We narrowed to 133 results for: plasmid for e coli

... Blog Plasmid Cloning by PCR Perform Site Directed Mutagenesis by PCR Catch up on Your Plasmid Background...transformed directly into chemically competent E. coli and transformants selected with the appropriate... have been isolated from methylation competent E. coli for this procedure to work properly. Directly transform...appropriately sized product, purify and sequence the plasmid to confirm no mutations were introduced during ...Background with our Plasmids 101 Posts Resources on Addgene.org Find Empty Vectors for Your Research Find...Browse Our Special Collections for Plasmids from Your Field ... plasmid cloning...

... resources on the Addgene blog Plasmid 101 Protein Expression Plasmid 101 Biotinylation Other resources...Burke Lab (Roux et al., 2012). BioID utilizes the E. coli biotin ligase, BirA*, with a site mutation (R11G...-binding domain (233 amino acids) found in the E. coli version. It also contains a single mutation (R40G...to noise ratio 30-fold compared to the standard E. coli BirA* mutant. RapID consists of this mutant BirA...vivo studies (Che and Khavari, 2017). Find APEX plasmids at Addgene! Since the development of BioID and... specific locations in the cell.   Find BioID2 plasmids here APEX2: A more sensitive ascorbate peroxidase... They named this mutant APEX2.  Find the APEX2 plasmids! TurboID and minturbo: Rapid and non-toxic proximity...

...blog Plasmids 101: What is a plasmid? Plasmids 101: Blue-white screeningPlasmids 101: Common lab E. Coli... gram negative bacteria include salmonella and E. coli. Because of their thicker peptidoglycan layer, ...useful in a laboratory setting. When designing a plasmid to be inserted into a specific bacterium, it can...it is being used in, and how components of the plasmid should change based on bacteria type. The gram ...

...first deposited set of plasmids containing SARS-CoV-2 protein coding genes in E. coli and S. cerevisiae expression...What SARS-CoV-2 plasmids from Ginkgo Bioworks are available for request? Currently, plasmids containing all...available. Plasmids are continually added as they complete QC and you can find all available plasmids listed...to fresh data! These same plasmids are then added into our normal full plasmid NGS queue in order to bring...pipeline. For the first ~50 plasmids we perform our standard full plasmid sequencing by NGS. Addgene’s...on Addgene.org Find all plasmids from Ginkgo Bioworks  Find COVID-19 plasmids for your research Browse... all SARS-CoV-2 protein coding genes in E. coli and S. cerevisiae expression vectors are available at ...

...recommended for plasmids up to 25 kb, most cloners should be fine, especially using plasmids in the 10-12...Other Plasmid Cloning Techniques like SLIC and Golden Gate Cloning Learn How to Verify Your Plasmid Read...Reference Pages Choose Your Perfect Plasmid Backbone Find Plasmids for Your Research ...nonmethylated PCR products are then transformed into E. coli to undergo nick repair. This method can work well...relying on in vitro assembly, they could make the E. coli do the work by using the principles of recombineering...made genomic SDM relatively straightforward, but plasmid-based SDM has lagged behind. While commercial kits... REPLACR (Recombineering of Ends of Linearized Plasmids After PCR). Current site directed mutagenesis...

... acids E. coli and Mammalian Cells pEVOL-pAzF synthetase for p-azido-l-phenylalanine E. coli pEVOL-pBpF...cell types (E. coli and Xenopus oocytes, mainly). Nevertheless, tRNA suppression plasmids are available...Resources on Addgene.org Find Plasmids for FRET Browse Empty Plasmids to Fluorescent Protein Fusions ...-pBpF synthetase for p-benzoyl-l-phenylalanine E. coli pAcBac1.tR4-MbPyl pyrrolysyl-tRNA synthetase ...the potential rewards could be substantial: Plasmid tRNA Synthetase Expression Host pMAH-POLY Polyspecific...Fig. 1) which can be carried out using Addgene plasmids. His-tag labeling reagents You may already have...

