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We narrowed to 148 results for: plasmid repository

Showing: 41 - 80 of 148 results

  1. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...at Emory recently contributed a set of plasmids to the repository that will help epigenetic researchers...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head...head over to our CRISPR Plasmids and Resources page.  Plasmids for delivering CRISPRi components by a single...inducible degron system for C. elegans New CRISPR plasmids New items from the viral service Epigenetic ...researchers custom design their own engineering plasmid tools in about a week. Taking advantage of Golden Gate ...

  2. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ... mission is to enable that process. Our repository of plasmids, AAVs, antibodies, and data allows scientists...that Addgene’s mission statement extends beyond plasmids, AAVs, and antibodies to our content, website,...and cited figures from papers or image sharing repositories. Then we format, do a final review to catch ...Resources on the Addgene Blog Science careers Plasmids CRISPR Viral vectors Fluorescent proteins  ...

  3. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...Addgene Blog: Plasmids 101: How to Verify Your Plasmid Plasmids 101: An Inside Look at NGS Plasmid Quality ...results of the plasmids in our repository with known reference sequences, such as full plasmid sequences provided...deposit their plasmids with us or other entries in NCBI’s numerous databases. As our repository has grown ...up their plasmid and cloning verification steps and free up more time for using your plasmids instead!...Snapgene-powered plasmid maps. Resources on Addgene.org Sequence Analysis of your Addgene Plasmid Addgene Plasmid...grown over the years (we now have over 60,000 plasmids!), the number of sequencing results we analyze as part...busy week, we may need to analyze more than 200 plasmids as part of our quality control process. Consequently...

  4. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    Published
    Feb. 1, 2024, 2:15 p.m.
    ...materials! Some of the materials in our repository, including rabies plasmids, are export-controlled materials... ship rabies plasmids and viral vectors containing genetic elements from rabies plasmids to international...export license applications for VSV and SARS-COV-1 plasmids. Export control license process    An export ...license, once approved, allows Addgene to ship plasmids or viral vectors containing sequences from rabies...  Timelines  We have offered VSV and SARS-COV-1 plasmids under this application process since May 2020....at [email protected].  Curious about how rabies plasmids are used in the lab? Check out our “Rabies and...browse tools for AAV tracing, made from rabies plasmids deposited by Ian Wickersham’s lab.   ...

  5. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ... first CRISPR plasmid deposit. Now we have over 8,000 CRISPR plasmids in the repository that have been...

  6. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    Published
    Nov. 14, 2013, 4:10 p.m.
    ...scientists about Addgene’s repository, and explaining how to deposit and request plasmids, but I also spend a...scientists (and our growing collection of 25,000+ plasmids from 1,600+ labs around the world!) that have ...inside scoop from scientists who have deposited plasmids at Addgene.  Learn how discoveries were made and... and get practical tips on how to use the plasmids in your own lab. Summaries of the latest technologies...the bench, with a special focus on cloning and plasmid-based technologies. Commentary about scientific...

  7. Activate Addgene's "Find Plasmids" Badge on Pubmed Abstracts

    Type
    Blog Post
    Published
    May 12, 2017, 12:58 p.m.
    ...Addgene “Find Plasmids” badge in the image below. If an abstract on PubMed has the “Find Plasmids” badge, you...you’ll find new plasmids. Simply click on the badge to be taken directly to the plasmids on Addgene’s site... Icon” box next to the “Addgene Nonprofit plasmid repository” choice. This selection should populate your...you should see the “Find Plasmids” box on papers that have deposited plasmids. Now that you know how ...Order Plasmids from Addgene Check Out Our FAQ Pages Browse Plasmids ...thought - Is there an easy way to find published plasmids? Do you spend a lot of time in PubMed searching...you can use PubMed to see which articles contain plasmids deposited at Addgene from the abstract page. Check...

