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Addgene

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We narrowed to 394 results for: organization Addgene

Showing: 361 - 380 of 394 results

  1. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...PMCID: PMC4831023. Additional Resources on the Addgene Blog Learn More About Fluorescent Protein Aggregation...there were a variety of ways to monitor cell, organelle, and protein localization. For instance, you might...microscope, fractionate samples to isolate particular organelles and their contents, or perform in situ hybridization...Choosing Your Fluorescent Protein Resources at Addgene.org Find Fluorescent Protein Timers Browse All Fluorescent...

  2. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    Published
    July 29, 2014, 6:16 p.m.
    ...researchers as we could, and Addgene is a wonderful vehicle for us to do that.” At Addgene, we are equally excited...is available in an easily searchable format on Addgene’s Michael Davidson Fluorescent Protein Collection...collection with over 300 backbones alone,” said Addgene’s Lianna Swanson, who has been working with members...the scientific community," says Joanne Kamens, Addgene's Executive Director, "and to promote the great ... Kendall Morgan...

  3. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    Published
    April 22, 2015, 2:06 p.m.
    ...things are easy with CRISPR. Addgene: You’ve made many deposits to Addgene over the years. Can you describe...biology, the tools he’s making available through Addgene, and where all of it is likely to lead us in the...the future. Building circuits from CRISPRs Addgene: What can CRISPR do for synthetic biology? Voigt...conceivably be as large as natural regulatory networks. Addgene: What kinds of things could we do as genetic circuits.... It requires more complex synthetic networks. Addgene: What hurdles did you have to overcome and what...regarding the size of the networks you could build. Addgene: Is this what you are working on now? Voigt: We...taking on very large genetic engineering projects. Addgene: How easy is it to do this now? Voigt: One of the...

  4. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...versions of 15 different disease-associated genes. Addgene spoke to Musunuru about how the new kit works, ...and how it compares to his CRISPR/Cas9 system. Addgene: For people unfamiliar with this new TALEN kit,...either knock out a gene or knock in a mutation. Addgene: How do you use this kit to target a particular...cells. (See the complete protocol at StemBook). Addgene: How are you now using this tool? Musunuru: We ...one needs to do disease modeling in stem cells. Addgene: Tell me about the history. How had investigators...genetically modified cells in less than a month's time. Addgene: What are the implications of this kit in terms...becomes more of an apples-to-oranges comparison. Addgene: Can you compare the TALEN kit to CRISPR/Cas9? ...

  5. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    Published
    Aug. 28, 2018, 12:04 p.m.
    ...way to visualize them. You can also “hear” them. Addgene depositor Dr. Mikhail Shapiro from Caltech presented...PubMed Central PMCID: PMC5920530.  Resources on the Addgene Blog Read Our Post on CRISPR Methods for Bacterial...bacteria Deposit Your Microbiology Plasmids with Addgene ...Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts." Nature 553.7686 (2018)...Science Communication using Twitter  Resources on Addgene.org Check out Our Microbiology Resource Page CRISPR...

  6. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...those rare diseases will receive plasmids from Addgene worth a total of $5000.  Lowe Syndrome One of those...the award from the Rare Genomics Institute and Addgene will make a substantial difference in pushing the...iPS) technology and CRISPR-Cas9 constructs from Addgene to gene-edit mutations into XP genes and other ...analyses.  “We believe that with this award from Addgene, we’ll be able to make big progress in elucidating... Kendall Morgan...

  7. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type
    Blog Post
    Published
    Sept. 3, 2019, 1:15 p.m.
    ... al., 2019). Find the mAID tagging toolkit at Addgene Yesbolatova et al., Methods, 2019. PubMed PMID:...difficult. Find SpCas9 and Target-AID-NG plasmids at Addgene! Nishimasu H et al., Science, 2018. PubMed PMID...research and innovation. Find MerMAIDs plasmids at Addgene Opperman J et al., Nature Communications, 2019....plasmids at Addgene Kim et al., Nat Methods. 2019, PubMed PMID: 31235883. ...new family of seven ChRs derived from marine microorganisms based on metagenomic data. MerMAIDs (Metagenomically... Various Addgenies...