...recorded plasmid multimerization in the naturally occurring E. coli plasmid ColE1 (Goebel & Helinski, 1968...Addgene blog:  Plasmids 101: What is a plasmid? Plasmids 101: Origin of ReplicationPlasmids 101: PCR...process known as plasmid multimerization. Plasmid Multimerization Early studies on plasmid topology recorded...ligate the plasmid, and re-transform the ligated plasmid to recover the monomeric form of the plasmid. Have...multicopy plasmids harboring large inserts are often dimers in Escherichia coli K-12. Plasmid. 1989 Mar... formation of plasmid multimers (Bedbrook & Ausubel, 1976; Fishel et al., 1981). E. coli strains such ... monomer of Plasmid 12259: pMD2.G (depicted on the left) is ~5.8 kb in length. The plasmid can exist on...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...promoter.  They found that the system is active in E. coli and closely related species, is amenable to use...be adaptable to distantly related species from E. coli. This tool should serve as both a powerful tool...need. If you'd ever like to write about a recent plasmid deposit please sign up here.        A retroviral...treatment of cardiovascular disease. Find the LipoGlo plasmids at Addgene! Lawler et al, QJM: An International...promoter systems. Find these CRISPR activation plasmids at Addgene. Liu et al., Nature Communications,...

...this case, plasmid copy number is the input and gene expression is the output. The input, plasmid copy number...to decouple gene expression and plasmid number. In a suite of plasmids modified from pSC101 with copy ...of all types of plasmids, the stabilized promoters were also tested in different plasmid backbones that...stationary phase, the number of plasmids per cell increases 4-5 fold (for pUC plasmids) or ~2 fold (for p15a, ...RepA is a plasmid replication initiator protein, they could alter copy number of the plasmid by altering...genome or a plasmid. Thus, a stabilized promoter can allow a system to be moved from a plasmid to the genome...special collections page Download the Plasmid 101 eBook See all plasmids deposited by the Voigt lab ...

...two nutritional deficient E. coli strains that resulted in a wild type E. coli (Griffiths et al., 2000)...don't exactly "eat" plasmids as depicted in this comic, when a bacterium uptakes a plasmid, the genes on the... the plasmid can give them different phenotypes. For example, this plasmid encodes a green fluorescent... Read all of our Plasmids 101 blog posts Check out all of our blog posts on plasmid cloning Resources...comes from adjacent lysed bacteria and can include plasmid DNA or fragmented DNA released into the environment...membrane so the bacteria can easily uptake your plasmid of interest. Scientists can also use electroporation...exist as transient extrachromosomal DNA, like a plasmid, or it can integrate into the host bacterium’s ...

...specific mutations in your plasmids Use REPLACER Mutagenesis to mutate your plasmid Resources on Addgene....polymerase I (PolI3M) is capable of mutagenesis in E. Coli, but this approach requires special bacterial growth...mutations per nucleotide per generation of wild-type E. coli, while only increasing the standard mutation rate... targeted mutagenesis experiments? Find EvolvR plasmids at Addgene! References Gaj, Thomas, and Pablo...Browse our CRISPR guide pages Find bacterial CRISPR plasmids ...

... usage bias in Plasmids 101: Codon usage bias Read more about plasmids in our Plasmids 101 blog series...with codon bias across all endogenous genes in E. coli and S. cerevisiae (Tuller et. al., 2010). ...codon optimizing a human gene for expression in E. coli. Genes are also optimized for expression in mammalian...Reference Read about genetic code expansion Browse our plasmid collection page  ...Optimization References Athey J, Alexaki A, Osipova E, Rostovtsev A, Santana-Quintero LV, Katneni U, Simonyan...rna.02890211 Tuller T, Waldman YY, Kupiec M, Ruppin E (2010) Translation efficiency is determined by both...

...opposite, a uracil DNA N-glycosylase (UNG) from E. coli. The resulting construct, eUNG-BE4max(R33A)ΔUGI...out of 30 target sites. But the same strategy in E. coli produced different results. GBEs seemed to follow...— by excising the freshly edited U bases — but E. coli instead filled those abasic sites with A's, producing...). In addition, they showed that UNG from both E. coli and C. elegans could be effective components of...Additional resources on Addgene.org Browse CRISPR plasmids for base editing Get a broad overview with our...uracil bases in DNA. References Chen, L., Park, J. E., Paa, P., Rajakumar, P. D., Prekop, H.-T., Chew, ...https://doi.org/10.1038/s41467-021-21559-9 Huang, M. E., Qin, Y., Shang, Y., Hao, Q., Zhan, C., Lian, C.,...

