We narrowed to 527 results for: set

...its expression, and is flanked (“floxed”) by two sets of incompatible recognition sites (e.g., loxP and...

...different-sized multimers, you may even see different sets of bands on the gel corresponding to each type of...

... discovery of RNA guided transposons in bacteria sets the stage for combining the super powers of CRISPR... transposons encode reporter genes, mutagenic cassettes, or barcodes. When transposons are delivered to...

... done things that I never even imagined before I set foot in the lab. They’re also more than eager to ...

...initiates different immune cascades. In the research setting, antibodies of different isotypes can be used together...of antibodies is used to both identify the cell subsets of interest from among the mixture and to measure...

...large pan-cancer CRISPR-Cas9 gene dependency data sets. Nat Commun 10: . https://doi.org/10.1038/s41467..._style":true,"button_font":{"color":"#FFFFFF","font_set":"DEFAULT","size_unit":"px","font":"helvetica"...nuclease-mediated off-target effects.” Further, large-scale datasets of hundreds of genetic screens using genome-wide...

...addresses these questions, which are critical in setting up a FRET-based assay or screen (the Addgene blog...

...March for Science in Albany, NY, many tables were set up with various science demonstrations, and students...

...microscopist at your institution when heading into a new set of experiments. Many thanks to our guest blogger...number of optical and chemical ‘tricks’ to turn subsets of fluorescent molecules on and off. This can provide...

... easier to automate and use in high-throughput settings, but when it comes to low-throughput applications...recommend avoiding a High Quantity/Low Quality mindset for any applications. DNA quantity is fairly self...reactions with relatively little work after the initial setup. (Addgene actually uses some of these — if you’re...

...anise?” This is something I was expecting when I set out to make my first CRISPR/Cas9 gene edit. I wanted...

... Mello labs also identified several experimental set ups that could improve CRISPR efficiency in C. elegans...introduced. Cre-mediated excision of the selective cassette minimizes the sequence added, leaving a 34 bp ..., however the development of the self-excising cassette (Dickinson et al., 2015) made this step as simple...Engineering with a Self-Excising Drug Selection Cassette. Genetics 200:1035–1049 . https://doi.org/10.1534...genetics.120.303564 Kim H, Ishidate T, Ghanta KS, Seth M, Conte D Jr, Shirayama M, Mello CC (2014) A Co-CRISPR...

...optimize your activation protocol for your particular set up. Applications of photoactivatable fluorescent ...and Wei‐Lih Lee. "Photoactivatable GFP tagging cassettes for protein‐tracking studies in the budding yeast...

...Peripheral Infrastructure Vectors and an Extended Set of Plant Parts for the Modular Cloning System.” PLOS... chromosomal integration of larger expression cassettes (Shaw et al., 2023). Those looking to express ...different promoters, terminators, enhancers, and cross-cassette interactions in gene expression, and to generate...

...Gersbach Lab multiplexing plasmids: This plasmid set allows you to express 2-4 gRNAs, with four being ...including Standard PolIII-gRNA cassette, Csy4-cleavable cassette, and PTG cassette.    Find gRNA multiplexing...smaller than constructs with multiple promoter-gRNA cassettes, making them advantageous for small capacity vectors...aTet-inducible gRNA and a backbone-targeting gRNA cassette for plasmid curing through "self-destruction" ...

...optimization before the field begins to converge on a set of standard tools. Additionally, aspiring genetic...

...You’ll want to select a pre-stained or unstained set of standards that covers a size range appropriate...

...vasculature (adult marmosets), biased toward astrocytes (adult rhesus macaque) Rats, marmosets, green monkeys... including ENS (adult marmosets, infant rhesus macaques) Mice, rats, marmosets, rhesus macaques Unknown... screened in marmosets. AAV.CAP-Mac effectively transduces vasculature in adult marmosets (Chuapoco et...transduction levels in marmoset brain, with both neurons and astrocytes targeted. While marmoset liver transduction...AAV.CAP-B22 CNS: neurons and astrocytes Mice, marmosets Unknown Goertsen, Flytzanis, et al., 2022 ... of inhibitory and excitatory neurons Mice, marmosets Unknown Brown et al, 2021 Goertsen, Flytzanis...

...side of a stop cassette, upstream of an effector gene. In cells where the stop cassette is removed during...just a fraction of a single genetically-defined subset of cells. By labeling fewer cells in a population...cells will express the effector or retain the stop cassette following recombination. Image from Isaacman-Beck... genetic tool that allows for the labeling of a subset of cells with the same genetic makeup, overcomes...effector is expressed. In cells where the stop cassette remains, the effector is not expressed and the...attP sites to drive expression of either a stop cassette or an effector gene. HDVR (2X Hepatitis Delta ... Truncating the attP site upstream of the stop cassette results in decreased efficiency of recombination...

... satellite colonies? Ampicillin Fair {% icon icon_set="fontawesome-5.14.0" name="Check" style="SOLID...decorative" title="Check icon" %} Just bacteria {% icon icon_set="fontawesome-5.14.0" name="Check" style="SOLID...Excellent No Bacteria and eukaryotes (G418) {% icon icon_set="fontawesome-5.14.0" name="Check" style="SOLID...purpose="decorative" title="Check icon" %} {% icon icon_set="fontawesome-5.14.0" name="Check" style="SOLID...Check icon" %} Carbenicillin Excellent {% icon icon_set="fontawesome-5.14.0" name="Check" style="SOLID...