...PMCID: PMC4831023. Additional Resources on the Addgene Blog Learn More About Fluorescent Protein Aggregation...there were a variety of ways to monitor cell, organelle, and protein localization. For instance, you might...microscope, fractionate samples to isolate particular organelles and their contents, or perform in situ hybridization...Choosing Your Fluorescent Protein Resources at Addgene.org Find Fluorescent Protein Timers Browse All Fluorescent...

...way to visualize them. You can also “hear” them. Addgene depositor Dr. Mikhail Shapiro from Caltech presented...PubMed Central PMCID: PMC5920530.  Resources on the Addgene Blog Read Our Post on CRISPR Methods for Bacterial...bacteria Deposit Your Microbiology Plasmids with Addgene ...Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts." Nature 553.7686 (2018)...Science Communication using Twitter  Resources on Addgene.org Check out Our Microbiology Resource Page CRISPR...

...those rare diseases will receive plasmids from Addgene worth a total of $5000.  Lowe Syndrome One of those...the award from the Rare Genomics Institute and Addgene will make a substantial difference in pushing the...iPS) technology and CRISPR-Cas9 constructs from Addgene to gene-edit mutations into XP genes and other ...analyses.  “We believe that with this award from Addgene, we’ll be able to make big progress in elucidating... Kendall Morgan...

...about the ClonTracr Library Resources on Addgene.org Addgene's most popular lentiviral plasmids Overview...lentiviruses can infect dividing and non-dividing cells.  Addgene has an extensive collection of lentiviral plasmids...lentiviral questions. This useful resource from Addgene Depositor (and lentiviral expert) Didier Trono'...gritty and answer the questions those of us at Addgene get asked most often: Q1: Are retroviruses and ...and 3rd-generation transfer vectors apart? A3: Addgene defines 2nd- or 3rd-generation transfer vectors... competent? What is SIN? A6: Most (if not all) Addgene transfer plasmids are replication-deficient meaning...Trono Lab: Lentivectors Toolbox Resources on the Addgene Blog: Download our eBook: Plasmids 101 Learn about...

... al., 2019). Find the mAID tagging toolkit at Addgene Yesbolatova et al., Methods, 2019. PubMed PMID:...difficult. Find SpCas9 and Target-AID-NG plasmids at Addgene! Nishimasu H et al., Science, 2018. PubMed PMID...research and innovation. Find MerMAIDs plasmids at Addgene Opperman J et al., Nature Communications, 2019....plasmids at Addgene Kim et al., Nat Methods. 2019, PubMed PMID: 31235883. ...new family of seven ChRs derived from marine microorganisms based on metagenomic data. MerMAIDs (Metagenomically... Various Addgenies...

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...article can be obtained through Addgene: Rapamycin-inducible Cas9 sets (Addgene plasmids 62883 &62884; 62885...At Addgene we're continually impressed with the amazing plasmid technologies developed by our community... Rapamycin-inducible dCas9-VP64 activator set (Addgene plasmids 62887 & 62888) Zetsche et al., Nat ...brilliant SunTag system plasmids are available through Addgene and are already very popular. Use these plasmids...pathways (Figure 2). They are all available now at Addgene. 1 Lungu et al., Chem Biol. 2012 Apr 20;19(4..., and other plasmids from this article through Addgene. Nihongaki et al., Chemistry and Biology. 2015...human genes. The plasmids are all available at Addgene ready to light up the genome of your favorite cells...

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...vectors to generate a kit which is now available at Addgene. “We called it cloning by mail,” Wood says, and...PMCID: PMC4353587. Additional Resources on the Addgene Blog Learn How the Clon Tracer Library Has Been...growth, survival, and differentiation.” https://www.addgene.org/kits/sabatini-wood-cancer-pathway-orfs/ Finding...Technologies from 2015 Additional Resources on Addgene.org Find Other Kits for Your Research Find Pooled... Kendall Morgan...

...Communication Additional Resources on Addgene.org Find Job Openings at Addgene ...started:  Subscribe to Career Blog Posts from Addgene 1) Twitter – Get your voice out there Here’s one...today, Soapbox Science (hosted by Nature), and Addgene are open to guest contributions. If you decide ...BananarootsBlog.   Additional Resources on the Addgene Blog Check out Other Science Communication Posts...work or volunteer for a science communication organisation like Building with Biology. Local environmental...

... wide range of fields from Addgene. What you might not know is that Addgene distributes curated collections...Sciences 108.38 (2011): 15870-15875. See Kendall Morgan’s blog post on the PUF Assembly Kit for some insights...to deposit a kit, please contact us at depost@addgene.org. ...

...appreciate the humor in science and in the lab? Addgene is after all a place that encourages people to ...  Kendall Morgan...

...protein-by-protein basis. However, since many of Addgene’s SunTag plasmids have coveted “blue flames,” it...PMCID: PMC4252608. Additional Resources on the Addgene Blog Sign Up to Receive the Fluorescent Protein...obtained bright images of mitochondria without organelle toxicity. First generation (v1) SunTag is expressed...about Small Molecule Fluorphores Resources on Addgene.org Find Fluorescent Protein Empy Backbones Visit...

...sequencing, and optical pooled screens in human cells. Addgene offers vectors for CROPseq-multi using selection...recombinant antibodies were recently added to the Addgene repository of tools for SAR-CoV-2 research. Two...critical component for viral genome packaging. Addgene recommends both for use in western blots (Figure...as a loading control (right panel). Image from Addgene. These antibodies are recombinant versions ... out the new and noteworthy tools featured in Addgene’s Neurodegeneration Research Collection. This page...vitro and in vivo, including in human retinal organoids and live mice. Importantly, REVeRT avoids the ...

...vectors Learn about Addgene AAV Quality Control Process Resources on Addgene.org Find ready-to-use AAV...PubMed PMID: 25665714. Additional Resources on the Addgene Blog Check out our Viral Vectors Featured Topic... AAV in Addgene's Viral Service Check out our AAV Production Protocol Check out our AAV Purification Protocol...investigated including lentiviruses, adenovirus, and inorganic delivery tools such as nanoparticles. These technologies...

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...open access journals.   Check Out Addgene Videos Over at the Addgene Youtube Channel   After watching ...Communication 2.2 (2014): 2. Additional Resources on the Addgene Blog 6 Tools & Tips: Online Social Networking ...study processes for diagnosing and resolving organizational performance and often have to be able to quickly...Sharing in Research Publications Resources at Addgene.org Check out our Protocol Pages for Video Protocols...