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We narrowed to 610 results for: STO;

Showing: 51 - 100 of 610 results

  1. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ... it out. 4:00 PM--Respond to customer help tickets. Catch up on customer help tickets, and respond when...advocate for the customer and help build software and technology tools with the customer in mind. You can...Oxford Nanopore Technologies, I work with our customers in the US to learn where the gaps are in their... so many different people! I communicate with customers, software developers, as well as team members ... It’s divided into the key takeaway themes and customer pain points we’ve identified. I can’t wait to ...know what to work on next to help alleviate any customer pain points. Many thanks to our guest blogger...

  2. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...usual quality and success. Customer and technical support Our customer and technical service brands...helpful and the customer and technical service teams put 100% effort into helping our customers every day in...have for the future. At Addgene we will never ever stop planning, perfecting, and learning, but there are...scientific fields.” Thanks to our legal team, and great customer service, somehow we have scientists actually loving...offer online for Addgenies and to serve external customers. I also can’t wait to see where our new data initiatives...to do it quickly. Hiring is hard but as a 3 year Boston Globe and BBJ Best Place to Work with a positive...

  3. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type
    Blog Post
    Published
    Feb. 2, 2018, 3:20 p.m.
    ... to learn things quickly. Story telling. The ability to know what’s a story and how to hook your readers...feels that storytelling is an impactful tool for science communicators. “The power of a story is to make...there’s always plenty of writing: plain-language stories about Fred Hutch research, pieces for donors such...that can be a very painful process. And certain stories can make you feel like ‘I’m a terrible writer. ...through connections and that’s something that good storytellers in science can do.” Susan’s number one piece...social media as a scientist here Learn more about storytelling in science ...

  4. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...presence of a stop cassette upstream of the target gene in the targeting vector. The stop cassette is floxed...embryonic stem cells that are then injected into a blastocyst. There are several types of genetically engineered...Modified embryonic stem cells are injected into a blastocyst which is then implanted into a female animal ...embryonic stem cells, which are then injected into a blastocyst. Nucleases can also be used to engineer knockout...create mosaic animals where not all cells in the blastocyst were successfully edited by the injection of ... variations of this technology can be used to customize the effects of this recombination. To generate...floxed, so Cre-mediated recombination deletes the stop cassette, which permits expression of the target...

  5. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ...bring out these interesting stories and remind the panelists of the stories or topics you know they can... taking good notes and discovering interesting stories, riffs or opinions that each panelist can contribute...identify the funny, provocative or interesting stories that will make for a fun discussion. People are...they can see each other) on comfortable chairs (stools if you have to, but this is tough for women in ...especially short people – which I am, so I hate stools). I like it when the moderator sits in the middle...question in a way that elicits those interesting stories and opinions. Don't let all the panelists answer...moderator's job to politely but firmly get them to stop talking and redirect the conversation. The person...

  6. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...production is Komagataella phaffii (a.k.a  Pichia pastoris) because it secretes fewer of its own proteins...applications for validation include western blot, immunohistochemistry, and immunocytochemistry. It’s important...applications. An antibody that performs well in immunohistochemistry may not perform well in a western blot. ... to their parental hybridomas. Aggregation and storage Aggregation of antibodies may impact their quality...testing. An aliquot of our recombinant antibodies is stored at 37 °C for a period of two weeks. After this ...that are not equally effective are shipped to customers on ice, preventing any temperature shifts. Of ...we are also cautious during the aliquoting and storage process to prevent dropped tubes. Now that your...

  7. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...primary role is customer support, but there are eight other teams who still retain a customer support element...when I discovered that many of my old suitemates stopped talking to each other if I was not there to arbitrate...to listen, and an ability to remember the right stories at the right time. Like many of the posts in our...because confirming that every person has read and understood every communication that gets sent out through...other responsibilities. Knowing which aspects of customer support each team “owns” is critical for day-to-day...-to-day operations and keeping our customers happy, but inevitably there will be gray areas that either...another job, it will be important to retain the stories and examples that have guided your resolution processes...

  8. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...either side of a stop cassette, upstream of an effector gene. In cells where the stop cassette is removed...of cells will express the effector or retain the stop cassette following recombination. Image from Isaacman-Beck...step, the effector is expressed. In cells where the stop cassette remains, the effector is not expressed ...competing attP sites to drive expression of either a stop cassette or an effector gene. HDVR (2X Hepatitis...attP site. Truncating the attP site upstream of the stop cassette results in decreased efficiency of recombination...preferentially leads to gene constructs where the stop cassette remains following recombination. This strategy...