  8. Depositor Week 2020 is Happening Now. Join us!

    Type
    Blog Post
    Published
    Nov. 16, 2020, 2:15 p.m.
    ...are over 2,300 COVID-19 related plasmids currently in Addgene’s repository which have been requested about...While the bulk of these plasmid deposits came in this year, there were some plasmids deposited as early as... We started from 474 plasmid deposits in 2014 which has grown to ~10,000 plasmid deposits per year in ...the scientific community the latest and greatest plasmid tools! Thank you depositors! In fact, this year...the GSA’s blog Genes to Genomes about a TMPRSS2 plasmid from Roger Reeve’s lab as an example). Depositor... of the Addgene community. Perhaps you’ll find plasmids and new tools that will speed your science along...and trivia begins! Tuesday: Depositor features (plasmid deposits and blog posts showcasing the work of ...

  9. New Viral Vectors - Winter 2025

    Type
    Blog Post
    Published
    Jan. 28, 2025, noon
    ...new preps available in the Addgene viral vector repository with a nice cup of hot chocolate is absolutely...New ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor  Notes ...service, which offers over 3,000 eligible transfer plasmids and five different serotypes. Not sure what to...

  10. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    Published
    May 17, 2017, 3:37 p.m.
    ...protocols, and, of course, plasmids. Today we’re trying a new way to present plasmid info with a new segment...The Hot Plasmids Segment. Click on the player below to listen to a quick (~5 min) Hot Plasmids podcast... new plasmid technologies from one of our recent newsletters. Listen to the entire Hot Plasmids episode...greatest plasmids through this podcast segment. Below, we break down each of the Hot Plasmids segments...articles on the Hot Plasmid webpage. We hope you enjoy this new way to learn about plasmid technologies. If...comments about the Hot Plasmids Podcast Segment, or have recommendations for new Hot Plasmids topics, let us ... comments section below. Hot plasmids covered in this segment Hot Plasmid 1: CRISPR X from Michael Bassik's...

  11. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...received over 13,000 plasmids requests for COVID-19 related plasmids. These plasmids have reached labs in...small collection of plasmids for coronavirus research, including a handful of plasmids that have been in...in the repository for many years, has now grown into a collection of over 2,400 plasmids, with many available...some highlights of the plasmids shared over the last year and how these plasmids have been used to accelerate...circulating and plasmids containing these mutations have been deposited at Addgene. Plasmids deposited from... labs also deposited plasmids for producing COVID-19 diagnostic enzymes. Plasmids in this toolkit include...These plasmids contain both untagged and tagged versions of SARS-CoV-2 ORFs and include plasmids that ...

  12. Addgene’s Top 10 Blog Posts from 2014

    Type
    Blog Post
    Published
    Dec. 16, 2014, 8:01 p.m.
    ...seen through the lens of Addgene’s blog and plasmid repository. Our blog is just over a year old, and it...loved helping to answer your plasmid and cloning questions through our Plasmids 101 series and by responding...Melina Fan Plasmids 101: The Promoter Region – Let's Go! By Kendall Morgan Plasmids 101: Mammalian...Joanne Kamens Plasmids 101: Antibiotic Resistance Genes By Marcy Patrick Plasmids 101: Yeast Vectors...FAQs Answered! By Caroline LaManna Plasmids 101: What is a plasmid? By Margo R. Monroe Best wishes...detailed information about this new technology. Our Plasmids 101 series continues to grab scientists’ attention...Genome Editing By John Doench (Guest Blogger) Plasmids 101: Origin of Replication By Kendall Morgan ...

  13. New Educational Resource: The Addgene Videos Page

    Type
    Blog Post
    Published
    June 16, 2017, 2 p.m.
    ... or lab process. Along with our role as a plasmid repository, Addgene aims to be a vital educational resource...Addgene.org Check Our Our Protocol Pages Browse our Plasmid Reference Pages ...

