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We narrowed to 763 results for: ire;

Showing: 361 - 380 of 763 results

  1. Michael Koeris' Journey from Grad Student to Entrepreneur: The Story of Sample 6

    Type
    Blog Post
    Published
    Jan. 21, 2016, 4:52 p.m.
    ... taking up a position on the Addgene Board of Directors. We're excited to watch both Addgene and Sample...How to Be A Good Manager from Addgene Executive Director Joanne Kamens Resources on the Addgene Website... Check Out the Other Members of our Board of Directors Become a Part of the Addgene Community ...

  2. Science Communication for Everyone

    Type
    Blog Post
    Published
    April 16, 2019, 1:05 p.m.
    ...every science field as we expand and encourage more direct conversations between scientists and non-scientists...process of starting a forum where non-scientists can directly approach scientists and doctors with their questions.... Use hashtags effectively. This would help you direct your content to the right audience. Avoid using...

  3. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ...meteoric rise of CRISPR technology, the ability to direct enzymes – from nucleases to transcription factors...RNA. As a result, targeting the RNA of interest requires that the RNA be tagged. “This tool makes it possible...She also notes that for reasons that are not yet entirely clear some PUF proteins lose their solubility....

  4. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...) that can be repaired by either non-homologous end joining (NHEJ) or homology directed repair (HDR). ...CIB1 fusion binds to the target DNA sequence as directed by the guide RNA (gRNA), while the CRY2PHR-activator...employed in other split systems where light is required to reconstitute enzymatic activity. Photoactivatable...

  5. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    Published
    Jan. 8, 2015, 2:34 p.m.
    ...information to make improvements. Members connect directly with one another – not just with the team leader...'t work to get people sharing.   Frequently ask direct questions for specific feedback in non-public settings...Scientists. Subscribe to the Addgene Blog to follow the entire series as it is published. For more reading try...

  6. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    Published
    May 8, 2014, 3:07 p.m.
    ...work, determination, passion, and patience are required to build and lead a productive and successful ...sharing meals, going on trips, and going to New Hampshire in the summer for the annual lab retreat. How ...no play Although hard work and perseverance are required to achieve success in the lab, it is important...

  7. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    Published
    June 8, 2023, 1:15 p.m.
    ...to dive into conversations with other users. The entire dataset can be downloaded from Zenodo, including... the Reagent Resources table or by downloading directly from the Zenodo dataset.     Figure 2... and answer questions. Andrea and Ziv were inspired by the open-software community when designing ...

  8. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...transgene (Lin et al., 2018). However, this system requires precise titration of the AAV. So far, tools to...to overcome this challenge in Drosophila require heat-shocking the system or using chemical inducers of... that will be labeled in a given population is directly correlated to the length of the truncated attP...

  9. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ... in the body, like the gastrointestinal tract. Require Administration of Substrate: Some luciferase reporters... such as Renilla luciferase (RLuc) or NanoLuc, require injection of substrate to produce bioluminescence...arg2 vesicles is the amount of soundwave pressure required to collapse them: arg2 vesicles collapse at a ...

  10. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ... Matchmaker Day 2: Homology directed repair: CRISPR 101: Homology Directed Repair and 3 Tips to Improve...101: Multiplex Expression of gRNAs and The PAM Requirement and Expanding CRISPR Beyond SpCas9 Day 8: Repair...

  12. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...    Novel fluorescent enzyme biosensors provide direct, specific and sensitive detection of the plant ...strigolactones (SLs) are costly, non quantitative or require heavy optimization. Claudia Vickers’s lab has designed...than on the fluorescence intensity, it provides a direct and quantitative detection of SLs. The authors ...

  13. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ... the main source of the body’s DA. Neurons often fire at a certain frequency, and this frequency can indicate...frequency of its release) are well understood, the direct effect of DA binding to a target neuron is more...the region of the brain where DA quantitation is desired (Figure 1). The probe perfuses liquid, which causes...

  14. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type
    Blog Post
    Published
    June 11, 2019, 1:16 p.m.
    ...are under a Creative Commons 3.0 license, which requires users to give appropriate credit, provide a link...indicate if changes were made to the images. This requirement means the images are probably better suited for... image search is a great tool for searching the entire internet for scientific graphics. The tools settings...

  15. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ...Running4Rare are paired with patient partners so that they can help tell their stories. I was paired with a six-year-old...where my wife Lianna has made her career as Senior Director of Biology, is helping us fight against FOP in...

  17. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...cells by any means, but are unique in that they require delivery to be viral-mediated. Transformations ...retrovirus, lentivirus, adenovirus, or AAV. Then, your desired DNA to be integrated can be cloned into the vector...weeks, depending on the virus, cell type, and/or desired event.   Figure 1: Transduction to expression...

  18. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    Published
    June 2, 2020, 1:15 p.m.
    ...rate discovery rate, conventional CRISPR screens require a low multiplicity of infection (MOI) during cell... tools for doing this were labor-intensive and required using manipulations such as injection of precise...drives gene expression. Because calcineurin is required for virulence in pathogenic fungi, it is an attractive...

  19. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    Published
    March 5, 2020, 2:15 p.m.
    ...using AAV on our blog. Image: The final and required step requires submission of an experimental image result...expression of AAV cargo? Did you use GCaMP expression? Direct Fluorescence? Immunohistochemistry? Did you use...

  20. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ...in the last few months. You can also browse our entire AAV inventory. Our new AAVs include: Optogenetics... See our Serotype Testing AAV inventory for the entire collection! Plasmid Serotype Name 51502 AAV5... sensors provide the spatiotemporal resolution required for precise in vivo measurements of dopamine (...
Showing: 361 - 380 of 763 results