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We narrowed to 811 results for: cha

Showing: 361 - 380 of 811 results

  1. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ... electrical current recorded by the instrument changes. Base-calling software can then identify the nucleotide...nucleotide that passed through based on these characteristic drops. Figure 3: Overview of Nanopore... up the pace Our QC team constantly faces the challenge of keeping up with the large amount of QC analysis...., & Coulson, A. R. (1977). DNA sequencing with chain-terminating inhibitors. Proceedings of the National...

  2. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    Published
    Sept. 13, 2018, 1:55 p.m.
    ...effects. The flaws of RNAi screens are well characterized and strategies exist to control for these faults...also reproduced several previously observed characteristics that influence gRNA off-target activity: 1)...23394947. Pubmed Central PMCID: 4299491. 5. Bassik, Michael C., et al. "A systematic mammalian genetic interaction...available from Addgene Other Resources Watch Dr. Michael Bassik’s lecture on “Multiplexing with CRISPR Screens...

  3. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...Design). This modular approach combines an interchangeable set of fusogenic and targeting components to...where the fusion and targeting components are interchangeable. Image from Strebinger et al., 2023, reused...local proteome, but some applications have been challenging or inaccessible. The Ting lab recently made two...They demonstrated LOV-Turbo in neurons, in pulse-chase experiments, and more. Find LOV-Turbo plasmids here...

  4. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...the chances of a cell being infected by only one viral particle, but it also increases the chances that...presentation deceive you; pooled libraries can be quite challenging to use! In this post, I’ll walk you through how...virus. Let’s start there!     Figure 1: Flowchart showing the process for using pooled lentiviral...

  5. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type
    Blog Post
    Published
    Dec. 14, 2017, 2:08 p.m.
    ...of reprogramming (or “Yamanaka”) factors, they changed the landscape of regenerative medicine. Their work...viral vectors are preferred. This is to avoid the chances of insertional mutagenesis, which is the integration...replacing C-MYC with L-MYC [10, 11]. While the mechanisms underlying the enhancement of iPSC generation...Induced Pluripotent Stem Cells (iPSCs): Benefits, Challenges and the Road Ahead." Journal of Alzheimer's disease...

  6. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...expertise with that of the company. To increase your chances at successfully applying for a position, you should... in a technical support role. What general characteristics do jobs in technical support have? There are... less time consuming and/or less technically challenging. Alongside there is a second-line team that takes... ways to communicate with customers. Online live chat is a convenient alternative for asking a quick question...

  7. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...protected by Cas9. This is similar to the proposed mechanism for engineered pegRNAs (epegRNAs), which add a...of mouse monoclonal antibodies generated and characterized by the Geiss Lab in their effort to fill a void...cells. Plus, Voltron2’s fluorescence is tunable by changing the dye that becomes conjugated with its HaloTag...gene expression regulation, enhancing both the mechanisms of delivery and the potential for therapeutic...

  8. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...Understanding the characteristics of natural plasmids and how they function in nature can inform us on...often have a restriction barrier as a defense mechanism, and they can degrade foreign bacteria through...expressed. For example, scientists can incorporate interchangeable antibiotic resistance cassettes (Prior et al...Schmidhauser, T. J., Ditta, G., & Helinski, D. R. (1988). Chapter 15—Broad-Host-Range Plasmid Cloning Vectors for...

  9. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...following changes. In pCas9, we 1) replaced the promoter of gRNA-pMB1 with PrhaB, 2) changed the replicon...same year. Her research is focused on the escape mechanism of genome editing in microorganisms which could...cells to understand the genetic and biochemical mechanisms behind their distinct phenotypes and to design...

  10. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    Published
    Feb. 18, 2020, 2:15 p.m.
    ...General Meeting seven other times I had subsequently changed fields and my cohort of friends and colleagues ...evolutionary lineages share genes that can improve chances of survival for an individual, and eventually, ... cells regulate gene expression in response to changes in cell density. At the mixer, they meet other ...Dill-McFarland, Lisa Gorski, Mark O. Martin, Maureen Berg, Michael D. L. Johnson, Tiffany Lowe-Power, Eric Griffis...

