We narrowed to 799 results for: por

...expressed, the gRNAs cannot be released, adding temporal and/or spatial control to the system. pSQT1313...plasmids, which contain various promoters and reporters, and subsequently inserted into a Cas9-containing...-in-one CRISPR/Cas9 vector system.” Scientific Reports 4 (2014): 5400. PubMed PMID: 24954249. PubMed Central... Find plasmids from this paper at Addgene. 10. Port, Fillip, et al. “Optimized CRISPR/Cas tools for ...

...story of Rosalind Franklin and her incredibly important role in solving the structure of DNA. 2. The...popular videos. Describes fossil rocks and their importance in understanding biological history. 4. Please...   5. Plasmid Rap Plasmid rap video made with support from Addgene.      Check out more science education...

...Realizing that Addgene would be successful and the importance of lentiviral vectors in ensuring that success...international plasmid distribution!) Chapter 8 - Opportunities to create repositories with other materials ... Addgene's Future, the research team, and the importance of sharing information/knowledge Outro Many ...

...grant proposals or to demonstrate impact in annual reports. While the raw data is, of course, interesting ...features of dashboards are (i) easy access to important numbers and trends, (ii) user interaction with...dashboard at-will with new data. These features are incorporated in the current dashboard. The dashboard is coded...

...nonprofit founded in 2007, strives to expand the opportunities and scientific resources available to researchers...facilitating connections to mentorship and networking opportunities, publishing assistance, and more to Instrumental...Seeding Labs, Addgene also feels it’s incredibly important for scientific breakthroughs, reagents, and know-how...

... NIH-funded researchers and utilizes grants to support our own initiatives, Addgene is deeply concerned...with partners across the research ecosystem to support the essential work of our scientific community ...funding changes are impacting your work and what support would be most valuable from Addgene during this...

...: Topic 0:00 - 0:50 : Intro 0:51 - 3:08 : The importance of developing a strong graduate student community...you changed throughout graduate school? - The importance of criticism and feedback 15:38 - 17:06 : Post... - 18:29 : How did you hear about post grad opportunities and when did you start thinking about them? ...

...purifying a plasmid from a bacterial culture, it is important to consider your experiment.  It will dictate ...To allow efficient and flawless cloning it is important that the preparation is pure from other nucleic...increase transfection success.  In conclusion, it is important for you to establish in advance how you want to...

...smooth and simple as possible. We are the Customer Support Specialists here at Addgene. If you’ve called our...order? I tried searching my PO in the track order portal and nothing shows up. Where is my order? All of... store your plasmids or viral items, technical support, and more. ...

...stress and depression, and now researchers have reported a mechanism based on studies of transgenic mice... there is plenty more to explore. “It will be important to identify the key proteins and metabolites that...stress-induced depression. Agudelo LZ, Femenía T, Orhan F, Porsmyr-Palmertz M, Goiny M, Martinez-Redondo V, Correia...

...creating their own opportunities for career development and networking. Aside from the importance of the topics...transferable skills to enable pursuit of these diverse opportunities after training. One of the best recent discussions...

...mutations, both in mice and in human cells. But that important advance was quickly followed by even splashier...All this, and scientists are still working out important details in their understanding of how CRISPR/Cas9s...at the Broad Institute and MIT published another report showing Cas9's structural details. "Understanding...

...We continue to expand our educational content supporting the tools in our collection, such as blogs describing...viral vector serotypes, or hundreds of Data Hub reports detailing antibody experimental results. We also...exactly what you need, faster. We’re also building a portal to easily share sequences and characterization ...

...I’m sitting in the San Francisco International Airport listening to the Lion King soundtrack and writing.... I love running and this event gives me the opportunity to dress up as Mr. Incredible, an added bonus...would like to give my thanks to all the Addgene supporters who spoke with me and deposited plasmids on this...

...focus on an aspect of communication especially important for a manager – getting feedback from the team... This can take practice and training. This is important because it creates a culture of engagement in ...  Seeking feedback frequently One of the most important paths of information flow is the one that brings...

...over $100 million in annual R&D expenditures to support increased public access to the results of federally-funded...on the global science enterprise lending their support to open scientific research data around the world...online survey of scientists showing overwhelming support for open access to peer-reviewed journal publications...

...sharing ideas for the company, but also talking about sports, movies, and just about anything. As our organization...unique lunchtime traditions that might be fun to incorporate into your own lab. Iron Chef-style competitions...easy as possible. However, I think it’s just as important to take a break from work to share time with the...

...make a reporter construct. Fluorescent proteins (FPs) are also the foundation of multiple important technologies... itself is photoconvertible, new studies of spatiotemporal dynamics can be undertaken using available ...

...work was with TALENs, in collaboration with the Porteus lab [2]. With the advent of CRISPR/Cas9, we began...correlative biochemical characteristics and ectopic reporter assays. We have been interested in exploring the...transfect CRISPR plasmids into MEL cells by electroporation, sort your transfected cells via FACS, primer...

..., the Addgene board members, vendors, Addgene supporters, and of course, Executive Director, Joanne Kamens...the Addgene family Perhaps the most heartfelt portion of the entire night was when Melina and Ken called... recognized. John and Stephanie Fan have been supporting Addgene from the very beginning and have played...