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We narrowed to 519 results for: abo.2

Showing: 361 - 380 of 519 results

  2. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ...available to the community. We now offer AAV9 for 2 more constructs from Christopher Harvey’s lab. These...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

  3. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...biology 14.6 (2004): 757-764. PubMed PMID: 15582400. 2. Tomkinson, Alan E., et al. "DNA ligases: structure... And what is ligase fidelity anyway? Let’s talk about it. DNA ligases are enzymes that seal breaks in ...to accelerate characterization of nucleic acid metabolic enzymes." Nucleic acids research 44.2 (2015): ...

  4. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ... remember that a resume is typically no more than 2 pages long. Write your resume for the job posting ...patent application processes? How about facilitating a collaboration between research groups? Perhaps ... a good job summarizing what a resume should be about. With a CV, information is usually presented chronologically...developed through your research? Don't simply write about the research you did. Emphasize the skills. If you... structures, then you might know a thing or two about computer modeling. Make sure to focus on these skills...the job you are applying for, in which case talk about your research, as an understanding of the topic ...you some advantage over other candidates. Write about your transferable skills Some of the skills you ...

  5. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...biosensors, protein evolution lineages, 2-photon spectra, more elaborate spectrum analysis tools, and a better...collate some of the current “best” FPs, and I collaborated with him on a web-based interactive visualization...deal more information that one might like to store about FPs than is currently represented at fpvis (complete...thousands of scientists routinely using and thinking about FPs, it would be great to have a centralized, community-editable...resource for storage and dissemination of information about FPs. FPbase.org FPbase is a project written in ...comprehensive community resource for information about fluorescent proteins.  It was designed with the ... there is plenty of room for improvement and elaboration.  Current projects include incorporation of biosensors...

  6. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    Published
    Oct. 20, 2020, 1:15 p.m.
    ...peer-mentoring groups at Addgene have gone virtual!   2. Practice public speaking For almost any science career...mentoring group (see above). However, now that we are all so good with video calls, how about trying your hand...blog or podcast, initiated and maintained a collaborative project, served on the board of a professional...appointments with people all over the world. Think about contacting alumni of your lab or university or use...suggest you speak with. See this post that’s all about informational interviews, for more details on how...progress on both building relationships and thinking about future career options. 5. Write a grant - even if...

  7. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ...PMID: 30083608. PubMed Central PMCID: PMC6070312. 2. Stark, Jessica C., et al. "BioBits™ Bright: A fluorescent...student at MIT. While I had always enjoyed learning about biology in high school, it wasn’t until I started...college that I really fell in love with it. Something about being able to actually hold all those seemingly ...seemingly abstract biological reactions that I learned about from textbooks just made everything click in my head...students build circuits in physics class to learn about electronics or mix chemicals together in chemistry...a power source, but now you don’t have to worry about operating the rest of the car as well. Similarly...with us! We’ll work with you to get the word out about your plasmids and their potential educational uses...

  8. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    Published
    April 19, 2017, 3:25 p.m.
    ...from a single Illumina MiSeq® run. As shown in Fig. 2, below, differences in input concentration across ...”) of DNA, have changed our collective thinking about the feasible size and scope of projects in many ...next generation (NGS) sequencing have prevented laboratories from using these methods for routine processes...daunting for large numbers of samples. As mentioned above, one of the primary technical benefits of plexWell...1ng – 100ng) are normalized to two-fold variation above and below mean read count per sample, and the amount...NGS readout also reveals additional information about the presence of non-plasmid DNA or heterogeneous...   Check out the SeqWell website to learn more about our plexWell kits and services! Many thanks to Joe...

  9. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    Published
    July 21, 2020, 1:15 p.m.
    ...automated. Unlike gel electrophoresis, you only need 1-2 ul of sample and the run time is just a few minutes...distinguish between DNA and RNA. See our protocol about using UV absorbance to quantify DNA Fluorescence...absorbance based methods, this method doesn’t tell you about contaminating proteins or chaotropic salts. Capillary... instruments are available. Some last reminders about quantifying DNA In choosing your DNA quantification...Additional resources on the Addgene blog Read more about molecular biology protocols and tips Find the best...best way to elute and store your plasmid DNA Learn about the different grades of plasmid DNA preps Resources...

  10. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...created for the Brunello library is shown in Figure 2. You can use a small PCR product because the plasmids... and we thought it would be a good time to talk about the amplification and verification processes we ... DNA. These reactions should allow us to obtain about 100 to 1,000 bacterial colonies per individual plasmid...from the count_spacers.py file from Feng Zhang’s laboratory. There are a number of other analysis scripts...you have any tips, input, or stories of interest about library use, let us know in the comments! Additional...NGS Sequencing to Validate AAV Preps Learn More about Pooled CRISPR Libraries Resources on Addgene.org...

  11. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ... and whether I would be able to carry out a yeast-2-hybrid library screen; instead I thought they would...for Dummies”. The fastest way to learn something about marketing would be to start reading a broad simple...introduction to the financial world. It teaches you about selling products which is an important part of the...Seán on twitter @Macfhearraigh.    Read more posts about Scientific Career Development ...

  12. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ... ligands can elicit varying degrees of β-arrestin-2 recruitment to receptors, indicating their agonistic...fluorescent labels. In Nature Methods (Vol. 19, Issue 2, pp. 149–158). Nature Research. https://doi.org/10.1038... A., Purkayastha, N., Flad, K., Schmidt, M. F., Tabor, A., Gmeiner, P., Hübner, H., & Weikert, D. (2020...

  13. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...two options to begin your plasmid deposit. Figure 2: Choose to submit plasmid(s) online or by using a ...format. Figure 6: Add sequence, maps, and files. Step 2: Gene and Insert *Note: This page will not appear ...annotate on this page. Figure 7: Enter information about the gene/insert(s). Step 3: Cloning Information ...

  14. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    Published
    June 2, 2020, 1:15 p.m.
    ...our SPARC blog post for more details. Figure 2: Dense (c), intermediate (d), and sparse (e) labeling... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.  ...non-overlapping cells. Until now, tools for doing this were labor-intensive and required using manipulations such ...

  15. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    Published
    April 23, 2020, 1:15 p.m.
    ...containing complementary polynucleotide chains. J Virol 9(2): 394-396. Lecomte E, Tournaire B, Cogné B, Dupont...share the procedures and Python scripts with laboratories and viral vector cores routinely producing AAV...on Addgene.org Find AAVs at Addgene Learn more about Addgene's viral service Find protocols for working...

  16. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...is the most charged, with 5 negatively charged and 2 positively charged amino acids. FLAG tag has been ...Tags have a lot of major benefits – as discussed above – but there are a few instances when they should... hindrances, and destabilization can be brought about by the tag so it’s important to validate tagged ...

  17. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...Bacterial Cell Envelope. Cold Spring Harb Perspect Biol. 2(5) a000414 (2010). Cleveland Clinic. Gram Stain. https... it can be used in a lab? Here we’ll be talking about the ins and outs of this classification system, ...The classification system is also useful in a laboratory setting. When designing a plasmid to be inserted...

  20. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ...delivering an AAV construct expressing channelrhodopsin 2 (ChR2), a blue light sensitive cation channel, fused...I often wonder about the gut-brain axis (admittedly for self-serving reasons – I want to understand my...
Showing: 361 - 380 of 519 results