  9. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

    Type
    Blog Post
    Published
    July 2, 2015, 8:37 p.m.
    ...would love to tell such a dramatic story, but it wouldn’t be my story. I love academics. If I didn’t, it...there was even a time when I was too nervous to ask store clerks for help at the mall. In undergrad I was ...to open up a little after I dropped myself into Boston University with none of my old friends to fall ...thought that I would be best poised to share the stories and difficulties behind great discoveries by tackling...actually finishing up this post on a plane back to Boston from Kentucky where I spent a couple of days talking... mission to make research easier. I’m hoping my story demonstrates that transitioning from academia doesn...

  10. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...fresh stocks. Most antibiotics are stable in powder form, but quickly breakdown in solution. Storing aliquots...them to media hotter than about 55oC and store plates/stocks wrapped in foil if a light-sensitive antibiotic...tool in molecular biology, yet scientists rarely stop to think about how much easier it makes our lives.... For instructions on how to prepare antibiotic stocks, see Addgene's Reference Page. How else can antibiotics...antibiotics be used in the lab? Historically, antibiotics have also been used to disrupt genes at the chromosomal...

  11. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...their CPP system to study protein transduction, D’Astolfo et al. made the surprising discovery that both...NDSB-201) were required for protein delivery. D’Astolfo et al. coined the term iTOP (induced transduction...that iTOP functions through macropinocytosis. D’Astolfo et al. tested iTOP in a series of primary cell...resulting in GFP expression. Image courtesy of Diego D'Astolfo. CRISPR/Cas9: a potential application of iTOP...Having tested iTOP using Cre-lox recombination, D’Astolfo et al. were eager to see if iTOP would be compatible...cleave the specified target. Figure adapted from D’Astolfo et al. In certain instances, the potential...Efficient Intracellular Delivery of Native Proteins. D'Astolfo DS, Pagliero RJ, Pras A, Karthaus WR, Clevers ...

  12. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    Published
    July 13, 2017, 2:30 p.m.
    ... maintain many fly stocks that are available to everyone. Flybase lists what stocks are available at Bloomington...start of the day and throughout daytime. They mostly stop emerging at night. An incubator with a light cycle...genetic crosses. More importantly, this knowledge will stop you from embarking on a fruitless journey of trying...information about gene function, while others offer fly stocks and other reagents. If you’re ever confused about...where the gene is expressed within the fly, whether stocks of mutant flies exist, whether any phenotypes have...mentioned the gene. The Bloomington Drosophila Stock Center at Indiana University is another tremendous...

  13. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...anniversary celebration, we’ve been finding and sharing stories unique to our repository and resources. As editor...picked our viral vector service as one of those stories. Viral vectors are viruses engineered to be safely...available and number of requests tell only part of the story. Viral vectors are, after all, a delivery method...blog, you may recall our first 20th anniversary story on CRISPR, where we dove fairly deeply into the ...primary literature, soliciting information from requestors and depositors, and identifying trends in our...actually show you if the vector was useful for the requestors or how it performed. Addgene launched our AAV...platform for the sharing of that exact data. Here, requestors can upload their experimental data in a standardized...

  14. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...norvegicus genes! Mexican axolotl (Ambystoma mexicanum) Ambystoma mexicanum, aka the axolotl. Image...are five more model organisms that have left a historical impact on the scientific community. (And, check...  The E. coli genome has an extensive research history and characterization. Many proteins and processes...organisms in the world. With its extensive scientific history, highly characterized genome, and widespread utilization...organisms is its thoroughly developed and defined history in research (Krämer, 2015). As a result, a vast... https://doi.org/10.1159/000490898 Gibbs, R., Weinstock, G., Metzker, M. et al. Genome sequence of the...

  15. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...copies of a tRNA that specifically recognizes amber stop codons (TGA) to encode phosphoserine, the Sep-tRNA... the phosphorserine OTS recognizes the TAG amber stop codon, using the OTS in an unmodified bacterial ...would result in read-through translation of many stop codons and detrimental effects on cell growth. The...was genomically recoded to eliminate all 321 amber stop codons (TAG), replacing them with TAA (7). This ...TAG codon should encode phosphoserine instead of a stop codon, which will allow full length GFP to be translated...can also be cloned with an ORF of interest for customized experiments. Once the ORF of interest is cloned...suppression, as described (6). To determine the stoichiometry of phospho-incorporated to non-phospho-incorporated...