...from the bacterial genome, and inserted it into E. coli to express and then test for any proteins that ... addition, when they looked at the transformed E. coli expressing Cas7-11, they found no evidence of growth...Use PASTE can be delivered via a single dose of plasmids, works in non-dividing and primary cells, and .... They classified the system as Class I, Type III-E, and then used this information to identify a large...Resources References  Özcan A, Krajeski R, Ioannidi E, Lee B, Gardner A, Makarova KS, Koonin EV, Abudayyeh... John A. Walker II, Anastasia P. Kadina, Adrianna E. Zepeda, Kevin Holden, Jonathan S. Gootenberg, Omar...

[email protected]. If you are looking for plasmids used in E. coli and yeast, you’re in luck! Addgene has...Collection page, you can browse for plasmids by species and find our guides, plasmid kits and other resources for...microbial research. Kits, guides, plasmids, and more We have Plasmid Kits for microbial work with fluorescent... To make it easier for you to find plasmids for your work, plasmids in the Microbiology Collection are... a useful plasmid that’s not in the collection? Tell us about it with our Suggest a Plasmid Form! Finally...develop resources for your community! To contribute plasmids made in your lab, see www.addgene.org/deposit ...

...supplemental add-on kit and is compatible with E. coli and/or cell-free systems. Protein expression in... al. “EcoFlex: A Multifunctional MoClo Kit for E. Coli Synthetic Biology.” ACS Synthetic Biology, vol....MoClo: Improved MoClo Assembly Standard and New E. Coli Part Library Enable Rapid Combinatorial Design ...between CRISPR components expressed from different plasmid backbones. They noted that backbone differences... as the order, or distance in the placement of plasmid elements can impact expression levels and consequently...system was used to assess how different lentiviral plasmid backbone elements impact viral titer and to compare...plants, and animals (Chiasson et al., 2019). Many plasmid elements such as promoters or fluorophores are ...

...Deletions in a Plasmids Learn How to Verify Your Plasmid Resources on Addgene.org Find More Plasmid Cloning... reaction into chemically or electro-competent E. coli - excess salt causes arcing during electroporation...vaccinia virus DNA topoisomerase I in Escherichia coli is sequence specific."  Proc Natl Acad Sci U S A...

... involving two-plasmid or single-plasmid systems (Vo et al., 2020). In the two-plasmid system, an effector...separate plasmids and multiple promoters were used to express the necessary components in E. coli (Klompe...temperatures. Data adapted from Vo et al., 2020. In E. coli, the new streamlined VchINT system was shown to...effector plasmid (pEffector) expresses all seven required genes and the CRISPR RNA under the same promoter...promoter, and a donor plasmid (pDonor) encodes the mini-transposon. pEffector and pDonor can also be combined...combined in single-plasmid INTEGRATE (pSPIN), which offer simple transformations for smaller cargos, and ...and the ability to easily cure cells of the plasmid after integration. Figure 2: (A) Streamlined ...

...), 339-350 (2015). PubMed. Find the Plasmids @Addgene: Plasmids for CRISPRi are described here: http...metabolic engineering realm of S. cerevisiae and E. coli. The utility of simultaneous and multidirectional...overexpression of various metabolites in Escherichia coli or Saccharomyces cerevisiae with few techniques ...constructs to be introduced simultaneously in a single plasmid. The functionality of the RNA scaffolds follows...http://www.addgene.org/crispr/qi/ Plasmids described in  Zalatan et al. Cell  (2015) can be found here...https://www.addgene.org/browse/article/9593/ Plasmids described in Qi et al. Cell  (2013) can be found...

...construction of new plasmids. Dr. Duedu will also use polymerases produced using plasmids from Addgene to ...tools to construct chimeric cellulases that enable E. coli and Citrobacter freundii to generate sugars from... has been working with Seeding Labs to provide plasmid grants to researchers in developing countries. ... to announce that we’ve selected our first two plasmid grant awardees: Drs Louis Bengyella and Kwabena...doctors Bengyella and Duedu and how they plan to use plasmids from Addgene to advance their research. Louis ...cellulosic biomass. Dr. Duedu would like to use the plasmids he acquires through Addgene to produce molecular...fluorescent proteins. Dr. Duedu’s plans for these plasmids have a heavy focus on teaching. He hopes to teach...

