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We narrowed to 611 results for: STO;

Showing: 361 - 380 of 611 results
  1. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ..., M., Castro, D., Karr, M., Hawkins, L. K., & Hermiston, T. W. (2003). Multigene expression from a replicating...PMID: 17510320. Tollefson, A. E., Scaria, A., Hermiston, T. W., Ryerse, J. S., Wold, L. J., & Wold, W....
  2. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ...to late-career grant professionals have similar stories about a time when their passion for an organization...their new career path.  A part of the career path story for most established grant professionals is learning...
  3. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...including MTA and shipping. Addgene realizes the One-Stop shopping for a wide range of plasmids. The merit... Their progress might be slow, but if they don't stop, they may be able to achieve something that may ...
  4. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...unmarked genetic modifications in the yeast Pichia pastoris. By combining both positive and counter selection...marker for unmarked genetic modification of Pichia pastoris." FEMS yeast research 9.4 (2009): 600-609. PubMed...
  5. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...64 possible codons but only 20 amino acids and 3 stop codons to encode leaving 41 codons unaccounted for...preferences among organisms aren’t completely understood, but some possible reasons include: Metabolic...
  6. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...produced in. For example, production of vesicular stomatitis virus, HIV-2, and human foamy virus in cell lines...protein for pseudotyping is VSV-G, or vesicular stomatitis virus G protein. VSV-G is a trimeric protein ...
  7. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...pTnsABC, pQCascade and pCutamp.  pDonor carries a customizable cargo gene in the form of RE-cargo-LE (RE, right...and the crRNA. The spacers of the crRNA can be customized as well, including concatenations into crRNA ...
  8. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...career solving hard problems or who needs a stepping-stone to many other opportunities. Many thanks for our...Bioengineering and works as a healthcare consultant in Boston. He is interested in the business of life sciences...
  9. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...cloned by two separate labs (Bird et al., 1988; Huston et al., 1988). scFvs are the smallest unit of an.... https://doi.org/10.1371/journal.pone.0189964  Huston JS, Levinson D, Mudgett-Hunter M, Tai MS, Novotny...
  10. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...of such targets include transcription factors, histones, and DNA repair enzymes. Note that when using ...stepwise decrease in fluorescence intensity in a (histogram) flow plot (Figure 4b). Through this method, differences...
  11. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...respectively. H, A, and W describe the shape of a histogram, which graphically represents the time and intensity...graph of photocurrent vs. time. The resulting histograms is described through maximum current (H), the...
  12. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...edition. Cambridge university press, 1988. AAS: Historical Trends in Federal R&D NJ.com: Rush Holt: Lawmakers...affected by Trump’s immigration ban Studies in History and Philosophy of Science Part: Democratic values...
  13. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ... al is a PCR-based approach for generating a customizable comprehensive mutagenesis library that’s ready...sites in the same orientation as the gene or use custom gene synthesis or site directed mutagenesis to ...
  14. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...However, ART is not an HIV-1 cure. In patients who stop ART, viral levels will soon skyrocket back to pre-treatment...HIV-infected reservoir, researchers have previously used histone deacetylase (HDAC) inhibitors to increase transcription...
  15. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ... to the new genomic locus. Image courtesy of Christopher Fell, PhD. After minimizing the system's...enhancer-AAV constructs are now available as in-stock viral preps using the systemic PHP.eB capsid, to...
  16. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 2:15 p.m.
    ...Availability of AAV serotypes at Addgene. Serotype In-stock viral prep Packaged on Request AAV1  ✓ ✓ AAV2... Data Hub AAV Packaged On Request Browse all in-stock AAV preps Browse all AAV plasmids ...
  17. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...ICC) when the sample is cultured cells or immunohistochemistry (IHC) with tissues), where an antibody binds....3001161 McLaughlin, K. (2019). Multiplexing Immunohistochemistry. Materials and Methods, 9. https://doi.org...
Showing: 361 - 380 of 611 results