  16. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...negative” data We all want to be able to tell a good story when presenting scientific findings. This makes ...of information and, from a SciComm perspective, stories with links of causation from initial hypothesis...validation are easier to understand. The need for great stories has, however, made it difficult to publish so-called...original hypotheses. Looking for novelty in our stories, we are additionally averse to producing papers...published results. This over-reliance on the perfect story for a “high impact” publication can lead to the ...the tested hypothesis is correct (a great success story) even though the majority of the unpublished evidence...generate can, however, bias us. We’ve all heard stories and read papers wherein a researcher tries 5 separate...

  17. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...individuals through stock or investor pay outs. Addgene has no investors or stock. All of our proceeds...processes, distribution, and customer service) Addgene provides unmatched customer service that is consistently...

  18. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    Published
    Nov. 29, 2016, 3:30 p.m.
    ... chose the stop motion format for our videos as it is the most approachable for LEGOs. Stop motion consists...pictures together to form the full movement. Producing stop motion videos is very time consuming; producing ...toys” gaining life and working as scientists in a custom made, colorful laboratory. When we started, the...child to work. If you have an odd or quirky lab story you’d like to see portrayed in LEGO form, feel free...us at [email protected]. Who knows, your story might just get “legofied.” Many thanks to our guest...

  19. Advice for Choosing a Research Project

    Type
    Blog Post
    Published
    April 17, 2014, 2:45 p.m.
    ...research project must have innovative yet attainable milestones that contribute to intellectual thought and discovery... The research project also serves as a stepping stone into the next professional stage of your career ... and skills do you need to use to reach these milestones? Can you get assistance from people who have ...for guidance, intelligent discussion, and/or brainstorming sessions to maximize productivity and direction...Think about what your personal goals are and set milestones along the way so that you don't get stuck doing... Passion fuels the drive to continue persuing milestones and working hard. Being patient, being selective...

  20. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    Published
    Jan. 24, 2019, 2:24 p.m.
    ...Leiwe and Takeshi Imai from Kyushu University. Stochastic multicolor labeling is a powerful solution for...microscopy-based neuronal reconstruction. To achieve stochastic multicolor labeling, Brainbow used the Cre-loxP...copies of the transgene cassette are introduced, stochasticity will result in a combinatorial expression of...Brainbow was inherently low. This is because the stochastic and combinatorial expression of fluorescent proteins... such vector-based gene delivery systems, the stochastic expression of XFPs is possible by simply introducing...introducing a mixture of XFP genes. Stochastic multicolor labeling is possible when each of the XFP genes...

  21. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...exciting story I can tell with my data?  Can I make better slides that more effectively tell a story? Generally...publications (published, not in preparation) A history of successful funding (fellowship grants) A project...additional controls necessary? As you build your story, are additional data needed to rule out alternative...Before you do your first experiment in your new lab, stop and plan it out. Some questions to answer include...presentation. Yet, PFs need to tell and sell their stories to get jobs. PFs (and mentors) can never be too...question is yes. As you get better at telling your story, you can find clearer ways to consolidate or present...

  22. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...widely varied with many properties, having well-understood standardized terms makes it a lot easier for ...plasmids at Addgene are shared under, is well-understood and accepted by the majority of research institutes...Recipient (in Addgene parlance, the depositor and requestor) organizations. Over the last twenty years, we...your organization agrees to let Addgene receive, store, and distribute your material under the specified...move forward with shipping the material to the requestor.  Why the MTA?  The MTA process can be a bit confusing...exceptionally fast tech transfer offices with the Golden Stopwatch award and seek feedback as to how we can make ...

  23. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...offerings! We recently re-amplified our distribution stock of the Brunello Human gRNA library, and we thought... a file in FASTQ format, a standard format for storing NGS reads and their corresponding sequencing quality...in an automated manner. Addgene commonly uses a custom script derived from the count_spacers.py file from...representation of your library. When plotted as a histogram, the counts will give you a bell curve of the ...finds that this representation is most easily understood visually. As an example, the curves from our ...screening, and if you have any tips, input, or stories of interest about library use, let us know in the...

  24. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    Published
    July 10, 2014, 7:17 p.m.
    ...help win a business plan competition and advance investors to the next stage of diligence." In business competitions...non-scientific audiences. "There are 100 ways to tell a story - convince your audience you are doing amazing things...The team is key, and the initial team is the cornerstone of it all. Without a good nucleus, the company...are 100X better than current technology and get customers to change behavior. When you are ready to commit...crowd funding, federal funding, foundations, and investors are examples of various sources of funding. Always... Biology & the Importance of Assembly Standards Story of a SynBio Startup: RevBio's Epiphany (or Lack ...

