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... were lots of fluorescent proteins made from jellyfishes and corals, but all of them fluoresce outside...

...long vesicles have hollow interiors and a protein shell that excludes water but is gas permeable. These ...

...different strands to create a DSB, but this can be accomplished with either a PAM-in or PAM-out orientation....

...antibodies and affinity reagents. But for a quick refresher, some of the advantages of sdAbs over conventional...

..., and VP3, which together form the outer capsid shell that protects the viral genome. The cap gene is ...

...substitutions don’t fare much better: they largely abolished cleavage by both Cas9 and Cas12 (Kim et al., 2020...

...Chemogentic and optogenetic technologies have pushed the boundaries in neuroscience by granting targeted...

...Isaacman-Beck J, Paik KC, Wienecke CFR, Yang HH, Fisher YE, Wang IE, Ishida IG, Maimon G, Wilson RI, Clandinin...

...Originally published Jan. 14, 2014 and updated Apr. 14, 2020. Any newcomer who joins a molecular biology...

...sequence information contributed by the depositor, published backbone sequences, and any known sequences for...

... Just over a month ago I finished up my PhD in Biological and Biomedical Sciences at Harvard University...

...Addgene Blog to follow the entire series as it is published. For more reading try The Discipline of Teams ...

...Addgene Blog to follow the entire series as it is published. Good introductory reference books on managing...

...backbones that have been designed, tested, and published by academic scientists. To help you find the vector...

...community. In the long term, the research project establishes your professional brand. It offers colleagues...

..., interests and passions is another great way to shed light on what paths to pursue. Spend some time really...

...nts, and is thermostable between 31-100°C. They published their finding in a bioRxiv article and in PNAS...

...oligomerization Seeing red: simple GFP photoconversion Light sheet fluorescence microscopy Resources on Addgene.org...

...Originally published Feb 6, 2014 and last updated Nov 10, 2020. Now that we know all about antibiotic...

...Bandrowski et al., 2016). It’s also been well established that recombinant antibodies are excellent for...