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We narrowed to 392 results for: plasmid dna

Showing: 361 - 380 of 392 results

  2. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ... M., Urbaniak, S. et al. Review: immunoassays in DNA damage and instability detection. Cell. Mol. Life...Resources on Addgene.org Browse Addgene’s Antibodies plasmid collection ...

  3. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...essential-but-unlauded role in the identification of DNA’s double helix. Perhaps to the dismay of middle-aged...and cheap distribution of the newest CRISPR-Cas plasmids, has been a key driver of the technology.” As ...fields, and seeing names like Feng Zhang, Jennifer Doudna, Karl Deisseroth, and Ed Boyden crop up in this...

  4. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ...currently wrapping up my co-op with Addgene as a DNA Sequencing Analyst intern. As a member of the Quality...Quality Control team, I help make sure the plasmids that go out our doors are correct. Now that I am heading...

  6. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...use the chromatin IP (ChIP) technique to identify DNA binding proteins involved in histone modification...antibody posts  Resources on Addgene.org: Find plasmids encoding recombinant antibodies at Addgene ...

  7. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...expensive) synthetic fluorophores. Add a dash of DNA with an electrical jolt and cells become perfectly... Generate Fluorescent Protein Fusions Request Plasmids from the Michael Davidson Collection Find Fluorescent...

  8. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    Published
    Sept. 13, 2018, 1:55 p.m.
    ... non-targeting guides. Toxicity is likely due to DNA damage and its subsequent repair. Additionally, the...on Addgene.org Check out this guide to pooled plasmid libraries Find all of the CRISPR Pooled Libraries...fail to account for the effects of Cas9-induced dsDNA breaks. Are off-target cutting patterns in genome-wide...function, i.e. sites that lack open chromatin marks, DNase hypersensitivity, or are in an enhancer, transcription...control for the effects of guide expression and dsDNA breaks. Figure 2: Non-targeting vs. safe-targeting...for the inherent effects, or background noise, of dsDNA breaks caused by CRISPR. See supplemental figure...

  9. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...freezer, you’ll probably find lots of different plasmids containing the same protein of interest tagged...developed a self-labeling fusion tag based on the human DNA repair protein AGT that gets covalently linked to...Addgene’s collection, so you may be able to get a plasmid ready-to-use without any cloning at all! As mentioned...

  10. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ... the mammalian brain (Wickersham et al., 2007; plasmids at Addgene). AAV vectors: A platform for delivering...neuronal cells. The AAV genome is a single-stranded DNA molecule and AAV vectors are replication deficient...involved in learning and memory (Kohara et al., 2014; plasmids at Addgene). The Gradinaru Lab at the California... throughout the brain based on its unique hue (plasmids at Addgene). A large-scale example of neuronal...than any other AAV serotype (Tervo et al., 2016; plasmid at Addgene). Its efficiency is comparable to conventional...Addgene.org Find ready-to-use AAV Preps Find AAV plasmids Browse all viral vectors ...

  11. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...relatively new technology that takes advantage of DNA sequencing to turn mAb clones into expression constructs...Antibodies 101: ValidationSelecting the Right AntibodyPlasmid-based Recombinant Antibodies    ...

  12. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...in analysis Plasmid-based proteins: plasmid sequence not accurate Check plasmid sequence for frameshifts...specific compartment. If your proteins are nuclear- or DNA-binding proteins, you may need to sonicate your lysates...

  13. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...of the new plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. ... 2022.   Find CRISPR Csm plasmids here! Colognori D, Trinidad M, Doudna JA. Precise transcript targeting... missed something smokin'? You can pitch a Hot Plasmids section (antibodies and viral preps welcome!) ...inducible expression.   Find heat-inducible lentiviral plasmids here!   Wu Y, et. al. Control of the activity ...2: Addgene’s western blot for mEGFP-HRas (from Plasmid 18662). See the Applications section on the Anti-GFP... Campbell et al, Nature Methods   Find YFP plasmids here!   Campbell, B.C., et al. Chemically stable...novel RNA targeting system by: Susanna Stroik The Doudna lab’s recent work characterized a new tool for ...

  14. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...packaging plasmids. The cells need three different plasmids to produce AAV: 1) the RepCap plasmid which provides...going to lie, AAV transfer plasmids are kind of a pain to work with. These plasmids contain ITRs which are... AAV transfer plasmids. It’s easy to contaminate a workspace with plasmid, not that I would know about...prep. AAV samples are first DNaseI digested to remove any residual AAV plasmid that was carried over from...sample for the DNAseI digest. Use good sterile technique when handling your AAV plasmid standard and AAV...AAV’s protein shell, or capsid; 2) the pHelper plasmid which expresses adenovirus genes which help mediate...mediate AAV viral replication; and 3) the transfer plasmid which encodes a transgene of interest that’s packaged...

