We narrowed to 783 results for: INA

... but among the most important are the inverted terminal repeats (ITRs). ITRs are the elements that make...and the ITRs are retained with the gene in a recombinant AAV (rAAV) vector (Figure 1). Everything else...outside the ITRs gets left behind.    Figure 1: Recombinant AAV vector with ITRs that flank the gene cassette... Rep78 and Rep68 to initiate replication and a terminal resolution site that serves as the target site...T-shaped structure. The Rep binding element (RBE) and terminal resolution site (trs) are noted. Created with ...serotypes (Wilmott et al., 2019), and different combinations of ITR and capsid serotypes vary in their gene...plasmid DNA with mutated ITR sequences. Using recombinant-deficient bacterial strains such as NEB Stable...

...recently announced the launch of an open-access recombinant antibody resource called NABOR (Neuroscience ...The antibodies distributed through NABOR are recombinant antibodies expressed from plasmids that have ...RNA could then be used to generate functional recombinant monoclonal antibody (R-mAb) expression plasmids...preserve and share their own collections. Why Recombinant Antibodies?  One of the benefits of using a plasmid...including subclasses that were not produced by the original hybridoma. By using subclass specific secondary...Multiplex immunolabeling with subclass-switched recombinant antibodies in adult rat brain. Figure from Andrews...publicly available. The lab has also expanded their recombinant affinity reagent tools to include nanobodies....

...libraries containing combinations of different gRNAs in each plasmid. Each gRNA combination can be tracked ... an easy-to-use tool to analyze the complex combinatorial genetic networks underlying your favorite biological...screening are vastly useful for mapping the combinatorial genetics that underlie complex regulation in...barcoded gRNA libraries that can be used to combinatorially modify the genome, screen for a particular ...successfully applied to functionally characterize combinatorial gene knockouts generated via multiplexed gRNA...expression (Wong et al., 2016), in addition to the combinatorial expression of other genetic elements including... Figure 2: Strategy for assembling barcoded combinatorial gRNA libraries. (Adapted from Wong et al., 2016...

... or gene, as well as to study the effects of inactivating/activating mutations. In biomedical research.... In recombineering, bacteria express phage recombination machinery, which facilitates easy genetic modification...modification by homologous recombination. In most recombineering strategies, only 3-4 phage proteins are...a temperature-sensitive plasmid. Homologous recombination then joins the linear PCR products into stable...the recombineering plasmid is not maintained. Recombination efficiency is high enough to negate the need...own electrocompetent cells in which the phage recombination machinery is induced using L-arabinose. These... the steps of gel purification and in vitro recombination. Across all modifications described in Trehan...

... and increase your lab sustainability. For my other posts on lab sustainability, check out my Recycling...This is the last post in a blog series on sustainable practices in the lab, where I’ll list some easy...unimportant. With so many big contributors to the sustainability problem, how can one little change make a difference...quick things above, try joining or starting a sustainability project. This could be: Setting up a recycling...Learn about other creative approaches for lab sustainability Resources on Addgene.org Sign up for our ...

...maintaining intensity at ~85% of its original value after 60 minutes of illumination, highlighting its reliability...scientists. Seeing inside these specimens has offered illumination on biochemical processes that are crucial in...interpretation of FRET-based experiments. A popular combination is Alexa594, and Alexa647 due to their brightness...

... you encounter mycoplasma contamination. Causes of mycoplasma contamination The mycoplasmas enter the ...above. The contaminants can spread through dirty clothing, lab wears, human speech near the laminar airflow... risk of contamination. Mycoplasmas can spread from these sources through cross-contamination and due ... a source of mycoplasma contamination. Due to its turbid nature, contamination is difficult to be detected...during working in the laminar airflow are also among the leading causes of contamination. These aerosols generated...cell culture process and hence eliminate one of the causes of contamination. If possible, a separate lab...bacterial and fungal contamination. Proper PPE Since humans are the major source of contamination, the prevention...

...vectors, recombination often occurs between the long-terminal repeats (LTRs). After recombination, one resulting...intramolecular DNA recombination. This naturally occurring process is mediated by recombinases like RecA and...undergo recombination, two smaller plasmids are formed. Viral vectors are prone to recombination In lentiviral...replicate.   How can I prevent recombination in my samples? Plasmid recombination can affect viral vectors ...have been engineered to further reduce recombination. Recombinase-deficient strains like Stbl2, Stbl3, ... repeating elements. Knowing how to amplify recombination-prone plasmids and testing your prep can save..., and NEB stable E. coli reduce the rate of recombination in samples. For a more detailed comparison of...

...to avoid MMR: 1) E. coli with inactivated MMR: Using E. coli with inactive MMR is definitely the easier...minutes quickly inactivates the repressor and allows for expression of the recombination genes.  After ...confirmation of recombinant clones If a antibiotic resistance gene has been inserted, recombinants can first...genome engineering and is based on homologous recombination. It allows for direct modification of DNA within...called recombineering - short for homologous recombination-mediated genetic engineering.  It can be used... These enzymes then catalyze the homologous recombination of the substrate with the target DNA sequence...nucleotides of homology to the target site for recombination. Key components of the Lambda Red recombineering...

...however, the other recombinase/reporter pairs were inactive, even at very high recombinase levels. These data...multiple recombinases can be used together to designate specific patterns of recombination. Finally, Rubin...rather than complete recombination. If you’re looking to use site-specific recombination in Drosophila, read... using multiple recombinases. To make multiple, precise genome edits, the recombinases used must have ...representing the specific recombination site for each enzyme, were generated to test recombination efficiency. When...showed broad recombinase-dependent RFP expression. Even at low expression levels, recombination by KD, B2...determine the cross-reactivity of these recombinases, each recombinase and reporter construct pair were tested...