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Addgene
Showing: 361 - 380 of 775 results
  1. Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Feb. 25, 2025, 2:15 p.m.
    ...We continue to expand our educational content supporting the tools in our collection, such as blogs describing...viral vector serotypes, or hundreds of Data Hub reports detailing antibody experimental results. We also...exactly what you need, faster. We’re also building a portal to easily share sequences and characterization ...
  2. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ...I’m sitting in the San Francisco International Airport listening to the Lion King soundtrack and writing.... I love running and this event gives me the opportunity to dress up as Mr. Incredible, an added bonus...would like to give my thanks to all the Addgene supporters who spoke with me and deposited plasmids on this...
  3. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    Published
    Jan. 8, 2015, 2:34 p.m.
    ...focus on an aspect of communication especially important for a manager – getting feedback from the team... This can take practice and training. This is important because it creates a culture of engagement in ...  Seeking feedback frequently One of the most important paths of information flow is the one that brings...
  4. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    Published
    Dec. 19, 2013, 3:46 p.m.
    ...over $100 million in annual R&D expenditures to support increased public access to the results of federally-funded...on the global science enterprise lending their support to open scientific research data around the world...online survey of scientists showing overwhelming support for open access to peer-reviewed journal publications...
  5. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    Published
    Nov. 27, 2013, 2:54 p.m.
    ...sharing ideas for the company, but also talking about sports, movies, and just about anything. As our organization...unique lunchtime traditions that might be fun to incorporate into your own lab. Iron Chef-style competitions...easy as possible. However, I think it’s just as important to take a break from work to share time with the...
  6. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...make a reporter construct. Fluorescent proteins (FPs) are also the foundation of multiple important technologies... itself is photoconvertible, new studies of spatiotemporal dynamics can be undertaken using available ...
  7. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    Published
    Feb. 18, 2015, 3:09 p.m.
    ...work was with TALENs, in collaboration with the Porteus lab [2]. With the advent of CRISPR/Cas9, we began...correlative biochemical characteristics and ectopic reporter assays. We have been interested in exploring the...transfect CRISPR plasmids into MEL cells by electroporation, sort your transfected cells via FACS, primer...
  8. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Published
    July 3, 2014, 3:48 p.m.
    ..., the Addgene board members, vendors, Addgene supporters, and of course, Executive Director, Joanne Kamens...the Addgene family Perhaps the most heartfelt portion of the entire night was when Melina and Ken called... recognized. John and Stephanie Fan have been supporting Addgene from the very beginning and have played...
  9. "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

    Type
    Blog Post
    Published
    Dec. 17, 2013, 2:47 p.m.
    ...had certainly realized the importance of seeking out mentoring as an important aspect to their ongoing success...takes us a decade or more for just the “training” portion of our careers. In that time we may have only 2...
  10. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    Published
    July 9, 2015, 2:30 p.m.
    ...a scientist in training can adopt to ensure opportunities in the future. Studies show that the majority...ignore this in their cover letters. It is also important to express interest, even excitement, in a position... to highlight different skills and to get the important information in the right place on the page. 5)...
  11. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    Published
    Sept. 15, 2014, 6:05 p.m.
    ...contribution of students in their labs and the importance of the strong, cross-disciplinary collaboration...Prize. What did Dr. Doudna think was the most important part of their work? First, the discovery of a ... research in the scientific community and the importance of this type of experimentation to the eventual...
  12. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    Published
    April 1, 2014, 5:33 p.m.
    ...Cepko 1. Work hard. There will be periods when opportunities arise. “Grab it!” 2. Don’t get caught up in ... to deadlines, writing up clear and attractive reports, being good at presentations and of course, networking...from heart and hustle. Each PI emphasizes the importance of passion, hard work, collaboration, and staying...
  13. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...components that make up a plasmid and why each is important. Our “Plasmids 101” series designed to educate...and selectively grown on antibiotic plates.  Importantly, because the bacteria from which plasmids are...expression plasmids, gene knock-down plasmids, reporter plasmids, viral plasmids, and genome engineering...
  14. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ...surprising, and important, findings being shared. For instance, recently Cellosaurus received a report that HT...cross-references with cell line catalogs and collections. Importantly, Cellosaurus also identifies problematic cell...
  15. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    Published
    Feb. 16, 2021, 2:15 p.m.
    ...degree is a means to gain employment, so it’s important to make sure a masters helps you get a job you...from the bench, look for “technical or product support specialist,” “field application scientist,” or ... career ladder is larger and there are more opportunities for promotion. It’s not uncommon for bachelor...
  16. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    Published
    July 7, 2016, 2:30 p.m.
    ...worldwide and the presentation made it clear how important it is for us to improve our resources for this...Needless to say we all got very good at applying temporary tattoos on the spot with our trusty spray bottles...microbiology community #ASMMicrobe2016 was a great opportunity for Addgene to let microbiologists know about...
  17. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...therapy - avoiding the use of viruses is also important for social and regulatory reasons. 1.) The transposon...puromycin, hygromycin) and fluorescent protein reporters (GFP, BFP, RFP, no FP). The vectors each contain...region of the RPL41 gene, to drive the selection/reporter parts of each construct. This new tool kit allows...
  18. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    Published
    March 31, 2015, 4:08 p.m.
    ...” instead of levels because it emphasizes the importance of these very different roles. At any stage in...doing, managing or leading. All three roles are important to accomplishing any goal or finishing any project... or highlighting criticisms. To be given the opportunity to take on the role of leader, one needs to take...
  19. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    Published
    Jan. 15, 2015, 1:50 p.m.
    ...attendees an amazing opportunity to share their cutting edge science in increasingly important field.  Interested...through TALENs and CRISPR-Cas nucleases, provided important context for the rest of the day. He was followed...
  20. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    Published
    June 27, 2018, 1:16 p.m.
    ... RNA templates to DNA but suffer from several important shortcomings including low processivity (ie. the...29109157. Nanobody toolkit to monitor retrograde transport at the cell surface Article contributed by Marcy...functionalized nanobodies to study retrograde transport from the cell surface. Their toolkit includes ...
Showing: 361 - 380 of 775 results