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...how to amplify recombination-prone plasmids and testing your prep can save you the headache of troubleshooting...colony you selected contains the full plasmid. A diagnostic digest is one of the easiest and clearest ways...you can try re-streaking for single colonies and testing multiple preps. In some cases, we suggest gel extraction...plasmid is present, but the recombined plasmid is still visible. This indicates that the DNA prep contains...

...tips for scientists working at home, and you will still find this useful. Now that we’ve been doing this...just need to know you well enough to ask good questions and hold you accountable for your goals.  I’ve...interviews are 1:1 discussions with someone who has interesting experiences to share. The conversation builds...edit everything you’ve tried to draft, but you’ll still be practicing and getting familiar with the process...

... have the potential to provide improvements to existing technologies based on polymerase processivity....stable, highly soluble, and non-immunogenic yet are still just as specific to their target as regular antibodies...podcast segment Steven Carr’s lab at the Broad Institute focuses on proteomics: developing and applying...analysed. In this work, the authors specifically investigated protein binding at the TERT gene, but this method...

...University, and the Karolinska Institute developed mNeonGreen via 21 substitutions to LanYFP and the addition...Nathan Shaner and colleagues from The Scintillon Institute, Allele Biotechnology, Florida State University...applications from traditional imaging to FRET and stochastic single-molecule super resolution imaging. mNeonGreen...

...2016 Addgene’s research team has been actively investigating ways to improve viral vector production. Whether...Whether redesigning viral packaging plasmids, investigating different viral production strategies or developing...were unable to deduce the mechanism responsible we still believe that these results will be beneficial to...method to improve AAV yields. Although we only investigated 3 serotypes it’s possible that this approach...

... replicate the host's chromosomal DNA, but they still rely on the host machinery to make additional copies...Patrick says researchers can ask themselves a few questions to get started: Will the plasmid be used exclusively...that plasmids with low to medium copy numbers can still express massive amounts of protein given the proper...cells have not started to die off. Have any questions? Need something clarified? If so please, post ...

... mountain – with many routes up and only one destination – a career in the sciences is more like an intergalactic...developmental programs unfold, absorbing the stochasticity of molecular interactions and embedding individual... weird and rare organisms so I found it hard to stick to vertebrates alone.  And a longstanding inclination...with things like word choice, punctuation, and stylistic consistency. If you’ve ever doubted the importance...

... of the primer assist with restriction enzyme digestion (usually 3-6bp) Restriction Site: Your chosen...and then TAAGCA to improve restriction enzyme digestion. This gives us a sequence of 5'-TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA... You can generate the reverse-complement using existing software (a quick internet search will lead you... The PCR product is now ready for restriction digestion. As such, the later steps in this process are ...

...labs. We’re overjoyed to provide you with these fantastic educational resources and will be sure to keep...us at [email protected]. Thank you again for a fantastic year and happy reading! Top 10 blog posts of 2015...UNC Chapel Hill explain some of the molecular gymnastics behind DNA repair by non-homologous end joining...career coaches do and offers up some career coach suggestions. 8. A Match Made in Heaven: CRISPR/Cas9 and ...

...Serendipitous Stain. https://hardydiagnostics.com/wp-content/uploads/2016/05/Hans-Christian-Gram.pdf Smith, A. ...infected lung tissue, a Danish biologist named Christian Gram realized that certain stains were retained...Stain. https://my.clevelandclinic.org/health/diagnostics/22612-gram-stain Hardy, J. Gram’s Serendipitous...

...for sleep. At Addgene, our mindfulness group is sticking with their Headspace practice even while working...for bench scientists to assist in labs that are testing for COVID-19. You can also give back by supporting...interview with you, or look for professional societies hosting virtual networking events. Download Addgene’s Science...expectations for your productivity Set a routine and stick to it. Plan defined work hours as well as time for...

...complicated entities with a large range of characteristics (color, brightness, photostability, maturation...can submit new fluorescent proteins, add data to existing proteins when missing, or edit inaccuracies as...these parameters and you’ll find matching FP suggestions. If you’re not sure exactly what you’re looking...Plans for future development of FPbase FPbase is still very much in the early stages of development, and...

...and white. The decor was very contemporary and futuristic with block chairs in the hallway for people to...what to expect at a workshop and what kinds of questions to ask myself when I am bombarded with a lot of...’m hoping to learn from this workshop? These questions will help me avoid being overwhelmed by all the...instead allow me to pay attention and answer my key questions. As an added bonus, I also got the opportunity...

... T helper type 2 (Th2) cells from mice that constitutively express Cas9 and screen for genes that affect... Steven Farber and colleagues at the Carnegie Institute for Science have developed the LipoGlo system ...UAS). The Wang Lab created a tripartite system consisting of dCas9, a gRNA fused to an RNA binding motif...displays high dynamic range. However, the promoter in question must be σ54-dependent and the system may not be...

...scientific question that they are eager to discuss with others and sending out these questions ahead of ...Figure 1: Virtual lab meeting? No problem! You can still creatively collaborate and brainstorm! Image from... be written by several authors from different institutes, disciplines, and cultural backgrounds.” This...

...Relationships need nurturing. Perhaps you’ve met someone interesting at an event. Or, perhaps you have a colleague... “it was nice meeting you.” Include follow-up questions or requests for advice based on your discussion...LinkedIn I once helped by answering one simple question so I accepted the connection invitation. They ...became closer. She supported me and answered my questions and honestly, she was the only person who really...

...just focus on the parts where people had questions or suggestions, instead of reading the whole thing together...gets a chance to talk and less vocal team members still have their voices heard. Here's a template for team...other’s projects. For all-company meetings, monthly still works best. Figure out what works for you for each...

...biology – they can deliver materials to specific destinations, haul a variety of cargo, and even handle a ...cassettes typically contain a promoter (inducible or constitutive), your gene of interest, and often a selectable...your construct in target cells transiently or constitutively depending on the virus used. All of these ...response 2) very high immunogenicity 3) and long-lasting immune response even after a single dose. ...

...experiment.  For example, I used to constantly underestimate time needed to prep for an experiment (labeling...protocol, from a full page to a few key reminders on a sticky note   Visual Planning  Plate maps, pre-labeling...you need to be successful! Slowing Down At the suggestion of a postdoc, to whom I am forever grateful, ... of things, including (and maybe especially) suggestions you are initially resistant to. Just like any...

...answer to any of these questions is “yes,” then I would suggest either testing the antibody efficacy through...incident to your records, and consider the following questions as you move forward:  Was a light-sensitive conjugate...for your ELISA and Western, I’d highly recommend sticking with what works.  To balance this out, however...