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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 610 results for: STO;

Showing: 361 - 380 of 610 results

  2. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ...to late-career grant professionals have similar stories about a time when their passion for an organization...their new career path.  A part of the career path story for most established grant professionals is learning...

  3. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...including MTA and shipping. Addgene realizes the One-Stop shopping for a wide range of plasmids. The merit... Their progress might be slow, but if they don't stop, they may be able to achieve something that may ...

  5. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...unmarked genetic modifications in the yeast Pichia pastoris. By combining both positive and counter selection...marker for unmarked genetic modification of Pichia pastoris." FEMS yeast research 9.4 (2009): 600-609. PubMed...

  6. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...64 possible codons but only 20 amino acids and 3 stop codons to encode leaving 41 codons unaccounted for...preferences among organisms aren’t completely understood, but some possible reasons include: Metabolic...

  7. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...produced in. For example, production of vesicular stomatitis virus, HIV-2, and human foamy virus in cell lines...protein for pseudotyping is VSV-G, or vesicular stomatitis virus G protein. VSV-G is a trimeric protein ...

  8. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...pTnsABC, pQCascade and pCutamp.  pDonor carries a customizable cargo gene in the form of RE-cargo-LE (RE, right...and the crRNA. The spacers of the crRNA can be customized as well, including concatenations into crRNA ...

  9. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...career solving hard problems or who needs a stepping-stone to many other opportunities. Many thanks for our...Bioengineering and works as a healthcare consultant in Boston. He is interested in the business of life sciences...

  10. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...of such targets include transcription factors, histones, and DNA repair enzymes. Note that when using ...stepwise decrease in fluorescence intensity in a (histogram) flow plot (Figure 4b). Through this method, differences...

  12. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...cloned by two separate labs (Bird et al., 1988; Huston et al., 1988). scFvs are the smallest unit of an.... https://doi.org/10.1371/journal.pone.0189964  Huston JS, Levinson D, Mudgett-Hunter M, Tai MS, Novotny...

  13. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...edition. Cambridge university press, 1988. AAS: Historical Trends in Federal R&D NJ.com: Rush Holt: Lawmakers...affected by Trump’s immigration ban Studies in History and Philosophy of Science Part: Democratic values...

  14. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ... al is a PCR-based approach for generating a customizable comprehensive mutagenesis library that’s ready...sites in the same orientation as the gene or use custom gene synthesis or site directed mutagenesis to ...

  15. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...respectively. H, A, and W describe the shape of a histogram, which graphically represents the time and intensity...graph of photocurrent vs. time. The resulting histograms is described through maximum current (H), the...

  16. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...However, ART is not an HIV-1 cure. In patients who stop ART, viral levels will soon skyrocket back to pre-treatment...HIV-infected reservoir, researchers have previously used histone deacetylase (HDAC) inhibitors to increase transcription...

  17. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ... to the new genomic locus. Image courtesy of Christopher Fell, PhD. After minimizing the system's...enhancer-AAV constructs are now available as in-stock viral preps using the systemic PHP.eB capsid, to...

  18. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 2:15 p.m.
    ...Availability of AAV serotypes at Addgene. Serotype In-stock viral prep Packaged on Request AAV1  ✓ ✓ AAV2... Data Hub AAV Packaged On Request Browse all in-stock AAV preps Browse all AAV plasmids ...

  19. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...ICC) when the sample is cultured cells or immunohistochemistry (IHC) with tissues), where an antibody binds....3001161 McLaughlin, K. (2019). Multiplexing Immunohistochemistry. Materials and Methods, 9. https://doi.org...

  20. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...pUC multimerization was reduced by growing seed stocks at 30°C compared to 42°C. Screen multiple colonies...platform incorporating low metabolic burden seed-stock and fed-batch fermentation processes. Biotechnol...
Showing: 361 - 380 of 610 results