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We narrowed to 320 results for: POR C

Showing: 11 - 20 of 320 results

  1. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...fourth-generation base editors, BE4, reduce the undesired C→G or C→A conversions that can happen with BE3s. These...Compared to BE3, BE4 offers a 2.3 fold decrease in C→G and C→A products as well as a 2.3 fold decrease in indel... Hong, M., Zhang, D., Luan, C., Zhang, S., Wu, H., Gao, H., Bai, S., Li, C., Ding, R., Xue, N., Lei, Z... W., Levy, J. M., Yeh, W.-H., Zheng, C., Newby, G. A., Wilson, C., Bhaumik, M., Shubina-Oleinik, O., Holt...) and adenine base editors (ABEs). CBEs mediate a C to T change (or a G to A change on the opposite strand...strand). ABEs make an A to G change (or a T to C change on the opposite strand). Both are base transition...excision repair, which would change the U back to a C base. To increase base editing efficiency, Komor and...

  2. The Advances Behind The World's First Personalized CRISPR Treatment

    Type
    Blog Post
    Published
    Sept. 16, 2025, 1:15 p.m.
    ...Robinson-Garvin, N. A., Small, J. C., McCague, S., Burke, S. M., Wright, C. M., Bick, S., Indurthi, V., Sharma...technology with a fused cytidine deaminase to create C→T base edits at a target locus. A year later, an A...plagued CRISPR editing at the time. KJ was born with a C→T mutation on one allele of his CPS1 gene, which could...necessarily mean researchers can fix any problematic C or A mutations in the genome. Off-target effects, ....1126/science.1231143  Gaudelli, N. M., Komor, A. C., Rees, H. A., Packer, M. S., Badran, A. H., Bryson... R. (2017). Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage. Nature, 551(7681...Liquori, A. J., Murray, R., Packer, M. S., Rinaldi, C., Slaymaker, I. M., Yen, J., Young, L. E., & Ciaramella...

  3. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ... with 40–60% G/C content are most likely to be successful, although sequences with G/C content outside...of the 3′ extension of the pegRNA should not be C. A C base is speculated to base pair with G81 of the..., J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson, D., Zhao, A.,...Figure 3: pegRNAs whose 3′ extension begins with a C base can cause unwanted base pairing (left), while... Koblan, L. W., Levy, J. M., Chen, P. J., Wilson, C., Newby, G. A., Raguram, A., & Liu, D. R. (2019). ... Knipping, F., Ravisankar, P., Chen, P.-F., Chen, C., Nelson, J. W., Newby, G. A., Sahin, M., Osborn, ..., Anzalone, A. V., An, M., Newby, G. A., Chen, J. C., Hsu, A., & Liu, D. R. (2022). Engineered pegRNAs...

  4. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...editing toolkit to allow C to U edits using their RESCUE (RNA Editing for Specific C-to-U Exchange) system...RESCUE-S) offered ~76% on-target C-to-U editing while reducing off-target C-to-U edits by ~45% and off-target...lab published a paper on an RNA editor that allows C to U edits (RESCUE). We’ll walk through how these ...adjusted the structure of the guide RNA by introducing a C opposite the target A to be edited. This change specifies...residues that contact the RNA substrate resulted in 15% C-to-U editing activity. They then performed sixteen...cytidine deaminase activity. While RESCUE can perform C-to-U edits, the authors note that it retains its adenine... that RESCUE is most active in a 30 nt guide when C or U base-flips are present across from the target...

  5. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...in fact, permeable to CO2 at low temperatures (-80°C) and once in the tube, the CO2 can alter the pH of...pH indicator phenol red. We froze samples in a -80°C freezer and then moved them to a closed dry ice storage...samples to controls that were only stored in a -80°C freezer and not exposed to CO2/dry ice. All samples...then tested whether returning the tubes to the -80°C freezer after storage on dry ice allowed the CO2 to... (Fig.4). To test this, we froze the tubes at -80°C and then moved them to closed storage on dry ice for...for 48 hours. We then moved the tubes back to a -80°C freezer for various recovery periods between 0 and... samples to controls that were only stored at -80°C. We then thawed the tubes after the indicated incubation...

