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We narrowed to 809 results for: TIM;

Showing: 361 - 380 of 809 results

  1. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    Published
    Dec. 2, 2019, 2 p.m.
    ...Addgene! Since the development of BioID and APEX, optimized versions of BioID and APEX have been developed...promiscuous biotin ligase The Roux Lab in 2016 optimized BioID to create BioID2, a smaller and improved...hypothesized that the low sensitivity was due to suboptimal folding or stability. Thus they conducted multiple... significantly greater than miniTurbo at earlier time points but miniTurbo exhibits less labeling prior...

  2. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...cassette. This leaves a “scar” in the chromosome, sometimes interfering with expression of surrounding genes...technique, and is less prone to off-target mutations. Ultimately, successful allelic exchange requires two sequential...mutants, but it frequently requires considerable optimization to ensure strong counter-selection. Anecdotally...magenta. This is particularly valuable when first optimizing conjugation in strains that don’t conjugate that...

  3. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ... and Lichtman estimate that Brainbow’s colorful labeling has decreased the mapping time for a given section... were limited due to low resolution or were very time-intensive, necessitating the development of a new...is truly fitting for this colorful technology. Optimizing the system: Brainbow-3 Although Brainbow provided...

  4. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...behaviors, but little is known about the order or timing of these factors. Recombinase-based state machines...reaction can only be performed on one register at a time. If a particular application makes it difficult ...machine design space is highly degenerate and often times there are multiple register designs that may be ...? We tested a two-input (ATc and Ara) RSM over a time series of increasing input exposure lengths and ...

  5. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...inconclusive results, erroneous conclusions, and wasted time. In fact, many have called out antibodies as major...and so this approach may not be best if you are ultimately planning to use your antibody to detect endogenous... proceed with confidence.  Share your results Ultimately, like all of science, validation experiments ...could work great in another context! So the next time you test an antibody (perhaps one from Addgene!)...

  6. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...with linear DNA as repair templates which reduces time to generate edits to only four days (Paix et al.... rapid. This stringent selection allowed me to optimize a range of editing parameters: oligo repair templates...Farboud et al., 2019). The Meyer lab also determined optimal PAM orientation for editing using two Cas9 targets...Resources Learn about gRNA design and gRNA optimization for your CRISPR experiment ...

  7. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...2015). Meanwhile, Sanger sequencing is laborious, time consuming and cannot be applied to heterogenous ...multiplexing) Requires large cell population, senstive to time since cell fixing HTGTS Not reported Cell-based...sequencing techniques, please see “Methods for Optimizing CRISPR-Cas9 Genome Editing Specificity” by Tycko...Vic E. Myer, and Patrick D. Hsu. "Methods for Optimizing CRISPR-Cas9 Genome Editing Specificity." Molecular...

  8. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...as confusing as reading Shakespeare for the first time. The discovery and application of CRISPR-Cas9 kicked...bacterial and archaeal immune systems. During this time, scientists discovered many related immune mechanisms... only recently that researchers began building optimal plasmids to harness the power of Class 1 systems... your goal, experience, hypothesis, and even the time and money available. The breadth and diversity of...

  9. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...synthesized and degraded within the cell, so at any given time, an FP-chimeric protein may be at any one of many...quenching”). The photoswitch can occur multiple times by activating with two distinct wavelengths of light...possible excitation lasers) it is also important to optimize your activation protocol for your particular set...heavily on the same fluorophore being imaged multiple times in order to reconstruct an image. The best PA-FPs...

  10. New Videos: Addgene Lab Tips

    Type
    Blog Post
    Published
    Feb. 15, 2018, 2:41 p.m.
    ...distributed over 800,000 plasmids since 2004. In that time, our lab has gotten a lot experience in processes...tip #1: Glove trick Putting on lab gloves can sometimes be annoying. To make it easier, try this quick...a single tube and simply flick the tube several times. This will help ensure ensure equal distribution...

