We narrowed to 649 results for: VARS

...developed a modular suite of plasmids (pGT) with various replicative origins that can be used for broad ... host-range of the plasmids and suitability of various antibiotic resistance markers, promoters, and inducible...

...primary antibody without interfering with the hypervariable region, where an antibody binds to its target...signal amplifiers, the flexibility of their use varies across applications. Western blots, for instance...

...researchers feel they lack formal training in a variety of transferable skills. At Addgene, we've set out...to apply the scientific method to problems in a variety of contexts.  How do you develop problem solving...

...polyclonal antibodies is their batch to batch variability. Once an animal has undergone a terminal bleed...antibody instead. Otherwise, you might experience variability in the strength of staining and background noise...

...purification. There is one simple method that works, and variations to that method. A major hurdle to developing ...isolate RNA from 20+ hours to around 4 hours, and variations on this no-kit method are still widely used today...

...   Ask the learners themselves for feedback in a variety of ways, like having them restate or summarize ...you intended (it happens to us all!) and offer a variety of options to make your content more accessible...

...interest. Thanks to the tremendous efforts made by various research teams to build and constantly improve ...integrated, we look forward to the realization of a variety of perturbations and applications in functional...

...for identifying, categorizing, and resolving a variety of QC issues. This process ensures that any potential...making the same decisions when analyzing the wide variety of discrepancies encountered in plasmids deposited...

...overcome this, lentivirus can be concentrated by a variety of methods such as ultracentrifugation, centrifugation...Finally, choice of concentration method depends of a variety of factors such as resources available, cost and...

...that by demonstrating GdnHCl chemical rescue of a variety of disease-linked Arg-mutant proteins in the lab...then mutagenize the NFIX gene and test whether variants of unknown significance (VUSs) that are frequently...

...that use CRISPR/Cas9 to endogenously tag a wide variety of genes with GFP. These plasmids are available...gene of interest. Researchers have developed a variety of endogenous gene tagging systems. Addgene’s CRISPR-based...

... either 3-base or 4-base overhangs, and with a variety of different enzymes and cycling conditions (see... to 49% fidelity, with significant lot-to-lot variation. Additionally, this assembly, which used only ...

... deliver Cas9 variants, such as a transcriptional activator dCas9-VPR, if these variants are fused to ...

... homozygous at nearly all loci. This reduces variability in genotype and phenotype and helps improve the...the cells in which Cre is expressed.  Several variations of this technology can be used to customize the...

... Yuhai Cui Red mScarlet Split-wrmScarlet variant of mScarlet Split-wrmScarlet and split-sfGFP: tools...–10) and sfCherry2(11), also photo-activatable variant PAsfCherry2(1–10) Improved split fluorescent proteins...

...process and includes Picogreen quantification of various intermediate steps, a sizing electrophoretic gel... the pool but instead selectively sequence the variable portion of the plasmid in the pool - ex. the gRNA...

... these modules (linker length can and should be varied as it can have important implications for the fusion... Various Addgenies...

...were transduced with H2B-FT virus and grown in varying concentrations of IL-3. Cells grown in high concentrations... Various Addgenies...

...CRISPR activation uses dCas9, a CRISPR protein variant lacking its endonuclease ability, to bind to genes...factors to increase gene expression. CRISPRa methods vary in their transcriptional activators: some methods...

... CRISPR-Cas technology is constantly evolving. Variants of Cas proteins can be used for genome editing...out this post. Learn how to Choose the Best Cas9 Variant for Your Next Experiment Read more about Optogenetic...