...format that’s easier to express in the cytoplasm of E. coli, although this is not possible for all scFvs. ...insect cells, they are most often expressed from a plasmid in bacteria (Ahmed et al., 2012). One challenge...single-chain Fv analogue produced in Escherichia coli. Proceedings of the National Academy of Sciences...Sivaccumar JP, Ruvo M (2020) Evolution of Escherichia coli Expression System in Producing Antibody Recombinant...Resources on Addgene.org: Browse Addgene’s Antibody Plasmid Collection Find antibodies for neuroscience research...Novotny J, Margolies MN, Ridge RJ, Bruccoleri RE, Haber E, Crea R (1988) Protein engineering of antibody binding...

.... pBLADEONLY: This plasmid contains only BLADE and is based on the pTRC99a plasmid. It does not include...called BLADE (blue light-inducible AraC dimers in E. coli) that turns expression on and off with blue light...AraC and other promoter systems, head over to our Plasmids 101: Inducible Promoters article.) Modifying AraC...reporter downstream of the PBAD promoter. To use this plasmid with your gene of interest, you would replace mCherry...PBAD promoter. If you already have a pBAD33-based plasmid expressing your gene of interest, all you have ... and shared with Addgene. You can find all the plasmids deposited from Di Ventura's lab, including the...nuclear export system (LEXY) here. Optogenetic plasmids from the Khammash lab can also be found here. ...

...conjugative mobilization of broad host-range IncQ plasmids." Plasmid 62.2 (2009): 57-70. PubMed PMID: 19465049...page Deposit microbiology plasmids with Addgene Find synthetic biology plasmids ...determined. The lab used DCDC to measure growth rate of E. coli for over 10 generations and showed that this method...such as Snodgrassella alvi and Gilliamella apicoli. The plasmids could also be transformed into Alphaproteobacteira... With MAGIC, scientists introduce an Escherichia coli donor strain into the microbiome to deliver a genetic...family-RP4 conjugation system. This system can deliver plasmids to both Gram-positive and Gram-negative cells.... Wang lab further developed a modular suite of plasmids (pGT) with various replicative origins that can...

...Not all plasmid preps are the same. Before purifying a plasmid from a bacterial culture, it is important... into a plasmid backbone. Transfection grade DNA Figure 3: Need to introduce plasmids into mammalian... an external plasmid in mammalian cells and it will likely require micrograms of plasmid DNA for each ...utilize your plasmid DNA, so that you can decide what will be the optimal type of plasmid preparation ...Addgene blog Browse blog posts on plasmid cloning Read our Plasmids 101 blog posts Learn about the differences...of the cell wall of Gram negative bacteria like E. coli that affects tissue culture cell line viability...purity. Based on these premises we can classify a plasmid preparation in 3 different ways: Transformation...

... underway in the Ellis lab including yeast and E. coli engineering, microbial cooperation, and antibiotic...Synthetic Biology Education Nonprofit BioBuilder Plasmids 101: Golden Gate Cloning Learn About Working at...Michael Koeris Resources at Addgene.org Find Plasmids from the Ellis Lab Find Synthetic Biology Kits...

...containing the putative tracrRNA into a plasmid and expressed it in an E. coli bacteria strain. They found that...sheet Resources on Addgene.org Find the Cas14 plasmids here Checkout our CRISPR guide page Browse the...

...Neurosci 2016. PubMed PMID: 26950006 Plasmid ID Plasmid Name Plasmid Type 74309 pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry...the process of generating multiple E. coli gene expression plasmids with the Cross-disciplinary Integration...creation of many E. coli constructs with greatly varied levels of expression. The 93 plasmids in the CIDAR...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These brief articles...features and applications of a partiular plasmid or set of plasmids. We hope that these articles make it ... you to find and use the plasmids you need. You can find all the hot plasmids from 2016 below. With over...over 45,000 plasmids, we can't write posts for every great plasmid that comes into the repository, but...

... successfully takes up plasmid DNA. Among those that do, some now have plasmids that contain your gene...including ccdB on its destination plasmids. In the destination plasmid, the ccdB gene is flanked by recombination...selections in plasmid cloning. Additional resources on the Addgene blog Read our Plasmids 101 blog posts...synthesizes the critical amino acid). Many common E. coli strains used for cloning are auxotrophic for specific...purify your gene of interest, get it into your plasmid backbone, and zap the mixture of DNA into cells...gene of interest, but others will uptake plasmid backbones that re-ligated back on themselves. Therefore...strategy needs to identify cells containing the plasmid construct you’re seeking. Fortunately, there are...