  8. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...about the ClonTracr Library Resources on Addgene.org Addgene's most popular lentiviral plasmids Overview...lentiviruses can infect dividing and non-dividing cells.  Addgene has an extensive collection of lentiviral plasmids...lentiviral questions. This useful resource from Addgene Depositor (and lentiviral expert) Didier Trono'...gritty and answer the questions those of us at Addgene get asked most often: Q1: Are retroviruses and ...and 3rd-generation transfer vectors apart? A3: Addgene defines 2nd- or 3rd-generation transfer vectors... competent? What is SIN? A6: Most (if not all) Addgene transfer plasmids are replication-deficient meaning...Trono Lab: Lentivectors Toolbox Resources on the Addgene Blog: Download our eBook: Plasmids 101 Learn about...

  9. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ... for the generation of human disease models." Addgene Outreach Scientist and fellow blogger Caroline ...CRISPR Plasmids? CRISPR-Cas9 Plasmids available at Addgene CRISPR-Cas9 References, Protocols, and more References... Kendall Morgan...

  10. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...article can be obtained through Addgene: Rapamycin-inducible Cas9 sets (Addgene plasmids 62883 &62884; 62885...At Addgene we're continually impressed with the amazing plasmid technologies developed by our community... Rapamycin-inducible dCas9-VP64 activator set (Addgene plasmids 62887 & 62888) Zetsche et al., Nat ...brilliant SunTag system plasmids are available through Addgene and are already very popular. Use these plasmids...pathways (Figure 2). They are all available now at Addgene. 1 Lungu et al., Chem Biol. 2012 Apr 20;19(4..., and other plasmids from this article through Addgene. Nihongaki et al., Chemistry and Biology. 2015...human genes. The plasmids are all available at Addgene ready to light up the genome of your favorite cells...

  11. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ...will make those new plasmids available through Addgene, and they hope others will do the same. “This isn...Resources Addgene's Synthetic Biology Overview More Synbio Blog Posts ... Kendall Morgan...

  12. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ...vectors to generate a kit which is now available at Addgene. “We called it cloning by mail,” Wood says, and...PMCID: PMC4353587. Additional Resources on the Addgene Blog Learn How the Clon Tracer Library Has Been...growth, survival, and differentiation.” https://www.addgene.org/kits/sabatini-wood-cancer-pathway-orfs/ Finding...Technologies from 2015 Additional Resources on Addgene.org Find Other Kits for Your Research Find Pooled... Kendall Morgan...

  13. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...Communication Additional Resources on Addgene.org Find Job Openings at Addgene ...started:  Subscribe to Career Blog Posts from Addgene 1) Twitter – Get your voice out there Here’s one...today, Soapbox Science (hosted by Nature), and Addgene are open to guest contributions. If you decide ...BananarootsBlog.   Additional Resources on the Addgene Blog Check out Other Science Communication Posts...work or volunteer for a science communication organisation like Building with Biology. Local environmental...

  15. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ... wide range of fields from Addgene. What you might not know is that Addgene distributes curated collections...Sciences 108.38 (2011): 15870-15875. See Kendall Morgan’s blog post on the PUF Assembly Kit for some insights...to deposit a kit, please contact us at depost@addgene.org. ...

  16. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...protein-by-protein basis. However, since many of Addgene’s SunTag plasmids have coveted “blue flames,” it...PMCID: PMC4252608. Additional Resources on the Addgene Blog Sign Up to Receive the Fluorescent Protein...obtained bright images of mitochondria without organelle toxicity. First generation (v1) SunTag is expressed...about Small Molecule Fluorphores Resources on Addgene.org Find Fluorescent Protein Empy Backbones Visit...

  17. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...sequencing, and optical pooled screens in human cells. Addgene offers vectors for CROPseq-multi using selection...recombinant antibodies were recently added to the Addgene repository of tools for SAR-CoV-2 research. Two...critical component for viral genome packaging. Addgene recommends both for use in western blots (Figure...as a loading control (right panel). Image from Addgene. These antibodies are recombinant versions ... out the new and noteworthy tools featured in Addgene’s Neurodegeneration Research Collection. This page...vitro and in vivo, including in human retinal organoids and live mice. Importantly, REVeRT avoids the ...

  18. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...vectors Learn about Addgene AAV Quality Control Process Resources on Addgene.org Find ready-to-use AAV...PubMed PMID: 25665714. Additional Resources on the Addgene Blog Check out our Viral Vectors Featured Topic... AAV in Addgene's Viral Service Check out our AAV Production Protocol Check out our AAV Purification Protocol...investigated including lentiviruses, adenovirus, and inorganic delivery tools such as nanoparticles. These technologies...
Showing: 361 - 380 of 394 results