Mol Microbiol. 1995...

12. Developing Lab Management Software for Biology

    Type

    Blog Post

    Published

    Aug. 25, 2016, 2:30 p.m.

    ...plasmid and now it’s time for our lab technicians to retrieve the sample and get it ready for shipping. In the...

13. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type

    Blog Post

    Published

    April 12, 2016, 2:30 p.m.

    ...storage vector to the pAWp12 lentiviral vector should retain the diversity in your gRNA library, but this should...

14. The Future of Research Symposium Boston 2015

    Type

    Blog Post

    Published

    Oct. 20, 2015, 2:30 p.m.

    ... Boston 2015 where we hope to begin developing concrete solutions to the problems looming over the academic...

15. Career Coaching for Scientists: Why and Where Do I Find One?

    Type

    Blog Post

    Published

    Jan. 29, 2015, 1:25 p.m.

    ...us) offers a free report on her website called “Secrets to Success in Graduate School”. She has also launched...

16. Working with Nuclear Receptors

    Type

    Blog Post

    Published

    July 15, 2014, 4:03 p.m.

    ...signaling to peripheral effector organs, which then return information such as nutritional status. Within ...

17. Hot Plasmids and Viral Preps - January 2021

    Type

    Blog Post

    Published

    Jan. 5, 2021, 2:15 p.m.

    ...serotypes from the Sanford Boye lab with enhanced retinal tropism. Find the upcoming serotypes here ...

18. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type

    Blog Post

    Published

    June 25, 2020, 1:15 p.m.

    ...wanted to get her PhD. “Going back to school was a pretty easy choice for me,” she said. “I had always been...

19. Engaging with science and society at pgEd

    Type

    Blog Post

    Published

    Sept. 20, 2018, 12:17 p.m.

    ...digital academic publishing for a year, I’ve since returned to pgEd as a full-time staff member for the past...

20. Adenoviral Vector Production and Troubleshooting

    Type

    Blog Post

    Published

    June 14, 2018, 11:44 a.m.

    ...AdV) are devoid of all viral coding sequences and retain only the ITRs and packaging signal in cis. Therefore...