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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 738 results for: TRI

Showing: 361 - 380 of 738 results

  1. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...lentiviral vectors, the lentiviral genome is distributed across multiple plasmids. For simplicity, we’...settings, as evidenced by retroviral gene therapy trials for X-linked severe combined immunodeficiency (... (SCID-X1) in the early 2000s. These trials unfortunately resulted in several patients developing T-cell...

  2. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...committees. Having successfully navigated the trials and tribulations of the process, I’ve provided guidance ...ability to interview and sell a vision for your contribution to a Department's/Institution's success is the...

  3. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...Originally written by Marcy Patrick and Mary Gearing on Mar 12, 2015; updated by Christina Mork, Jul ...2023. DNA damage drives genome instability and contributes to cancer, premature aging, and other harmful...catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA. Richardson CD, et al. Nat Biotech....

  4. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ... of Biotin Attachment to Proteins; Symposium: Nutrition, Biochemistry and Molecular Biology of Biotin”...forgotten vitamin.” American Journal of Clinical Nutrition 75.2 (2002): 179–180. PubMed PMID: 11815306. 4...technology to improve cell adhesion on nanofibrous matrices.” Journal of Nanobiotechnology 13.1 (2015): 1–...

  5. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...This post was contributed by Kyle Hooper at Promega. Researchers have been sharing plasmids ever since...Intracellular Calcium Sensor Yang, J. et al. (7) wished to trigger intracellular Ca++ release with an optogenetic ...specificity High sensitivity Easy expression Gives ratiometric data The result was a fusion protein called ...

  6. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...iGluSnFR3, iGluSnFR4f, and iGluSnFR4s. C) Spike-triggered averages for single action potential-evoked axonal...promoter, tag, polyA site) are flanked by unique restriction sites so you can swap sequences effortlessly....vectors targeting specific classes of cortical and striatal interneurons (Furlanis, Dai, Leyva Garcia et al...

  7. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...This post was contributed by guest blogger Greg Lohman, a biochemistry researcher at New England Biolabs...joining for recombinant plasmid construction (restriction cloning), adaptor ligation for next generation... can be achieved through a combination of the intrinsic selectivity of the ligase active site and careful...

  8. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...with fluorescent derivatives of its substrate trimpethoprim (TMP-tag) (Miller et al., 2005). Although this...Benzylguanine (BG) Many Easy eDHFR 159 Trimpethoprim  (TMP-tag) Few Easy HaloTag 295 ... V. W. (2005). In vivo protein labeling with trimethoprim conjugates: A flexible chemical tag. Nature ...

  9. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 15, 2017, 1:24 p.m.
    ...fluoresce, and then passing the cells through an electric field to deflect them into appropriate containers...understanding the key roles of immune cells in triggering an immune response. New fluorescent proteins ... PubMed PMCID: PMC5024581. 15. Celli, Susanna, Béatrice Breart, and Philippe Bousso. "Intravital two-photon...

  10. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ... This post was contributed by guest blogger Fredrik Wermeling, leader of a research group at the Centrum...to ensure that each gRNA has a good chance to contribute to the screen. In the end, this results in an...could majorly accelerate progression into clinical trials. Secondary screens  If you have generated a list...

  11. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...systems are less popular in the lab. This can be attributed to the multi-Cas effector, Cascade. Developing...Developing tools using a Cascade complex is tricky, as you need to ensure every unit of the complex expresses... Tolstoy, I., Johnson, D. B., Koonin, E. V., & Quatrini, R. (2021). Evolution of Type IV CRISPR-Cas Systems...

  12. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...This post was contributed by guest blogger Stephanie Hays, a researcher at the University of California...age live in a state with at least two abortion restrictions that conflict with scientific evidence. We need...promote harmful policies that benefit polluting industries.In March, three climate scientists and an economist...

  13. CRISPR 101 eBook...en español

    Type
    Blog Post
    Published
    Jan. 18, 2024, 2:15 p.m.
    ...audiencia internacional, incluido trabajando con distribuidores de todo el mundo para ayudar a aumentar el ...international audience, including working with distributors across the world to help increase global access...

  14. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    Published
    Feb. 1, 2024, 2:15 p.m.
    ... Addgene will then apply for the license for distribution to the requester’s organization. A non-refundable...for rabies materials may be required for some countries and may not be required for others. You will be...

  15. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    Published
    Oct. 17, 2024, 1:15 p.m.
    ...of all our depositors here at Addgene — your contributions make the repository what it is. So we’re thrilled...crystal structures demonstrating “the structure is strikingly similar to the design model at atomic resolution...

  16. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    Published
    June 13, 2016, 7:08 p.m.
    ... to develop resources for your community! To contribute plasmids made in your lab, see www.addgene.org... a colleague who would benefit from our free distribution services for their popular constructs, why not...

  17. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ...will vote on the entries, select up to three winners, and reveal the winning entries on Twitter and Instagram...

  18. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ...tips you give to every new lab mate? Do you have a trick to get the highest yield out of your minipreps? ...love to have you write for us! Click Here to Contribute to the Addgene Blog Possible guest topics Some...

  20. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    Published
    Sept. 25, 2018, 2:39 p.m.
    ...’ve enjoyed seeing scientists sharing their contributions on social media as well the excitement amongst...community. For example, we started a new service distributing ready-to-use viral preps in 2016 and we just...
Showing: 361 - 380 of 738 results