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... Clinical applications Viruses in vaccines helped pave the way for their use in other clinical applications...etc.). When you’ve decided on an application, our Viruses as Biological Tools blog can help you learn about...vector from basic science research all the way to clinical gene therapy. But how can you use these amazing...s unpack the myriad number of emerging viral applications and give you some recommendations for putting...putting viruses to work. Viral applications Viruses work by infecting a host cell (the target of viral infection...and delivering a genetic payload. This cargo is typically their own genome, but this step can be engineered...expressed. It’s important to note that for all the applications we discuss, there is more than one type of virus...

...– The basics Statistical analysis is what determines if there is statistical significance among data sets...values – numerical risk allowance in a statistical test. Distribution - spread of data; typically, normal...asterisk of significance? You’ve already done the heavy lifting, so don’t let the statistical analysis of... sets. Significance is a numerical value which quantifies the likelihood that a result is due to chance...are statistically different. They are classic parametric tests and one of the simplest statistical tests...analysis on a five-sample data set yields statistical significance, you won’t know if only two of those samples...if the dependent variables worked synergistically, antagonistically, were additive, etc.). ANCOVA and...

...xkcd.com Science communication should be fun and engaging for the communicator as well as the audience...many ways options available to today's science communications: podcasts, blogging, videos, lectures, exhibits...in what people think of as "the best way" to communicate their science, meaning that they will heavily...heavily evangelize for the mode of communication they connect best to. And that's really natural - if it works... text or use Facebook. However you choose to communicate your science, some people will love it and others...audience, so pick a mode of communication that you enjoy and that plays at least slightly to your strengths...outside of the box. There are so many ways to communicate and I wholeheartedly encourage experimentation...

...extension is repeated 25-35 times to exponentially replicate the target DNA of interest. This entire reaction...have been developed for different scientific applications. These PCR methods all use the same basic PCR...not as quantitative as other methods of PCR. Theoretically scientists should be able to determine the quantity...the final cycles which would slow or stop PCR amplification. Other times, the PCR reaction may not be 100%...quantified by qPCR. qPCR is used in a variety of applications including gene expression profiling, studying...concentration, unlike qPCR where results can vary across replicates. ddPCR can be used to quantify DNA sequences ...droplet contains the material required for PCR amplification. Following the PCR reactions each droplet is...

...have such epitopes. These classical 'undruggable' targets are often intrinsically disordered and structurally...suppressors to growth factors, some of the most clinically-relevant proteins are also the hardest to study... detection of proteins without the challenges typically occurring with a fluorescence-based approach. ...species that can lead to phototoxicity, perturb biological processes, and compromise cell viability (Laissue...overcomes these challenges and enables precise quantification with with nothing more than substrate addition...real time. Let's take a closer look at several applications of this technology. In each section below, we...ubiquitin ligases in the degradation of CFTR, specifically UBE3C. Degradation of CFTR was measured through...

... For the clean-up stage, plasmid purification kits typically use one of two common approaches — columns...column-based DNA purification methods perform well in terms of quality and yield. Bead-based purifications tend to...plates, and yes those purification columns we’ve been talking about!) are a significant source of waste in...reduce the environmental impact. Using non-silica-based purification protocols requires less plastic and avoids...Minimized amount of silica ACT environmental impact factor label Non-silica based, avoids use of...standard purification kits, there’s a range of specialty kits available for plasmid purification. Kits can... Some manufacturers offer kits specifically optimized for purification of these large plasmids, while ...

...the chemical factor, of a naturally occurring voltage indicator. To give an example, a genetically encoded... Gong Y. Enhanced genetically encoded voltage indicators advance their applications in neuroscience. Curr...and sensing electrical potential. Thankfully, there is a tool to measure these electrical events! Voltage...Voltage indicators are voltmeters for cells! In this blog we will review how these indicators work, their...their general applications, and why viruses are essential to their use as biological tools. Why Use Voltage...Voltage Indicators? Electrical signals are integral to many facets of biology. For example, many heart issues...studying voltage within a cell are genetically encoded voltage indicators (GEVIs) and chemigenetic sensors...

...and includes biochemical properties, previously established uses, and intended applications. Within CasPEDIA...CasPEDIA, there is a classification system, CasID, to help facilitate direct comparison of different enzymes...multiplex ability Experimental guidelines and applications Protein structure and properties Links to ...you want to read literature on the less common applications of an enzyme for your non-traditional experiment...al. (2023). CasPEDIA Database: A Functional Classification System for Class 2 CRISPR-Cas Enzymes [Preprint...I., Iranzo, J., et al. (2019). Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and...

...after application of NE (10 μM) and corresponding pixel-wise ΔF/F0 heatmaps. White insets indicate surface...developing and depositing two new norepinephrine indicators: nLightG and nLightR (Kagiampaki et al., 2023...surface expression of the indicators over white dashed lines. Scale bars, 10 μm (HEK293T), 20 μm (neurons...trialed in two-photon in vivo experiments. nLight indicators are more sensitive to and selective for norepinephrine...feedback in response to norepinephrine release, and clinically-used drugs targeting these receptors cannot be...They can, however, be studied with the nLight indicators. Speed One of the major advantages of this next...nLightR, both generating a measurable signal after electrical stimulation with a single pulse, while nLightR...

...names for technical support roles. Examples are Technical Service Representative, Technical Support Specialist...these departments from technical support. Technical support careers in brief Technical support careers are...troubleshoot problems at the bench. Specifically, roles in technical support make excellent use of the ...worked as a technical support specialist for 4 years. Read on for insights from my time in technical support...products successfully. In technical support you may have to answer technical enquiries such as: “Can I... areas of expertise in technical support positions. When applying for technical support positions, you...familiarize yourself with technical support. Even having personal experience with technical support through the...