  11. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...is recognizing the PAM on DNA, the CTD is also sometimes called the PAM-interacting (PI) domain. The guide...al., 2013). As mentioned above, however, Cas9 sometimes tolerates mismatches in the next 8-10 bases after...and the DNA sequence is recognized, it’s finally time to cut! The HNH domain, which cuts the target DNA...Shima, T., Nureki, O., & Uemura, S. (2018). Real‐time observation of flexible domain movements in CRISPR–Cas9...

  12. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    Published
    Nov. 6, 2018, 1:12 p.m.
    ...contributed by Kusumika (Kushi) Mukherjee. The ultimate goal in the field of regenerative medicine is ...iPSCs into the cell type of choice. This step is sometimes also referred to as “directed differentiation....39]. In 2000, Ferber et al. showed for the first time that mouse liver cells could be transdifferentiated...Partial rearrangement from one cell type to another Time and resources More (due to additional pluripotency...

  13. Podcast: A Malawian Professor's Path to Biotech Research

    Type
    Blog Post
    Published
    May 24, 2016, 2:30 p.m.
    ...Thoughts on his time in Boston and Outro We'd like to thank Dr. Kwapata for taking the time to talk to us...across the globe. Listen here! Podcast Breakdown (Time: Topic) 0:00-1:00 : Intro 1:01-3:48 : Kingdom Kwapata's...

  14. #DeckTheLab with Tinsel and Test Tubes, Fa La La La La La La La La

    Type
    Blog Post
    Published
    Dec. 2, 2021, 2:15 p.m.
    ...here at Addgene HQ in Massachusetts that means it’s time for near-freezing temperatures, our first of the...decorating contest! Our #DeckTheLab contest is a time for scientists worldwide to show off their decorating...little more festive this holiday season! It is that time of the year again: miniature #Christmas tree goes...

  15. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    Published
    Sept. 1, 2016, 2:30 p.m.
    ...graduate student life. Senior graduate students can sometimes be elusive or have a certain mystique that makes...Listen to the interview here! Podcast Breakdown (Time : Topic) 0:00 - 0:28 : Niroshi and Ben describe ...We'd like to thank Niroshi and Ben for taking the time to sit down and talk to us and wish them the best...

  16. Plasmid Preps: Different Purity, Different Quantities, Different Uses

    Type
    Blog Post
    Published
    June 16, 2020, 1:25 p.m.
    ...origin. The type of cloning approach you choose ultimately will influence the quantity of DNA that is required...viability, reduces transfection efficiency, and ultimately affects the quality and outcomes of experiments...plasmid DNA, so that you can decide what will be the optimal type of plasmid preparation in terms of quantity...

  17. Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors

    Type
    Blog Post
    Published
    March 17, 2021, 2:40 p.m.
    ...Addgene’s compensation decisions. It was a great time for me to join the committee because I had recently...community? Access to plasmids which gives people more time to focus on their research.  How will your prior...that I’m not working so that I’m available to spend time caring for my mother. For fun, I enjoy pilates, ...

  18. Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies

    Type
    Blog Post
    Published
    Feb. 26, 2019, 1:40 p.m.
    ...meetings, and finding the best way to organize your time. At a conference, you could be honing your science...can't wait for opportunities to fall in your lap. Sometimes, in order to do the thing you love, you have to...  Break the process down into steps and set aside time during the week to tackle each one.  Get the toughest...

  19. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    Published
    May 1, 2014, 8:23 p.m.
    ...you need the right experimental materials. Who has time for paperwork? Here’s why a Material Transfer Agreement.... Here’s how Addgene helps: For Providers: One-time Deposit: Simply deposit with Addgene (it’s FREE!...Keeping: Addgene will let you know exactly how many times your materials have been requested and by whom. ...

  20. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    Published
    Jan. 23, 2014, 3:23 p.m.
    ...technology with the Golden Gate method. For the first time, it was possible to quickly and efficiently assemble... in subsequent rounds, dramatically reducing the time required to generate multi-construct transgenic ... half a year,” in comparison to more tedious and time-consuming traditional cloning methods, Lohmann said...
Showing: 361 - 380 of 809 results