Choosing Your Fluorescent Proteins for Multi-Color Imaging
Type
Blog Post
Published
Oct. 9, 2014, 3 p.m.
...both mCherry and TagRFP.
Here, the TagRFP spectrum is shown in the darker colors and the mCherry spectrum...larger fraction of the TagRFP emission than the mCherry emission. For this filter set, we would expect ...expect TagRFP to give a brighter signal than mCherry. In general, filter sets designed for Rhodamine / Cy3 ...or mRuby2 than longer wavelength proteins like mCherry. For background on fluorescence and filter sets...