  25. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...privilege to find and share what stories we can, and to hear the stories others have to tell.  "Also thanks...we wanted to walk through some of our favorite milestones and share some thoughts from the community that...six years to reach our first major repository milestone — 10,000 plasmids deposited and available — and... which was a major barrier for depositors and requestors alike. We transformed this process by aligning...Addgene reached the 500,000 plasmids distributed milestone! We were thrilled to see the impact our repository...

  26. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ... Outlines a detailed scope of specific aims, milestones, possible setbacks, and potential alternative...Carefully read over all parts until all aspects are understood. While reading the funding description, pay attention...will address these limitations and lead to new milestones in the field. Research strategy The Research...aims The Specific Aims section highlights each milestone that must be met in order to deem your proposal...proposals consist of 3 to 5 aims, with secondary milestones to meet along the way to achieving each aim. ...comments may make it clear that the reviewer misunderstood the point that was being made, take it as an...

  27. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    .... The viral genome DNA is further protected by histone-like proteins forming the nucleocapsid. B) Three...recommended to test for the presence of RCA in the viral stock by doing a plaque formation assay on A549 cells ...recombination for amplification. The final purified plasmid stock should be analyzed by endonuclease restriction digest...you’ll produce the primary recombinant adenoviral stock (rAdV-S). HEK293 cells are transfected with the ...lysate is your primary low titer rAdV-S, and can be stored at -80C for later use or used immediately for amplification.... Purified high-titer rAdV is then dialyzed and stored at -80C. Purification and concentration kits that...

  28. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...advocating-for-science-boston-2016/. Sign Up For the Advocating for Science Symposium Boston 2016 Changes since...Science Symposium and Workshop 2016 – Sept 16-17 MIT, Boston The Advocating for Science Symposium and Workshop...also started progress towards solutions. The 2015 Boston FoR meeting was designed to explore areas where...various levels, with panelists including: Kate Stoll, PhD - Senior Policy Advisor MIT Washington Office...Professor of Sociology. University of Massachusetts Boston Christine Oslowski, PhD - Biotechnology Communications...

  29. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...cause bad reactions in mice. Customization AAV offers a level of customization that is unmatched by other...over the past decade. CRISPR screens are easily customizable and enable the targeting of hundreds to tens...lentivirus but offer an improved safety profile, customization, and enhanced expression in many mouse tissues...combination of serotypes and psuedotypes means you can customize your CRISPR screen to a high degree. This is particularly...interrogate immune cells to improve immunotherapy in glioblastoma, and generate programmable screening platforms...

  30. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    Published
    July 7, 2020, 1:15 p.m.
    ...Harris Lab relies on the restoration of a frame-shift in mCherry that restores fluorescence to monitor ...that disrupts fluorescence due to a frameshift. Restoration of fluorescence can only occur through editing...results in two DSBs that can be repaired by NHEJ restoring mCherry expression. Cells can then be sorted using...C-to-T editing by the APOBEC-Cas9 editosome would restore eGFP fluorescence, making it easy to quantify single... point mutations in eGFP that results in the restoration of fluorescence. Transient reporter for editing...

  31. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...proximity labeling and TransitID Tracking cellular history with protein ticker tape Teaching an old antibody...on-target delivery of genetic cargo  By Alyssa NeuhausTo improve delivery and reduce off-target effects....1101/2023.02.07.527548.    Tracking cellular history with protein ticker tape By Brian O’Neill The Cohen...created a system for recording the transcriptional history of a cell. Lin et al. generated a fusion protein...ticker tape images could recover transcriptional histories, accurate to within 40 minutes. Figure ...their initial tests with thick slice (70 μm) immunohistochemistry (IHC) (Figure 5). PSD-95 is a scaffolding...

  32. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...control by Addgene before being distributed to customers. Details about our production protocols, titering...vivo studies. Preparations are then aliquoted and stored at -80 °C. Titer Titering is either performed by...preparations is assayed by comparing the relative stoichiometric ratios of the viral capsid proteins VP1, VP2...The preparations are then aliquoted, frozen, and stored at -80 °C. Concentrated lentiviral preparations...then resuspended in PBS, aliquoted, frozen, and stored at -80 °C. Titer All titering is performed on lentiviral... lentiviral preparations that have been stored at -80 °C and thawed. This ensures that any loss of titer...