...purification process simpler. Due to its high yields, E. coli is often used for the production of Fab fragments...Randolph TW, Schiltz E, Sederstrom D, Steinmann D, Mozziconacci O, Schöneich C, Freund E, Ricci MS, Carpenter... great collection of ready-to-use rAbs and rAb plasmids!). Since recombinant antibodies conveniently allow...antibodies can be produced through a single or dual-plasmid transfection, depending on whether the heavy and...and light chain genes are contained to one plasmid or if they’ve been separated onto two. You’ll first...first transfect your cells with your plasmid(s), and then wait 7-14 days (in mammalian cells) to collect ...on the Addgene blog Introduction to Antibodies Plasmid-based Recombinant Antibodies Polyclonal Antibodies...

...Blog Learn New Plasmid Cloning Techniques Pushing the Limits of DNA Assembly Plasmid Cloning by PCR ..."Last stop on the road to repair: structure of E. coli DNA ligase bound to nicked DNA-adenylate." Molecular...including vector-insert joining for recombinant plasmid construction (restriction cloning), adaptor ligation...thermophilus DNA ligase, a homolog of Escherichia coli DNA ligase." Journal of bacteriology 173.16 (1991...757-764. PubMed PMID: 15582400. 2. Tomkinson, Alan E., et al. "DNA ligases: structure, reaction mechanism...PubMed PMID: 16464020.  3. Ellenberger, Tom, and Alan E. Tomkinson. "Eukaryotic DNA ligases: structural and... PubMed PMID: 17466627. 25. Luo, Jianying, Donald E. Bergstrom, and Francis Barany. "Improving the fidelity...

... CRISPR sequences were initially discovered in E. coli in 1987 (1), the concept that these clustered repeat... in the field, and enabling access to multiple plasmids used for various platform applications. For more... out Addgene’s CRISPR Guide or find CRISPR/Cas plasmids for your research. Do you have a cool idea or ... K, Haft DH, Barrangou R, Brouns SJJ, Charpentier E, Horvath P, Moineu S, Mojica FJM, Wolf YI, Yakunin...

...Unpublished or pre-publication plasmids": Unpublished plasmids or plasmids that will not be published are...and entering a plasmid name,plasmid type, and plasmid description/experimental purpose (right). On the...with your plasmids, and adding all the plasmids at once increases the likelihood that the plasmids are given...multiple plasmids and would prefer to send us your plasmid information in spreadsheet format, e-mail us... if your plasmid is designed to allow you to produce large amounts of your insert in E. coli, then this...easy for you to share plasmids. To achieve this goal, we will archive any plasmids you've deposited with...Spreadsheet". Start your plasmid deposit There are two options to begin your plasmid deposit. Figure 2: Choose...

... Hot Plasmid list. Other popular plasmids & blog posts @Addgene: The 10 Most Distributed Plasmid Technologies...protein in Rosetta E. coli. New Lentiviral CRISPR activator and repressor plasmids from Scot Wolfe's lab...constrained only by the ability of E. coli to maintain the final plasmid after transformation. Addgene depositors...inducible inactivation. Find the Robinson plasmids or the Royle plasmids and get to work! Robinson et al...designed the pOSIP (one-step integration plasmid) series of plasmids, vectors that convey both the sequence...mGRASP plasmids. Additionally, the lab recently described the use of another set of mGRASP plasmids in their... favorite plasmid technologies? Email us at [email protected]  and let us know what plasmids you would ...

...the newspaper, Addgene uses E. coli bacteria to generate copies of plasmids. The community of scientists... into the successful, internationally renowned plasmid repository that it is today. Although I wish I ...music playing all night was hand-picked (like our plasmid samples!) by Addgenies. If you were there, you ... complicated process of requesting and sharing plasmids has become simplified through Addgene. Looking...Check out our impressive stats from 10 Years of Plasmid Sharing Or read more about what happens Inside ...

...cells to retain plasmids - if a cell does not inherit plasmid due to improper plasmid partitioning, it...unstabilized plasmids were retained in less than 10% of cells while plasmids containing the plasmid addiction...ensure that plasmids are passed down through generations (Figure 1). These so called “plasmid addiction ...only survive if they retain the plasmid. Since their discovery, plasmid addiction systems have been used...found that if a mini-F plasmid carried a specific 700 bp segment of DNA, the plasmid was propagated better...selection in one plasmid, they were able to create an efficient cloning tool. The plasmid pKSCTMF contains...endonucleases in plasmid addition was first described by studies of EcoRI and Bsp6I in Escherichia coli in 1995...

