960 results

Learn about the most common injection routes in mice, including their key differences and uses.

Jan. 22, 2025, 9:42 p.m.

Making the transition from bench to management can be daunting, but these tips can help!

Jan. 21, 2025, 2:15 p.m.

Adeno-associated virus (AAV) vector serotypes can help you target specific cell types through their tissue tropism.

Jan. 16, 2025, 2:15 p.m.

Prime editing is a CRISPR/Cas9 editing method that offers more versatility and precision to genome editing. This post covers common prime editing tools.

Jan. 13, 2025, 9:49 p.m.

Addgene's AAV Packaged on Request option has officially launched! Learn more about how this expansion of our helpful viral vectors service can help your research.

Jan. 10, 2025, 1:01 p.m.

This Twelve Days of Antiboides, we're celebrating some cool new additions to our antibody repository and resources.

Jan. 6, 2025, 5:17 p.m.

Twenty years have gone by fast! Join us to share some highlights of Addgene and our community as we close out our 20th anniversary celebration.

Dec. 19, 2024, 2:15 p.m.

A round up of guest blog posts of 2024. Thank you to our guest contributors!

Nov. 26, 2024, 2:15 p.m.

Learn how Addgene has worked to increase accessibility to our materials and educational resources.

Nov. 21, 2024, 10:47 p.m.

Check out the latest Hot Plasmids, viral vectors, and antibodies in the Addgene repository!

Nov. 21, 2024, 1:30 p.m.

Addgene has a new animation showing the common ways antibodies are used to study proteins in the lab!

Nov. 19, 2024, 2:15 p.m.

Did you know CRISPR can be used to target non-coding RNAs? In this blog post, we explain the pros and cons of different approaches.

Nov. 14, 2024, 2:15 p.m.

Learn about the differences in virus production between insect cells and mammalian cells.

Nov. 5, 2024, 8:32 p.m.

Antibody data repositories and search engines can help you find the right antibody for your experiment. Check out a list of helpful links to get you started.

Oct. 31, 2024, 1:15 p.m.

Introducing the Antibodies 101 eBook, the latest addition to Addgene's helpful eBook collection!

Oct. 29, 2024, 1:15 p.m.

Congratulations to Addgene depositor David Baker, who was awarded the Nobel Prize in Chemistry last week alongside Demis Hassabis and John Jumper!

Oct. 17, 2024, 1:15 p.m.

Starting in January 2025, Addgene's Viral Vector service will include a Packaged on Request option.

Oct. 15, 2024, 1:15 p.m.

Addgene has updated our useful CRISPR guide to include PRIME editing, new enzymes, and more!

Oct. 10, 2024, 1:15 p.m.

Where did plasmids come from? Learn more about natural plasmids, the benefits they offer cells, and how scientists have adapted them into laboratory tools.

Oct. 8, 2024, 1:15 p.m.

Addgene adds new ready-to-use viral vectors to our service every month. Read all about the latest additions here!

Oct. 3, 2024, 1:15 p.m.

Sequence and ligation independent cloning (SLIC) lacks the strict requirements of many standard cloning techniques. Learn how to use SLIC in this post.

Sept. 26, 2024, 1:15 p.m.

Magnetoresponsive proteins have seemed like a dream... but Andrew York's lab has developed and deposited one, opening the possibility of magnetoresponsive tools!

Sept. 24, 2024, 1:15 p.m.

Addgene's excited to launch a new series of YouTube videos: Quick Tips!

Sept. 19, 2024, 3 p.m.

A dive into troubleshooting and optimization steps you can take to improve your western blot or identify and fix issues within the assay.

Sept. 17, 2024, 1:15 p.m.

Addgene's Antibody Data Hub can help you select the antibodies for your application.

Sept. 12, 2024, 1:15 p.m.

If you can't find a validated antibody for your flow experiment, this blog post can help you validate your antibodies yourself.

Sept. 10, 2024, 1:15 p.m.

There are a few different types of Fab fragments to consider when you need a smaller affinity reagent than antibodies. Learn about them here!

Sept. 5, 2024, 1:15 p.m.

Fragmid is a quick, easy way to design and create CRISPR plasmids. Read about the Doench lab's new modular cloning-based approach in this blog post!

Aug. 29, 2024, 1:15 p.m.

Check out some of the new and useful tools recently deposited in the Addgene repository!

Aug. 27, 2024, 1:15 p.m.

Western blots have many technical elements. We cover each element, and the different options available to choose from, in this post.

Aug. 22, 2024, 1:15 p.m.

If your flow plot has a smear instead of distinct populations, you can still intepret the data! In this post, we discuss how to interpret plots of all kinds.

Aug. 20, 2024, 1:15 p.m.

In this blog post, we walk you through how to create and use a serial dilution in your experiments.

Aug. 15, 2024, 1:15 p.m.

Addgene's antibody lab member Kate describes each step in recombinant antibody production process.

Aug. 13, 2024, 1:15 p.m.

Curious about what it's like to be a summer intern at Addgenie? Addgenie Intern Toj explains it all!

Aug. 8, 2024, 7:22 p.m.

In this blog post, we explain gamma-retrovirus packaging systems for those interested in using gamma-retroviral systems in the lab.

Aug. 6, 2024, 1:15 p.m.

This post explains how to strip and reprobe a western blot, which is useful for detecting multiple targets or for correcting errors.

Aug. 1, 2024, 1:15 p.m.

Addgene's viral vector service has grown much since it was first launched in 2016! Read about our service and the unique challenges it presents in this blog post.

July 30, 2024, 1:15 p.m.

Which ELISA is right for your experiment? Read our blog post to help you decide!

July 25, 2024, 1:15 p.m.

An overview of Addgene's MTA process.

July 23, 2024, 2:17 p.m.

Ready to produce rAAV in your own lab? This 101 post can help!

July 16, 2024, 1:15 p.m.

The latest edition of Addgene's Viral Vectors 101 eBook is here!

July 11, 2024, 1:15 p.m.

Addgene adds new ready-to-use viral vectors to our service every month. In this post, we share 20 new viral preps we've added in the last quarter.

July 9, 2024, 1:15 p.m.

Learn about CRISPR terminology for common DNA, proteins, and RNAs involved in this CRISPR 101 blog post.

July 2, 2024, 1:15 p.m.

Learn about flow cytometry labeling strategies beyond extracellular markers in this Antibodies 101 post!

June 27, 2024, 1:15 p.m.

PRIDICT 2.0 is an updated software that can help you design gRNAs for PRIME editing. Read more in our guest post!

June 25, 2024, 1:15 p.m.

Plasmids can be under either stringent regulation of replication or relaxed regulation of replication. In this blog post, we explain how stringent regulation works.

June 20, 2024, 1:15 p.m.

Addgenies came together to celebrate our 20th anniversary!

June 11, 2024, 1:15 p.m.

Flow cytometry can require some extra controls, described in this blog post!

June 6, 2024, 1:50 p.m.

Curious about how, exactly, Cas9 does what it does? Dive into the structural biology of Cas9 for an answer!

June 4, 2024, 1:15 p.m.

Once you create a pooled library, you'll need to ensure it has an amplification protocol. Read our blog on how to develop a protocol for your library!

May 30, 2024, 1:30 p.m.