  14. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    Published
    Sept. 25, 2018, 2:39 p.m.
    ...a million plasmids would fill? Two weeks ago, Addgene edged passed our one million plasmids shared milestone...ending the day with 1,000,002 plasmids shipped. Reaching one million plasmids shared might have you wondering...how many plasmids leave Addgene headquarters every day. That would be an average of 673 plasmids each day... without the scientists who deposited plasmids to the repository and for the scientific sharing community...to packing and shipping. Every single day. 673 plasmids shipped daily may be easy to picture (see the ...celebratory lunch, it’s hard to picture one million plasmids. How much space would one million bacterial stabs...post, we looked at how long it takes to make one plasmid and how much time Addgene has saved researchers...

  15. More Data for You: Find Articles Citing Addgene Plasmids

    Type
    Blog Post
    Published
    June 16, 2015, 2:15 p.m.
    ... from Addgene Plasmids 101: How to Verify Your Plasmid Plasmids 101: Control Plasmids 6 Tips for Analyzing... on our plasmid pages - links to articles citing this plasmid. Now you can learn how a plasmid has been...your deposited plasmids Increased visibility of your plasmids in the "Associated Plasmids" lists Benefits...Find other related plasmids in the "Associated Plasmids" lists Properly citing plasmids you've requested...Citing This Plasmid" list. Email us at [email protected] with the Plasmid Name, Addgene Plasmid ID #, and...'ll find the plasmids listed below the citation. An example of these "Associated Plasmids" is shown in...explore the TetO-FUW-OKSM plasmid page. Or search for your favorite plasmids and see where they've been...

  16. Download Science-Themed Zoom Backgrounds from Addgene

    Type
    Blog Post
    Published
    April 22, 2021, 1:15 p.m.
    ...lentiviral plasmids include popular envelope and packaging plasmids as well as transfer plasmids. Download...favorite reagent, scientific technique, or plasmid repository at your next meeting.     If you’re reading...Otherwise, check out our plasmid and DNA backgrounds. Download the blue plasmid background.     You can... the plasmids that Addgene offers by visiting our collections page. Here, you can browse plasmids by field...cute mascots!  Blugene has been helping us make Plasmids 101 videos and needed a break so they're hanging...coronavirus collection page, where you can find plasmid tools to accelerate your COVID-19 research.  Download...background.         If you’re predominantly a plasmid user, instead, don’t fret; we have backgrounds ...

  17. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...switch. Zhang’s team devised the plasmids now available in Addgene’s repository for use in neurons, both in...biological processes and disease states. While LITE plasmids have clear application to anyone with an interest...

  18. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    Published
    April 11, 2023, 4 p.m.
    ...insight to the most requested plasmid, the number of requested plasmids, or trends of requests over time...website, allowing depositors to download their plasmid request data, as a CSV file, anytime. The CSV is... Visualizing the data So, after downloading my plasmid request data, I started to explore how I could ...file is open for anyone to use.  View the Addgene Plasmid Request Data Dashboard! Figure 1: The ...and the source code is available in the Github repository (you can find the link in the upper right hand...

  19. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...of the new plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles.  ...2 nucleocapsid protein (Plasmid #141391-LV [+N]) or membrane protein (Plasmid #141386-LV [+M], negative... over the original CROPseq. Find CROPseq-multi plasmids here Walton, R.T., Qin, Y., Blainey, P.C. (2024...potential improvements to the editor. Find PE7 plasmids here!     Figure 2: PE7 improves prime...antibodies were recently added to the Addgene repository of tools for SAR-CoV-2 research. Two new antibodies...conjugated with its HaloTag domain.  Find Voltron2 plasmids here! Abdelfattah, A.S., et al. (2023) Neuron...therapeutic applications.  Find microglia-targeting AAV plasmids here! Serrano, C., et al. (2023). Simple and ...