...Browse our CRISPR interference plasmids Find bacterial CRISPR plasmids Read our CRISPR guide ...thetaiotaomicron, Mycobacterium, Pseudomonas, and E. coli. However, many non-model bacterial species lack...transposon system. This system has two parts: a plasmid expressing the Tn7 transposition genes (tnsA, tnsB... tnsB, tnsC, tnsD, and tnsE), and a plasmid that contains the Mobile CRISPRi cassette flanked by left ...copy of the RP4 transfer machinery mobilize these plasmids for transfer to recipient cells during a tri-parental...Mobile-CRISPRi ICE element forms a conjugative plasmid-like DNA circle and is transferred to recipient...transposon containing the CRISPRi components and a plasmid with the Tn7 transposition genes are transferred...

... Can I use my favorite plasmid with this protocol? Maybe. Not all plasmids will work for the one-pot...strands of the plasmid will be nicked during the first BbvCI digest and the whole plasmid will be degraded...those in the plasmid. Again, if they are in the opposite direction, both strands of the plasmid will be nicked...map of your plasmid. If the sites are in opposite directions, you just need to use a plasmid with BbvCI...Mutagenesis to Mutate Your Plasmids Resources on Addgene.org Find Plasmids from the Whitehead Lab Use...the plasmid. The ssDNA template is generated by transforming a backbone phagemid vector into an E. coli...Wrenbeck, Emily E., Justin R. Klesmith, James A. Stapleton, Adebola Adeniran, Keith E J Tyo, and Timothy...

...library of E. coli tRNA adenosine deaminases (ecTadA), fused it with dCas9, and transformed it into E. coli...all CRISPR plasmids and resources at Addgene Find CRISPR base editors Find CRISPR plasmids for plants ...

...University of Oregon created mTFP1 by transforming E. coli with a gene library of approximately 484 variants...Biotechnology which allows researchers to deposit plasmids containing the fluorescent protein mNeonGreen....with other fluorescent proteins. Find mNeonGreen plasmids here! mTFP1: a monomeric teal fluorescent protein...physiologically relevant changes in pH. Find mTFP1 plasmids here! mWasabi: a bright green fluorescent protein...excite at 400 nm while EGFP does. Find mWasabi plasmids here! One of the most notable things about these...are best suited for certain studies. If you have plasmids containing mNeonGreen, mTFP1, or mWasabi that ...with the scientific community, please deposit the plasmids at Addgene! References Ai, Hui-wang, et al. "...

...in a 1-plasmid system, in which Cas9 is included on the gRNA-containing plasmid, or a 2-plasmid system...contain thousands of gRNA plasmids, discerned only by a unique barcode on each plasmid. As such, sequencing...CRISPR libraries that target mouse, human, fly, E. coli, and T. gondii genes.  2) What genetic modification...are composed of many different gRNA-containing plasmids, each with a different genomic target. The goal...sequences and ease of generating gRNA-containing plasmids. CRISPR genome wide-screening experiments commonly...other types of experiments, check out our CRISPR Plasmids and Resources Page. References McDade JR, Waxmonsky...Lentivirus Get Tips on Titering Your Lentivirus Plasmids 101: NGS Quality Control for Pooled Libraries ...

...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  ...construct can be easily expressed and purified from E. coli, retains important functional properties, and is... or want to swap out the constant domains, the plasmids for the heavy chain and light chain are also available...ubiquitination and degradation.   Find the CRBNmidi plasmid here!   Kroupova, A. et al. (2024). Design of ...minimal background.   Find these C. elegans mScarlet plasmids here!   Cao W.X., Merritt D.M., Pe K., Cesar ...disruption in Mycobacterium abscessus using a dual-plasmid CRISPR/Cas9 system By Aryana Khosravani Deborah...Deborah Hung’s lab has developed a novel dual-plasmid CRISPR/Cas9 system for gene disruption in Mycobacterium...

...nights :D) engineering E. coli to produce biofuels in Pamela Silver’s Lab (plasmid page here). Now I spend...helping scientists share information and, of course, plasmids. My primary duties are to manage this very blog...interesting than that will simply get people ordering plasmids. The need to keep content coming also keeps me...talking to researchers about their work, picking up plasmid deposits, and trying to learn ways Addgene can ...

