We narrowed to 530 results for: abo.2

...light sensing proteins. Furthermore, during the first 2 weeks of life, larvae already exhibit distinct behaviors... posts about viral vectors Subscribe to the Addgene blog Resources on Addgene.org Learn about our ready-to-use...

...access this tool, set up an IDT account. Figure 2: Addgene's Analyze Sequence tool.   DNA sequence...particular tasks, but they tend to be scattered about the internet. To help you keep your virtual molecular...other applications Primer design tools Many of the above-listed plasmid mapping tools have built-in primer...

...affinity and specificity. Trends in Immunology, 29(2), 91–97. https://doi.org/10.1016/j.it.2007.11.004 ...phagocytosis.  Other functions We’re still learning about Fc effector functions and all that antibodies can...isotypes and Fc receptors that we don’t know much about yet. Who knows what kinds of functions they may ...

...majority of deposited materials to study SARS-CoV-2 would be fully open and available to industry requestors...Our work accelerates and enhances productive collaborations as well as provides visibility for grant-driven...research is accelerating essential research in laboratories all around the world." We thank the Chan Zuckerberg...

...make it easier to study these mutants.   Fig. 2: (A) Betacyanin is produced from tyrosine by the activity... is difficult for new members of the lab or collaborators unfamiliar with these mutant plants. Using RUBY...resistant disease. (RUBY-Red: You can’t beet it!)   About the Author  Andrew Willoughby is a graduate student...

...interferon gamma (IFNγ) - and eliminate cancer cells. 2. Equilibrium - In a process analogous to the development... 35. Academic Press, 2015. PubMed PMID: 25818339. 2. Curiel, Tyler J., et al. "Specific recruitment of...potential beneficiaries. Collaborative therapy For the most efficient outcomes, collaborative therapies must be... of the tolerance-producing antigens enunciated above. Finally, signals released from the microenvironment...are in their clinical trial phase. In all of the above therapeutics, techniques like genome engineering...are beginning to see the fruits of many years of labor with up and coming immunotherapy treatments. As ...clinic, physicians and researchers are learning much about how treatment regimens can be improved and how the...

...establishing the dynamic range of your FRET assay (Figure 2). These labeling methods are freely interchangeable...PMID: 20161833. PubMed Central PMCID: PMC2802762. 2. Dumas, Anaëlle, et al. "Designing logical codon reassignment–Expanding...environment? If these are pressing issues in your laboratory, then FRET experiments are a viable biophysical...to consider these cases separately when talking about the tools that are available. Let’s consider the...that, if you make an FP fusion, you are inserting about 20-25 kDa of protein mass into your protein and ...together The FRET donors and acceptors described above can be paired together to measure a wide range of...of your protein that can lead to novel insights about its biology and its behavior. See the table below...

... master's degree prepared them for their career.  2. Are you looking for career advancement and growth... Resources Resources on the Addgene blog Learn about career planning resources What Do I Do Now? Academic...

...development and therapy. Nat Rev Drug Discov (2017) 16(2):89-100. DOI: 10.1038/nrd.2016.238 Additional resources...type of edits can be made in the contexts outlined above. Layers of control have also been added to the CRISPR...

...method relies on the fluorescence ratio between the 2 fluorophores, rather than on the fluorescence intensity...CRISPR library from Alec Kimmelman targets mouse metabolic genes with ~6 gRNAs per gene.  CRISPR-Act3.0, ...

...available to the community. We now offer AAV9 for 2 more constructs from Christopher Harvey’s lab. These...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

...that underlie a particular phenotype. Figure 2: Schematic of CRISPR knockout screening with a pooled...variations in the spike protein, teaching us more about viral evolution and antibody binding. Figure...

...biology 14.6 (2004): 757-764. PubMed PMID: 15582400. 2. Tomkinson, Alan E., et al. "DNA ligases: structure... And what is ligase fidelity anyway? Let’s talk about it. DNA ligases are enzymes that seal breaks in ...to accelerate characterization of nucleic acid metabolic enzymes." Nucleic acids research 44.2 (2015): ...