  15. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    Published
    May 11, 2017, 2:30 p.m.
    ...We've developed a new plasmid toolkit, the Broad Institute Target Accelerator Plasmid Collection, that we...phenotypes. Broad Institute Target Accelerator Plasmid Collections To overcome this first challenge, we...developed the Broad Institute Target Accelerator Plasmid Collections, now widely available to the scientific...in our recent publications, we used the cancer plasmid collections listed above to demonstrate how gene... hope that by releasing these new and exciting plasmid collections (together with the data generated from...patients. We hope you find these new cancer variant plasmid collections useful for your studies. Many thanks... Addgene.org Fine More Kits at Addgene Browse Plasmids from the Broad Institute ...

  16. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...you find what you need.   More Plasmid Resources from Addgene: Plasmids 101 blog series Addgene's Molecular...need. You will also have to make sure that your plasmid has been incorporated into the host organism, usually...open reading frame (ORF) of interest –  try pRS plasmid shuttle vectors. You may prefer the more traditional...details and guidance for choosing your perfect plasmid backbone. Or just ask –  leave a message in the...Molecular Cloning Guide & Other Plasmid Protocols List of common epitope tags ...mammalian expression vectors, c-Flag pcDNA3, comes from the pcDNA series. It has the CMV promoter for ...protein will allow you to visualize YGOI in vivo (try pcDNA3-EGFP). If you can get away with generating transiently...

  17. Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes

    Type
    Blog Post
    Published
    Feb. 6, 2025, 2:15 p.m.
    ...information on shipping plasmids to your country. Curious about how rabies plasmids are used in the lab? ...If you're an international researcher who uses plasmids containing genes from rabies and vesicular stomatitis...may have noticed it’s been easier to order these plasmids. This is because in 2024 certain export control...controls prescribed by the Australia Group, for these plasmids for the majority of the countries we ship to, ...lasting basis.   Table 1: Popular Rabies and VSV-G Plasmids Name Purpose Depositing Lab pCMV-VSV-G ... Weinberg pMD2.G VSV-G envelope expressing plasmid Didier Trono pVSV-G Expresses VSV-G envelope...control exceptions for rabies and VSV-G genes, plasmids ship out approximately three weeks faster, as ...

  18. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...four different plasmids into the producer cells (two packaging plasmids, an envelope plasmid, and the lentiviral...generation systems consist of an envelope plasmid and a packaging plasmid encoding HIV proteins gag, pol, rev...generation systems require an envelope plasmid, and two packaging plasmids, one encoding gag and pol and a second...transfer plasmids replication competent? What is SIN? A6: Most (if not all) Addgene transfer plasmids are ... 30% of the transfer plasmid. Many transfer vectors are SIN. Packaging plasmid(s) contain the minimal...Addgene's most popular lentiviral plasmids Overview of Lentiviral Packaging Plasmids Lentiviral Protocols and ...collection of lentiviral plasmids created for a variety of applications including cDNA expression, shRNA-mediated...

  19. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...received over 13,000 plasmids requests for COVID-19 related plasmids. These plasmids have reached labs in...small collection of plasmids for coronavirus research, including a handful of plasmids that have been in...some highlights of the plasmids shared over the last year and how these plasmids have been used to accelerate...circulating and plasmids containing these mutations have been deposited at Addgene. Plasmids deposited from... labs also deposited plasmids for producing COVID-19 diagnostic enzymes. Plasmids in this toolkit include...These plasmids contain both untagged and tagged versions of SARS-CoV-2 ORFs and include plasmids that ...scientists through plasmid sharing and more. As you’ve read above, some of these plasmids have been in the...

  20. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...comparison of an shRNA plasmid sequence and an miRNA plasmid sequence. (A) Plasmid sequence from pLVUHshp53...between the red boxes. (B) Plasmid sequence from pcDNA3-miR21 (Addgene plasmid #21114). The miRNA sequence....   Expressing shRNAs using plasmids The design of shRNA plasmids requires three important steps: ...shRNA plasmids are being designed using RNA polymerase II promoters. The design of these plasmids is meant...with Cas13a (C2c2) Plasmids 101: The Promoter Region – Let's Go! Browse All Plasmids 101 Posts Additional... occur via transfection or viral delivery of a plasmid. RNAi is an umbrella term for a number of different...Following delivery into cells using viral vectors or plasmids, shRNAs hijack the endogenous miRNA machinery ...
Showing: 361 - 380 of 392 results