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We narrowed to 632 results for: ncl

Showing: 361 - 380 of 632 results
  1. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...appreciated. PhosphoSitePlus, one of the most inclusive databases of post-translational modifications,...pSer-incorporated proteins on a single plasmid. This includes 4 copies of a tRNA that specifically recognizes...
  2. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ... search suggests there are hundreds. This only includes general lab strains designed for subcloning or...or protein expression. If you were to include customized strains, the number is probably in the thousands...
  3. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ... variants that are less sensitive to ethanol, including the “middle” ADH1 promoter pADH1m, which maintains...3 in a single modular vector for ease of use! Conclusion This post contains only a brief overview of some...
  4. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...plasmid sequencing services to verify the plasmids, including long-read sequencing techniques, such as Oxford... presence of a multimer in your sample remains unclear after visualizing it on a gel, one of the other...
  5. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...stop and plan it out. Some questions to answer include: What is the goal of the experiment? What are...Howard Hughes Medical Institute. His interests include the quality control of secretory proteins in the...
  6. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ... infect both dividing and non-dividing cells, including stem cells. These properties make lentiviral vectors..., a separate genus of the Retroviridae family including human immunodeficiency virus type-1 (HIV-1), can...
  7. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...Cre recombinase contained within the knock-in. Including Cre-mediated marker excision, it takes approximately...that recipients for the 2015 Breakthrough Prize included Jennifer Doudna and Emmanuelle Charpentier for...
  8. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...in yeast, plant, insect, and mammalian cells, including human cells. piggyBac is biased to integrate at...all been used to generate transgenic animals, including zebrafish, mice, rats, and rabbits (Sandoval-Villegas...
  9. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ... still valuable for anyone in the lab though, including undergrads. I found a lot of career resources ...in microbes until the end of my second year! Conclusion and other pieces of advice Give yourself permission...
  10. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...with minimal cross-reactivity. Other strategies include using engineered ligases to attach a ligand to ...study your gene of interest with these tools. Conclusion Researchers and vendors continue to develop new...
  11. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...CRISPR will likely depend on a variety of factors, including your goal, experience, hypothesis, and even the..., D., Shah, S. A., Siksnys, V., Terns, M. P., Venclovas, Č., White, M. F., Yakunin, A. F., . . . Koonin...
  12. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...information related to your plasmid. It is optional to include the total size of the insert and the GenBank ID...submission, please email us at [email protected]. Include your deposit number and which data needs to be ...
  13. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ... Paramyxoviridae family of viruses, which also includes measles and mumps. Sendai transduces a wide range...the field of iPSCs Find gene-specific plasmids (including reporters and RNAi) for:  NANOG  OCT4 SOX2 MYC...
  14. Addgene Launches Partnership with IPI

    Type
    Blog Post
    Published
    June 13, 2023, 1:15 p.m.
    ...educational resources on our website and blog, including our antibody guide, antibody protocols, and Antibodies...
  15. Viral Vectors 101 eBook 2nd Edition

    Type
    Blog Post
    Published
    July 11, 2024, 1:15 p.m.
    ...AAVs in your experiment.      Our second edition includes new and updated content in a clean and easy-to-navigate...
  16. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ...content. Access the CRISPR Guide here! Highlights include a much-anticipated prime editing section and additions...
Showing: 361 - 380 of 632 results