  33. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...structures (at < 5 Å resolution) of the linker histone H1.8 in Xenopus nucleosomes and in complex with...has developed a new epigenetic editor, Coupled Histone tail for Autoinhibition Release of Methyltransferase...can be toxic, CHARM uses a cofactor fused to a histone tail to recruit and stimulate the endogenous methyltransferase...uses a fusion of the DNMT3L cofactor (D3L) with a histone tail (H3K4me0) to a DNA-binding protein (dCas9 ...team to develop self-silencing CHARM variants that stop their own expression after silencing their target... a familiar challenge for anyone who has done histology on brain tissue is identifying the cells you are...

  34. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    Published
    Feb. 27, 2020, 2:15 p.m.
    .... We are the Customer Support Specialists here at Addgene. If you’ve called our customer service line ...'Regan Ordering jeans online from your favorite store is easy. Ordering genes on plasmids online is a ...really getting at one bigger question: How does a customer place an Addgene order using a PO number? Why ...full of information about how to deposit plasmids, store your plasmids or viral items, technical support,...

  35. Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton

    Type
    Blog Post
    Published
    July 7, 2017, 1:32 p.m.
    ... Center for Regenerative Medicine of Boston University and Boston Medical Center. Special thanks to our...director of the Center for Regenerative Medicine at Boston University Medical School. Darrell’s research focuses...Chapter 1: Introduction to Darrell Kotton and the Boston University Center for Regenerative Medicine. Darrell...Center, and the Pulmonary outpatient clinic, all at Boston Medical Center. In addition he conducts basic stem...

  36. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...resistance gene (missing the first two codons). Two stop codons are inserted between the truncated gene and...ligated into the MCS/SacI site in pUCXKT, removing the stop codons during backbone digestion and providing the...Toolkit for constructing fusion proteins with customizable N- and C-terminal modules   Thanks to the lab...Technologies. The MultiSite Gateway Kit is a customizable Gateway®-based toolkit that allows scientists...bound to the mRNA since they are tethered AFTER the stop codon. Thus a non-translated mRNA will appear yellow...: 10.1126/science.aaa3380 MuLE vectors for customized lentivirus production Finding a lentiviral vector...LacZ, puromycin or CreERT2 can be combined with custom-cloned vectors in a Multisite Gateway reaction ...

  37. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...my training, I dove straight into my capstone project. A capstone project is a major project given to ...Figure 1: Addgene's summer interns presenting their capstone projects during the Summer Intern Poster Presentation... scientist. But I expected something wrong. My capstone project was based on plasmid cloning and I, the... Once everything was confirmed, I worked on my capstone project report and presentation, during the final...

  38. 10 Ways to Share Your Science!

    Type
    Blog Post
    Published
    May 13, 2014, 8:34 p.m.
    ...you questions. Remember that people like a good story and science involves characters – in this case you...or other places online, and consider how those stories are being told. Twitter can be a good way to follow...surprised to find that his friends and family finally understood why he cared to study mice on some distant island...the East Coast, there’s programs at both MIT and Boston University. At Addgene we're committed to scientific...sharing – both your plasmids and your research stories. Consider practicing communicating your research...

  39. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    Published
    June 28, 2018, 3:01 p.m.
    ...IP and ChIP assays. Tag 96 genes at a time using Custom Synthetic cr:tracrRNA/Cas9 Ribonucleoprotein (csRNP...first step of optimization, we started off by using custom synthetic two-part cr/tracrRNAs for CRISPR experiments...tagging, pick a crRNA that cuts 8-15 bp downstream of Stop codon; for N-terminus tagging, a crRNA cutting 8...csRNP experiments, it can be ordered in bulk and stored in small aliquots at -80 oC. Assemble csRNP: Anneal...near the insertion site (within ~8-15 bp from the stop codon for C-terminus tagging) GC content of the ... hands, delivering Cas9 protein in complex with custom synthetic cr:tracrRNAs (csRNP) is the most efficient...

  40. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...not necessarily represent the views of Addgene. Boston, MA, USA 42.3601°N 71.0589°W – Alina Chan The ...dabbles in science policy, public outreach, and restorative justice.  Follow her on twitter @sung_vanessa...to join the public conversation and to tell the stories behind our research.  That's why I marched for ...Michigan, where programs like the Great Lakes Restoration Initiative are at risk of major cuts and where...behind these two fingers the ninety-nine cubits of Aristotle, nor would you mention my small error and at the...second and that it is our duty to tell our science stories in a manner that encourages open discourse with...