  20. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    Published
    April 2, 2019, 1:44 p.m.
    ... to see for yourself. We have one plasmid in our repository, plasmid 39624, that contains cmr.   tinman...stories behind the names. We also have many plasmids in our repository that you can use to study these genes... 1998). Interested in plasmids that contain the groucho gene? Check out plasmids deposited by the Shivdasani... several plasmids that contain SHH, you can find them all here! The most popular SHH plasmid however was...website for plasmids that contain tinman or the mammalian homolog Nkx2.5. We also have one plasmid, Addgene...Drosophila Read our Plasmids 101 blog series Resources on Addgene.org Find plasmids for your research ... Beauty (Ivics et al., 1997). We have a lot of plasmids that contain components of the SB transposon system...

  21. AAV Packaged on Request is Here!

    Type
    Blog Post
    Published
    Jan. 10, 2025, 1:01 p.m.
    ... of over 3,000 eligible AAV cargo plasmids in the Addgene repository to be packaged in your choice of ...Request With Packaged on Request, you can find your plasmids and your viral packaging service in the same place...packaged on request process, simply navigate to your plasmid of interest. If it’s eligible for packaged on request...special processes involved; simply request your plasmid of interest in your desired serotype, place your...

  22. Addgene at SfN23!

    Type
    Blog Post
    Published
    Nov. 9, 2023, 2:15 p.m.
    ...curation manager, Angela, experts on the Addgene repository, are also attending! Finally, our team is rounded...ready-to-use antibodies, viral vectors, and, of course, plasmids! Plus, we'll be giving away fun Addgene swag for...

  23. Fueled by Coffee at #SfN14

    Type
    Blog Post
    Published
    Nov. 18, 2014, 7:15 p.m.
    ...exhibitor booth in order to help scientists find the plasmids they need for their research, show labs how they...benefit from depositing their constructs to the repository, and getting feedback from our community. It ...about all the amazing research being done with plasmids. I heard an impressive talk about the use of optogenetics...and resources by storing and distributing their plasmids with the scientific community. All of this speaks... meet our Addgenie crew and sign-up for a free plasmid! Also check out the PLOS Neuroscience Community...

  24. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    Published
    Oct. 17, 2024, 1:15 p.m.
    ...here at Addgene — your contributions make the repository what it is. So we’re thrilled this week to congratulate...evolution. The Baker Lab has deposited multiple Top7 plasmids at Addgene, along with other tools like high resolution...

  26. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ...service features 210 plasmids: empty backbones, fluorescent protein tags, SARS-CoV-2 plasmids, etc. It is our...to Elute and Store Your Plasmid DNA? by Paolo Colombi Should I store my plasmid in TE, Tris, or water?...Here we cover five methods to do this. 5. Plasmids 101: Plasmid Incompatibility by Leah Schiesow If you're... We've had 11 blog posts on COVID-19 featuring plasmid tools from depositors who quickly shared their ...expression, and more. We also highlighted reagent repositories and other collaborative efforts in the scientific...you're thinking about hosting multiple plasmids in one cell, it might be that you can't stably maintain...This blog post features two mechanisms that cause plasmids to be incompatible. 4. RNA Extraction Without...

  27. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    ...feature some recently deposited plasmids every quarter. The “hot plasmids” from last March were quite popular...behalf of scientists. 9. Plasmids 101: Positive and Negative Selection for Plasmid Cloning by Jennifer Tsang...Addgene Browse the repository Find educational resources at Addgene Search for plasmids at Addgene ...past year: CRISPR, optogenetics, chemogenetics, plasmids, fluorescent proteins, careers, and more. These...s the top 10 blog posts from the year. 10. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent... about mNeonGreen, mTFP1, and mWasabi and find plasmids containing these fluorescent proteins at Addgene..., and all possible base-to-base changes.   1. Plasmids 101: Transformation, Transduction, Bacterial Conjugation...