... remember that a resume is typically no more than 2 pages long. Write your resume for the job posting ...patent application processes? How about facilitating a collaboration between research groups? Perhaps ... a good job summarizing what a resume should be about. With a CV, information is usually presented chronologically...developed through your research? Don't simply write about the research you did. Emphasize the skills. If you... structures, then you might know a thing or two about computer modeling. Make sure to focus on these skills...the job you are applying for, in which case talk about your research, as an understanding of the topic ...you some advantage over other candidates. Write about your transferable skills Some of the skills you ...

...biosensors, protein evolution lineages, 2-photon spectra, more elaborate spectrum analysis tools, and a better...collate some of the current “best” FPs, and I collaborated with him on a web-based interactive visualization...deal more information that one might like to store about FPs than is currently represented at fpvis (complete...thousands of scientists routinely using and thinking about FPs, it would be great to have a centralized, community-editable...resource for storage and dissemination of information about FPs. FPbase.org FPbase is a project written in ...comprehensive community resource for information about fluorescent proteins.  It was designed with the ... there is plenty of room for improvement and elaboration.  Current projects include incorporation of biosensors...

...peer-mentoring groups at Addgene have gone virtual!   2. Practice public speaking For almost any science career...mentoring group (see above). However, now that we are all so good with video calls, how about trying your hand...blog or podcast, initiated and maintained a collaborative project, served on the board of a professional...appointments with people all over the world. Think about contacting alumni of your lab or university or use...suggest you speak with. See this post that’s all about informational interviews, for more details on how...progress on both building relationships and thinking about future career options. 5. Write a grant - even if...

...PMID: 30083608. PubMed Central PMCID: PMC6070312. 2. Stark, Jessica C., et al. "BioBits™ Bright: A fluorescent...student at MIT. While I had always enjoyed learning about biology in high school, it wasn’t until I started...college that I really fell in love with it. Something about being able to actually hold all those seemingly ...seemingly abstract biological reactions that I learned about from textbooks just made everything click in my head...students build circuits in physics class to learn about electronics or mix chemicals together in chemistry...a power source, but now you don’t have to worry about operating the rest of the car as well. Similarly...with us! We’ll work with you to get the word out about your plasmids and their potential educational uses...

...from a single Illumina MiSeq® run. As shown in Fig. 2, below, differences in input concentration across ...”) of DNA, have changed our collective thinking about the feasible size and scope of projects in many ...next generation (NGS) sequencing have prevented laboratories from using these methods for routine processes...daunting for large numbers of samples. As mentioned above, one of the primary technical benefits of plexWell...1ng – 100ng) are normalized to two-fold variation above and below mean read count per sample, and the amount...NGS readout also reveals additional information about the presence of non-plasmid DNA or heterogeneous...   Check out the SeqWell website to learn more about our plexWell kits and services! Many thanks to Joe...

...created for the Brunello library is shown in Figure 2. You can use a small PCR product because the plasmids... and we thought it would be a good time to talk about the amplification and verification processes we ... DNA. These reactions should allow us to obtain about 100 to 1,000 bacterial colonies per individual plasmid...from the count_spacers.py file from Feng Zhang’s laboratory. There are a number of other analysis scripts...you have any tips, input, or stories of interest about library use, let us know in the comments! Additional...NGS Sequencing to Validate AAV Preps Learn More about Pooled CRISPR Libraries Resources on Addgene.org...

...automated. Unlike gel electrophoresis, you only need 1-2 ul of sample and the run time is just a few minutes...distinguish between DNA and RNA. See our protocol about using UV absorbance to quantify DNA Fluorescence...absorbance based methods, this method doesn’t tell you about contaminating proteins or chaotropic salts. Capillary... instruments are available. Some last reminders about quantifying DNA In choosing your DNA quantification...Additional resources on the Addgene blog Read more about molecular biology protocols and tips Find the best...best way to elute and store your plasmid DNA Learn about the different grades of plasmid DNA preps Resources...