  6. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    Published
    July 7, 2020, 1:15 p.m.
    ...expressed to identify successfully transfected cells. C-to-T editing by the APOBEC-Cas9 editosome would restore...codon that is converted to a ‘TAC’ or ‘TAT’ after a C-to-T base editing event. This edit changes the histidine... ATG codon from an ACG codon using a base-editor (C>T conversion) they could induce the translation of...Liu DR (2017) Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage. Nature 551:464... Figure 4: Reporters in Gene On include a mScarlet reporter, luciferase reporter, and neomycin resistance... editing. eGFP reporters for single base editing by APOBEC-Cas9 Though the ACE reporter quantifies APOBEC-Cas9...DSB in their previous reporter, the Harris Lab created a panel of eGFP reporters to quantify the on-target...

  7. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    Published
    April 9, 2019, 1:13 p.m.
    ...fluorescent protein is at the C-terminus of the POI or that the POI is at the C-terminus of the fluorescent...dimerization (Shaner et al., 2008 - supplementary figure C). However, these assays are qualitative. The Organized...structural information available, it’s clear that the C-terminus of fluorescent proteins derived from Aequorea...biosensors (yellow cameleon 3.60, EPAC, and Galphai), the C-terminus and N-terminus of the donor fluorescent protein...the FRET efficiency. Creating fusions at the N- and C- termini of your protein of interest The most straightforward...straightforward fusion protein is made by connecting the N- or C-terminus of the fluorescent protein and protein of... the sequence, people often use terminology like “C-terminal fusion,” but I find this very confusing because...

  8. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ... Lucia, C., Federico, P.-B., & Alejandra, G. C. (2020). An ultrasensitive, rapid, and portable coronavirus...Time: ~40 minutes Equipment required: 37°C heat block and 62°C heat block Approach: Viral RNA is extracted...range Time: ~1 hour Equipment required: 37°C water bath and 42°C water bath Approach: RNA is extracted from...Valois, E., Solley, S. C., Braig, F., Lach, R. S., Baxter, N. J., Kosik, K. S., Arias, C., Acosta-Alvear, D...incubation  Time: ~90 minutes Equipment needed: 37°C heat block, LED blue light illuminator or UV light... in a range of temperatures varying between 10-50°C. Visit Addgene's COVID-19 Plasmids and Resources ...Sotomayor-Gonzalez A, Zorn K, Gopez A, Hsu E, Gu W, Miller S, Pan C-Y, Guevara H, Wadford DA, Chen JS, Chiu CY (2020) ...

  9. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...j.cell.2021.09.018 Choi, J., Chen, W., Suiter, C. C., Lee, C., Chardon, F. M., Yang, W., Leith, A., Daza,..., J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson, D., Zhao, A.,...Leukemia Virus (M-MLV) reverse transcriptase to the C-terminus of Cas9 H840A nickase (PE1). But innovations...pegRNAs (Yan et al., 2024). The team fused La to the C-terminal end of PEmax to produce PE7. PE7 significantly...expression ~ ~ ~ ~ ✓   ✓ SV40 and c-Myc nuclear localization sequences Improve translocation...References Anzalone, A. V., Gao, X. D., Podracky, C. J., Nelson, A. T., Koblan, L. W., Raguram, A., Levy... Koblan, L. W., Levy, J. M., Chen, P. J., Wilson, C., Newby, G. A., Raguram, A., & Liu, D. R. (2019). ...

  10. Bringing Sustainable Practices to the Lab: Easy Action Items

    Type
    Blog Post
    Published
    April 22, 2019, 12:26 p.m.
    ...institute. Set your -80 °C freezer to -70 °C and teach other labs to do the same. -70 °C is cold enough to keep...years. In fact, some scientists speculate the -80 °C setting might have been a marketing campaign! MIT ...biological samples that are stored long term at -70 °C. My Green Lab also has resources and even a freezer... list some easy but effective habits you can incorporate to reduce your lab consumption and increase your...in your daily habits can feel ineffective or unimportant. With so many big contributors to the sustainability...
Showing: 11 - 20 of 320 results