We narrowed to 697 results for: tec

...the Dow lab created a base editing reporter that detects and enriches for base editing events in vivo without...conversion) they could induce the translation of any detectable protein, not just fluorescent proteins. ...protein expression, it can be used for an array of detectable proteins including fluorescent proteins, antibiotic...

...the screen. This toxicity was used a proxy for detecting Cas9 cutting. Safe targeting guides better control...off-target cleavage by CRISPR-Cas nucleases." Nature biotechnology 33.2 (2015): 187. Pubmed PMID: 25513782. Pubmed...specificity using truncated guide RNAs." Nature biotechnology 32.3 (2014): 279. Pubmed PMID: 24463574. Pubmed...

...A puromycin resistance gene on your vector can protect both plasmid-carrying bacteria and eukaryotes. ...protein synthesis, and the neomycin resistance gene protects against any of them. But different aminoglycosides...host range plasmids shuttle vectors? While it’s technically accurate to say that shuttle vectors have a “...

...editing in mammalian cells and embryos. Nature Biotechnology, 42(4), 638–650. https://doi.org/10.1038/s41587...with an N-methylpurine DNA glycosylase. Nature Biotechnology, 41(8), 1080–1084. https://doi.org/10.1038/s41587...and C-to-G editing in mammalian cells. Nature Biotechnology, 42(10), 1538–1547. https://doi.org/10.1038/...

...expression. Trends in Biotechnology 22:346–353 . https://doi.org/10.1016/j.tibtech.2004.04.006 Hausser ... be used in some in vivo experiments and in biotechnology and therapeutics (Mauro and Chappell 2014).  ...

...have a natural preference to stay in dark areas (protected) as opposed to brightly illuminated ones (risky...environments by initially moving along a wall (protected) rather than being out in the open (risky). Taking...comprised of a brightly lit (risky) and a dark (protected) area. When measuring the time spent in a particular...

...split-Cas9 architecture for inducible genome editing and transcription modulation. Nat Biotechnol 33:139–...discovery through the creation of innovative technologies. To date his group has develop several popular...

...polymorphisms specific to their origins. This technique is still used today to test for HeLa contamination...test is so good that it is used as a forensic technique (aka DNA fingerprinting) that can place suspects...stance on the matter. Funding bodies such as the Biotechnology and Biological Sciences Research Council (BBSRC...

...ability of reviewers (and, once published, anyone) to detect possible errors in assumptions, use of inappropriate...senior colleagues. Secondly, science is highly technical and this has a natural, albeit unintentional, ...Policy: Part Two U.S. Office of Science and Technology Policy (OSTP) memo Increasing Access to the ...

...told all of the nuances of the research in as technical of language as possible. Seriously. My suggested...review of your field or the finer points of your techniques. You DO need to be prepared to know the literature...searches. I've updated chapters to reflect new technologies, i.e. video interviews. For point of reference...

...with the contents of the two AAVs. Using this technique, Bok and Porteus deliver a repair template that...respectively. Future researchers could apply this technique to efficiently deliver large repair templates ...AAV vectorization of DSB-mediated gene editing technologies." Current gene therapy 16.3 (2016): 207-219....

...keeping. Top priorities in most cases include IP protection, publications and funding. If any of these three...witnessing and audit trails. How does the ELN provider protect and respect your IP rights and data ownership? ...interested in combining scientific research with tech advances. You an follow SciNote on Twitter @scinoteELN...

...David Liu’s lab by combining Cas9 DNA-targeting technology with a fused cytidine deaminase to create C→T...activity and therapeutic application. Nature Biotechnology, 38(7), 892–900. https://doi.org/10.1038/s41587...Cas domain compatibility and activity. Nature Biotechnology, 38(7), 883–891. https://doi.org/10.1038/s41587...

...it comes to altering gene expression, CRISPR technologies have gradually taken over as the preferred method... their promoters in human and mouse. Nature Biotechnology, 35(9), 872–878. https://doi.org/10.1038/nbt... constructing shRNA expression vectors. BMC Biotechnology, 6(1). https://doi.org/10.1186/1472-6750-6-1...

.... Alternatively, you can perform simultaneous detection with a mixture of primary antibodies and appropriate..., M., McDonough, L., Zhu, Y., Neumann, E., De Caestecker, M., Gutierrez, D., & Spraggins, J. (2022). Multiplex...Introduction to Antibodies Which Fluorescence Microscopy Technique is Best for Me? Introduction to Immunofluorescence...

...repair of the break to allow for site-specific detection downstream. The con to these approaches is that...that NHEJ must perform the repair to allow for detection, not Homologous Recombination or Alternative End...effects in CRISPR/Cas9 gene editing. Front Bioeng Biotechnol. 2023 Mar 9;11:1143157. doi: 10.3389/fbioe.2023.1143157...

...the system, researchers have used split GAL4 architecture or combined the system with Flp/FRT, as reviewed... regulated expression in mammalian cells. BMC Biotechnol 6, 43 (2006). https://doi.org/10.1186/1472-6750...expression system for Bacillus. Appl Microbiol Biotechnol. 2019 Jan;103(1):303-313. doi: 10.1007/s00253...

... Why might one be interested in state machine technology? Biological research has produced a massive amount...itself to cheap, quick, and high throughput state detection with flow cytometry. However, expressing reporters...synthetic circuits from genetic context." Nature biotechnology 30.11 (2012): 1137-1142. PubMed PMID: 23034349...

...developing an easy to use diagnostic tool for the detection and diagnosis of different types of pancreatic...trying to get hands-on lab experience is that the technology is so advanced that most of the procedures in...own challenges. I’ve had to become skilled with technology like Skype in order to have meetings between ...

... good correlation between the levels of target detected in both methods. These approaches have relatively...are ultimately planning to use your antibody to detect endogenous levels of the target in other cells ...Antibody validation: A view from the mountains. New Biotechnol 45:1–8. https://doi.org/10.1016/j.nbt.2018.08.002...