...underrepresented plasmids (plasmids that were lost or “dropped out”) and overrepresented plasmids (plasmids that ...In addition to single plasmids, Addgene also distributes pooled plasmid libraries containing hundreds...library contains many plasmids, care must be taken to ensure that all of these plasmids are amplified and ...diversity. If the various plasmids in the library are differently sized, smaller plasmids may be amplified preferentially...101 Library amplification seems straightforward: E. coli bacteria are transformed with the library DNA, ...hundreds, thousands, or even a million plasmids. These libraries are some of Addgene’s most exciting and versatile...harvested, much like one would prep an individual plasmid. However, lots of library DNA is needed for screening...

...engineer 14 chromoprotein sequences into E. coli BioBrick plasmids (meffRed, eforRed, asPink, spisPink, scOrange...at the cells on the plate. For example, if your plasmid contains a gene encoding a blue chromoprotein, ...colonies to determine which cells have taken up the plasmid. Of course, other follow up methods like sequencing...chromoproteins. In some cases, cells containing high-copy plasmid-borne chromoproteins lost color overnight in liquid...problem. There is no single “ideal” chromoprotein plasmid that combines the most desirable features: intense...Chromoproteins for allelic exchange Figure 3: pTOX plasmids expressing amilCP and tsPurple genes. Image: Lazarus...sequential homologous recombination events: The plasmid carrying the desired genetic modification integrates...

...proteins. She also showed that R-bodies can rupture E. coli membranes. Read the full post for potential applications...Nathaniel Roquet from the Lu Lab at MIT developed plasmid based systems that report the order in which specific... plasmid...

...Two-Component Systems for E. coli Browse other blog posts from our Plasmids 101 series Resources on Addgene.org...Resources on the Addgene Blog: Plasmids 101: The Promoter Region Plasmids 101: Cre-lox Light-Switchable...Promoter Subtype Promoter Example Activator Addgene Plasmid/Kit Alcohol-regulated AlcA promoter AlcR pGGA008...use light to regulate transcription. Red flame plasmid pDawn contains the blue-light sensing protein YFI...Inducible Systems at Addgene Cre-lox Guide and Plasmids     ...

... If there’s a plasmid or AAV tool that you can’t find, you can always suggest a plasmid to us, request...you're currently a synthetic biologist engineering E. coli metabolism, maybe you’ll be able to apply your ...want to get into the field. Find Optogenetics Plasmids for Use in Neuroscience Research Neuroscience ...400 who signed up for our newsletter and a free plasmid) were very excited about our new viral services...Service Find Retrograde AAV Browse Optogenetics Plasmids ...

...Browse our Plasmids 101 blog posts Read blog posts on plasmid cloning Read about different plasmid elements... not work. Check the plates! Streak on them an E. coli  strain known to be sensitive to that antibiotic...to the backbone plasmid; a starting point could be a molar ratio of 1:2 (plasmid:insert), but this parameter...colonies screened might not contain the correct plasmid. There’s a lot that can go wrong! With all the ...cloning project, and hopefully obtain your coveted plasmid with no substantial delays. Design, design, design...spend some time outlining the construction of the plasmid and all the steps you will have to take. Decide...you chose, and verify that the sequence of the plasmid you are working with is correct. It's also best...

...2021 Plasmids are used to express 10, 15, 20, 30, 40, 50, 60, 80 and 100 kD proteins in E. coli, either...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Here's what you'...Ladder  by: Rachel Leeson This simple, inexpensive plasmid-based ladder system is a cost-effective alternative...kD proteins or 20, 40, 60, and 80 kD proteins. Plasmids containing 150 or 250 kD proteins are also available...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...write about a recent plasmid deposit please sign up here.  Listen to the hot plasmids episode! New, highly...hundred SH3 domain containing plasmids available through Addgene. These plasmids can be used to carry out ...function. RNAi is commonly achieved by feeding E. coli expressing dsRNA to C. elegans. These dsRNAs target...dsRNA necessary for RNAi. The Vellai lab’s new plasmid, T444T, contains T7 terminators adjacent to the...to increased RNAi efficiency. Find the T444T plasmid here! Strum A, et al. Nucleic Acids Research. 2018...