  28. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    Published
    Feb. 27, 2020, 2:15 p.m.
    ...of information about how to deposit plasmids, store your plasmids or viral items, technical support, and...your favorite store is easy. Ordering genes on plasmids online is a little trickier -- but at Addgene,...nonprofit, Addgene does not own the material in our repository. We distribute it on behalf of depositing institutions...

  29. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These brief articles... 50,000 plasmids, we can't write posts for every great plasmid that comes into the repository, but be ...articles provide a synopsis of a plasmid or group of plasmids' functions and applications. We hope that... you to find and use the plasmids you need. You can find all the hot plasmids from 2017 below. With over...write about your plasmids in a future blog post. No time to read? Listen to our hot plasmids segment on the...editing for precise genome modification. Plasmid ID Plasmid Name Cas9 Variant Speacial Features 85169... to the Addgene blog to get all the latest hot plasmid updates!     Tol2 Gateway-compatiable toolbox...

  30. New Viral Vectors - Spring 2025

    Type
    Blog Post
    Published
    April 29, 2025, 1:30 p.m.
    ...New ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor  Notes ...service, which offers over 3,000 eligible transfer plasmids and five different serotypes. Not sure what to...

  31. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

    Type
    Blog Post
    Published
    Feb. 1, 2018, 3:07 p.m.
    ...sequencing results for plasmids in the repository at a glance. Our Snapgene-powered plasmid maps and sequences... we use SnapGene for plasmid quality control are: To identify important plasmid features To align NGS...design experiments for further plasmid verification Identifying plasmid features While our quality control...control process for incoming plasmids can vary based on the contents of a plasmid, the first step is always...send the plasmid off for Sanger sequencing.     If a repetitive region is present in the plasmid, we might...the Addgene Blog How to verify your plasmid Check out our Plasmids 101 featured topic page Additioinal...results to perform quality control on deposited plasmids. We’ve also talked about how our new Snapgene ...

  32. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    Published
    Nov. 14, 2016, 3:30 p.m.
    ... Requested Plasmid in 2015 Find Hot Plasmid Technologies Write about Your Favorite Plasmid Technology ...around the world choose to share their plasmids through our repository since we know biological resource centers... indicates that a plasmid has been requested 20+ times   A red flame means a plasmid has been requested...answering plasmid requests can be a time-consuming chore for labs that have popular plasmids but lack ...All plasmids deposited at Addgene enhance scientific sharing worldwide but a select few are particularly...particularly special. Over 1000 of our 60,000+ deposited plasmids have been requested more than 100 times each, ...to sharing epitomized by the creators of these plasmids with the first annual Blue Flame Awards. What'...

  33. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Published
    July 3, 2014, 3:48 p.m.
    ... the successful, internationally renowned plasmid repository that it is today. Although I wish I could...music playing all night was hand-picked (like our plasmid samples!) by Addgenies. If you were there, you ...Addgene uses E. coli bacteria to generate copies of plasmids. The community of scientists, part of the Addgene... complicated process of requesting and sharing plasmids has become simplified through Addgene. Looking...Check out our impressive stats from 10 Years of Plasmid Sharing Or read more about what happens Inside ...

  34. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    Published
    June 27, 2018, 1:16 p.m.
    ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...write about a recent plasmid deposit please sign up here. Listen to the Hot Plasmids Podcast!  MarathonRT...’s lab at Yale University recently deposited a plasmid for expressing a novel reverse transcriptase (RT...polymerase processivity. Please find the MarathonRT plasmid here! Zhao C, et al. RNA. 2018 PubMed PMID:29109157... cell surface. Their toolkit includes a set of plasmids each containing an anti-GFP nanobody fused with...fluorophores. Find all of the nanobody toolkit plasmids here. Buser DP, et. al. PNAS 2018. PubMed PMID...