  41. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...Emission Depletion (STED), and Stochastic Optical Reconstruction Microscopy (STORM). These dyes have specific...Throughout their history, fluorescent dyes have enabled the visualization of both organic tissues and...than was absorbed. This phenomenon is known as the Stokes Shift, or the difference in wavelength between ...technologies in fluorescent dyes/ligands Throughout the history of cell biology, fluorescent labeling techniques...fluorescent protein Fluorescent Proteins 101: A History of Fluorescent Proteins ...

  42. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...skeptical reader of historical fiction (I can personally attest to this!). Following history from the Ice Age...Wrong - and the New Research That's Rewriting the Story by Angela Saini "This book is a smart and carefully...Strange & Mr. Norrell by Susanna Clarke "A quasi-historical/fantasy fiction. Two dueling magicians in the...of England by Edward Rutherfurd "A 10,000 year history of southern England. While the length may be intimidating...

  43. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ... examples with plasmids deposited at Addgene.  PRESTO-TANGO: The ligands that activate many GPCRs are...Roth’s lab created an open source resource called PRESTO-TANGO (parallel receptorome expression and screening...transcriptional output - TANGO) (Kroeze et al., 2015). PRESTO-TANGO uses a modified Tango beta-arrestin recruitment...ligands for unknown GPCRs. You can find the entire PRESTO-TANGO kit at Addgene! TRUPATH: Bryan Roth’s lab...McCorvy JD, Giguère PM, Sciaky N, Roth BL (2015) PRESTO-Tango as an open-source resource for interrogation...

  44. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...initial reversal drive cannot restore the exact original sequence, it can restore the original protein-coding...are eliminated by natural selection, gene drives distort inheritance in their favor and consequently can...they should not be relied on exclusively because historical studies of pathogen research have conclusively...using a recoding strategy; a subsequent drive can restore the wild-type sequence (save for the residual sgRNAs...

  45. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...Fast-FT, and mK-GO. Large Stokes Shift (LSS): Stokes shift (named after George G. Stokes) is the shift in wavelength... most FPs, Stokes shift is less than 50nm (often much less).  For LSS proteins, the Stokes shift is ≥ ...

  46. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...Ampicillin Ampicillin, often just called amp, is the historical king of the antibiotic resistance realm. Amp ... factor. Zeocin is handy because it can be a one-stop shop for selection across systems and species. The...entirely detoxified, even when Sh ble is expressed (Trastoy et al). Thus, it may not be a good choice for experiments...Hadley, W. K., & Brooks, G. F. (1986). Effect of storage temperature and pH on the stability of antimicrobial...11, 56. https://doi.org/10.1186/1475-2859-11-56 Trastoy, M. O., Defais, M., & Larminat, F. (2005). Resistance...

  47. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ... wrong. It brought the whole field to a grinding stop and only now, 2 decades later, is it finally back...showing that AAV-mediated delivery of the RPE65 gene restores vision in the dog model of LCA (Lancelot, one ... 10 more years to obtain FDA approval and make history in the form of Luxturna™. A better vector for ... again 3. Acland G. et al., 2001. Gene therapy restores vision in a canine model of childhood blindness...PubMed PMID: 11326284. 4. Bennett J, 2017. Taking Stock of Retinal Gene Therapy: Looking Back and Moving...

  48. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ...when balancing a handful of demanding courses, a capstone paper I really wanted to hit out of the park, .... I need it just as much in my current role as Customer Support and Operations Manager at Addgene. In ...about to keep silent and move on to other things…. Stop! Take a step back and think of a time you’ve lead...collaborator or co-worker and showed that you really understood their side of an issue. Maybe you also showed...article from The Muse. Kai Strickland is a former Customer Support and Operations Manager at Addgene and ...

  49. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...celebration, we thought it only fitting to share some stories of ways we’ve turned the available into the accessible...countries, import and export regulations, differing customs processes, and the distances the packages needed...other things, could ensure our packages navigated customs and arrived at their destination in a timely manner... to Design a Primer Creating Bacterial Glycerol Stocks Inoculating a Liquid Bacteria Culture Collections...scientists, from techs to grad students to professors, stopping by to say, “Everything I know about virus, I learned...

  50. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...chose needed to fulfill certain requirements: Customizable to our specific needs Modern and well-supported...the order and usage of the scripts needs to be customized in the pipeline for the specific needs of the...Since each process can use a unique container, we customized each container for its particular purpose and...bbduk process.    At first, to make sure we understood the concept of channels and implemented the input...user interface. In our next post, we’ll share our story about how we used Nextflow Tower to augment our ...
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