  11. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...incentivize good mentorship: AMA mechanics: Be prepared! The mechanics of doing the AMA were interesting...Getting on a good science career path can be challenging these days, but for many of us, scientist is ...Science through Video with the Addgene Youtube Channel Check Out Vidoes of Gender & Science from LabLinks...

  12. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...for researchers looking to generate long-lasting changes in cells. However, for some applications, integration...numerous variants have been developed with different mechanisms to inactivate the integrase.  The IDLV life cycle...construct has the D64V mutation, a single amino acid change (from aspartic acid to valine at position 64) which...Kempski, H., Brugman, M. H., Pike-Overzet, K., Chatters, S. J., de Ridder, D., Gilmour, K. C., Adams, ...

  13. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...How are you currently exchanging information with your team? If you need to exchange information regarding...which they are sure that there will be no sharp changes in costs once they establish the system within ...consolidated to one place, easily retrievable, searchable and traceable. However, switching from paper ...

  14. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ... of reasons to consider using genome editing to change the genetic code of plants, including the development... system in most organisms, some plant-specific changes to CRISPR components are necessary to enable genome...try your hand at CRISPR. References Belhaj K, Chaparro-Garcia A, Kamoun S, Nekrasov V (2013) Plant genome.../mmbr.67.1.16-37.2003 Khatodia S, Bhatotia K, Passricha N, Khurana SMP, Tuteja N (2016) The CRISPR/Cas...

  15. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ... do you need [for your lab start up]?” A hiring chair or dean will often ask a faculty candidate. Sweaty...focusing on long term investments. For example, purchasing “used” equipment can save you money in the short...their tangible resources. For example, if a dean/chair asks “how much do you need to start your lab”. You..., working with this question is second nature. Channel your inner curiosity and use your research training...

  16. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...giving greater concentration of cut DNA without changing the size. As a result, a restriction digest of...reducing multimerization and define recA-independent mechanisms. Avoid overgrowing the bacterial culture Another...PMCID: PMC3060453. Bazaral M, Helinski DR. Characterization of multiple circular DNA forms of colicinogenic...Adv. 2009 Jul-Aug;27(4):353-70. doi: 10.1016/j.biotechadv.2009.02.003. Epub 2009 Feb 20. PMID: 19233255...

  17. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ... loci of integration [8].   Figure 1: Mechanism of the piggyBac™  transposase/transposon gene ...CRISPR/Cas9, TALENs, and ZFNs). However, several challenges still remain, such as making gene edits completely...nuclease technology with the piggyBac™ system, these challenges can be considerably reduced. The piggyBac™ transposon...researchers to avoid making unnecessary base pair changes as would be the case with ssODNs. One minor drawback...

  18. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ... genome editing system you use, there remains a chance that the observed phenotype was caused by an off-target...25566793. PubMed Central PMCID: PMC4349094. 3. Bell, Charles C., et al. "A high-throughput screening strategy...: 237-243. PubMed PMID: 25664545. 10. Gabriel, Richard, et al. "An unbiased genome-wide analysis of zinc-finger...Therapy (2015). PubMed PMID: 26502778. 13. Frock, Richard L., et al. "Genome-wide detection of DNA double-stranded...

  19. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...in a screen, to ensure that each gRNA has a good chance to contribute to the screen. In the end, this results...that code for surface proteins. One of the first challenges with a custom screen approach is to identify ...Wu (4), Yusa (5), Wang/Lander/Sabatini (6), and Chari/Mali/Church (7). These reference libraries contain... 28162770. PubMed Central PMCID: PMC5445660. 7. Chari, Raj, et al. "Unraveling CRISPR-Cas9 genome engineering...

  20. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ... called SNAPf. eDHFR: The Virginia Cornish and Michael Sheetz labs demonstrated in 2005 that proteins ...and see the results right away. Or perform pulse-chase experiments by sequentially adding different ligands...All this flexibility comes at a price, though. Purchasing lots and lots of organic dyes or specialized ...Johnsson, K. (2021). Kinetic and Structural Characterization of the Self-Labeling Protein Tags HaloTag7...
Showing: 361 - 380 of 811 results