  35. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...of the new plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. ...Think we missed something hot? You can pitch a Hot Plasmids section (antibodies and viral preps welcome!) ... you can also find several other constructs in plasmid form. Fig. 1: Summary of the approach and...JEDI-2p viral preps here (and here!)Find JEDI-2p plasmids here!  Liu, Zhuohe et al. Sustained deep-tissue... improve the performance of HcKCRs. Find HcKCR plasmids here!   Govorunova EG, et al. Kalium channelrhodopsins...’ in the Addgene catalog pulled up over 13,000 plasmids! Our recombinant anti-c-Myc [9E10] (isotype IgG2a...myc-tagged protein GFP-smFP-myc expressed from Plasmid 98926. See the Applications section on the Anti-c-Myc...

  36. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

    Type
    Blog Post
    Published
    July 18, 2017, 12:41 p.m.
    ...Addgene-Seeding Labs plasmid grant is a collaborative endeavor providing plasmids to researchers in developing...Addgene-Seeding Labs Plasmid Grant Check Out Previous Addgene-Seeding Labs Plasmid Grant Awardees Listen...scientists who were awarded the Addgene-Seeding Labs Plasmid grant this year. These scientists work on projects...as the pET GFP LIC cloning vector and the pOKD plasmids. The recombinant Cry proteins will then be screened...HyPer3 (2) which he will co-express together with a plasmid encoding glycine oxidase. The goal is to determine... develop technologies to protect fresh water repositories in Mexico. At Addgene we're happy to be able...excited to see how all of the Addgene-Seeding Labs Plasmid Grant awardees' projects progress. Subscribe to...

  37. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    Published
    Dec. 5, 2019, 1:49 p.m.
    ...look clean and easy to use.  Easily search for plasmids (and more!) The main goal for the homepage was...functionality. We wanted users to be able to find plasmids and viral vector tools more easily. In the redesign...unique: community, sharing, and fun. The Addgene repository is built on the foundation of a community that...hope every time you come to Addgene to find the plasmid or viral vector for your next experiment, that ...

  38. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type
    Blog Post
    Published
    Nov. 20, 2018, 8:17 p.m.
    ...materials that we use in our labs, including plasmids. All plasmids at Addgene have unique ID numbers associated...identify a plasmid available through Addgene and find verified information about each plasmid item. Given...associated plasmid page on the Addgene website and find specific information about the plasmid: name, description...description, how the plasmid was generated, validated sequence information, and more. The plasmid page also helps...publications that have used the same plasmid through the “Articles Citing this Plasmid” section. Having IDs linked...specific plasmids used. RRIDs and resolvable compact identifiers now exist for all Addgene plasmids! In an...our example plasmid, lenti-Cas9-VQR-Blast. If you scroll down to the bottom of this plasmid’s page you will...

  39. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...you find what you need.   More Plasmid Resources from Addgene: Plasmids 101 blog series Addgene's Molecular... the most frequently requested vectors in our repository and will discuss some of the features you may...need. You will also have to make sure that your plasmid has been incorporated into the host organism, usually...open reading frame (ORF) of interest –  try pRS plasmid shuttle vectors. You may prefer the more traditional...details and guidance for choosing your perfect plasmid backbone. Or just ask –  leave a message in the...Molecular Cloning Guide & Other Plasmid Protocols List of common epitope tags ...

  40. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...about Popular Plasmids in the Repository Learn about New Plasmid Technologies in our Hot Plasmid Articles ...to create a plasmid based on the biotin ligase BirA backbone (Eric Campeau, Addgene plasmid #26624) was...inserting BirA into a pMal plasmid (NEB) thus creating the pMal-T-Avi-His/BirA plasmid now available through...other Hot Plasmids Find Additional Posts on Mol Bio Techniques Find Additional Posts on Plasmid Technologies...controlled easier than formation of GST dimers. Avi-Tag plasmid for improved biotinylation selectivity A common...exploit the best of both worlds. We generated a plasmid that produces biotin ligase and a protein of interest...and a 6X HisTag for affinity purification (see plasmid map and cartoon below). Including biotin in your...
Showing: 41 - 80 of 148 results