...neurodegeneration research: Anti-MAPT [AT8] and Anti-APP [6E10-A5]. Both are recombinant mouse monoclonal ...Figure 5: Immunohistochemistry with Addgene's Anti-APP [6E10-A5] recombinant antibody in human postmortem... antibodies here! Wu, Z., Wang, W. (2024). Anti-APP [6E10-A5] in Immunohistochemistry (Human). Addgene...in human cells, has been uniquely challenging to apply in dicotyledonous plants, or dicots. But Jae-Yean...efficiency barrier in tomatoes" by combining several approaches (Vu et al., 2024). Their optimized strategy uses... wild type (WT), two weeks after herbicide was applied to both plants. Adapted from Vu et al., 2024 bioRxiv...CC-BY-NC-ND 4.0 International license. With this approach, the team was able to drive prime editing in both...

...Antibody, Addgene gave to me…  A recombinant Anti-APP!   🎶On the eleventh day of Antibody, Addgene gave...

...plan out your agenda. Some conferences even have apps you can use to build a schedule. If there is a list...meet. Some conferences may also have a dedicated app with a social media component, but if it doesn’t ...up, create one yourself. Pick a time (ex: lunch, happy hour) and use the conference hashtag to set one ... this, you probably notice a trend: networking happens everywhere! Even when you aren’t “trying to network...

...Save Money with the Baby Gel Box Find 5 Smart Phone Apps for Lab Life Learn About the Evolution of Lab Techniques...

...other tools they use in research. This includes chat apps like Slack, Microsoft Teams, and Google Hangouts...and science-specific tools, e.g. sample management apps, colony management software, and method repositories... take a look at the RSpace ELN.  The connected approach in action: how the RSpace ELN supports reproducibility...

...Finally, my main content organizer is the amazing app Evernote. I have a paid account which gives me all...for Scientists Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments Additional...to find a way to keep doing science to be happy. The happy path is not the academic one for the majority...say that doing a Reddit “Ask Me Anything” was appealing, but also scary. I do a lot of public speaking...leaving out the term “implicit bias” which is apparently a troll trigger phrase these days). Redditors...database. Still have questions? I am easy to reach and happy to help. Additional Resources on the Addgene Blog...

....  Additional Resources on the Addgene Blog Use Apps to Save Time and Track Your Experiments Use video...glean from a reading session. To remember what happened when drug X was added to cell line Y, or the phenotype...

... carry a notebook at all times (or use the notes app on your phone) to capture fleeting thoughts and connections... your intellectual journey. Practical approaches One approach to achieve this goal could involve participating...often happen when we allow ourselves moments of release and mental freedom. Practical approaches Engaging...predefined path, opening doors to novel ideas and approaches. Moreover, the ability to embrace serendipity....” This quote encourages us to contemplate our approach to life and how our viewpoints influence our encounters...

...something else to focus on. Use collaborative cloud apps It is a good idea to use collaborative cloud tools...miscommunication, a lack of leadership, and inappropriate tools or writing approaches can lead to frustration, delay...hands or signal the approach of a brain break, where everyone stands up, disappears from the video, and...members talk no science. No data presentation will appear here, and only general chatter will be allowed ...social distancing. The take home message is the approaches/tools we have to foster creative collaboration...

... you are interested in.     STEAMid is an app that helps you find internships and scholarships ...student from applying to the opportunities of their dream.  To help students focus on the application process...internships you are applying to, keep your resume under two pages unless you are applying for a Federal Government...your application a whole week before the deadline. The day of submission, review your application with...the resource, and apply as a cohort. This may sound counterintuitive since that approach seems to create...Scrutinize every opportunity prior to starting an application to assess your eligibility, interest, and career...February represent peak season for internship applications. Thus these noble individuals are inundated ...

...invitation. Don’t try to connect from the LinkedIn phone app either as this also results in a generic invitation...out right. Also, many people on LinkedIn don’t appreciate random requests without a personal message and...as spam or “I Don’t Know This Person.” If that happens a few times, LinkedIn may revoke your ability to...post and occasionally comment on their posts as appropriate, possibly giving you a way to connect with them...updates, or click bait of any kind. For more on inappropriate content see this article. However, do comment...

...Notebook for Bioinformatic Analyses Use Software Apps to Save Time and Track Your Experiments Additional...Find the appropriate glycerol stock within our freezers Pick the sample into the appropriate stab culture...corresponding entry in your database. Enjoy your happy lab staff. Barcoded materials make everyone's life... is almost always worth it. Because labels are applied to containers and not the mushy, gooey contents...be!) bear in mind that your barcodes are being applied to the containers, not to the contents. Tightly...contents of a tube with the tube itself. If something happens to the worm that requires a change of tube, there...

...section.   Additional Resources on the Addgene Blog Apps for Lab Life Developing Lab Management Software ...

...titles and abstracts are available online or in an app prior to the session, so bookmark which posters are...background without an introduction. If someone approaches your poster, introduce yourself and ask about... a related topic, and let them know while you appreciate the thought-provoking question, you are unable... enjoy hearing the highlights so that you can appreciate the impact of the project as a whole. In addition...

... scientists––CrispyCrunch! CrispyCrunch is a web app that helps scientists design and analyze batches ...score is less than 0.03, it stops mutating. (This happens often because the HDR inserted sequence splits ...Psychology at McMaster University in Canada. He's happy to be contributing again to science.   Acknowledgements...

...letters to be very important. On the other hand, our app/dev team rarely, if ever, sees cover letters submitted...usually yes! A good cover letter won’t hurt your application, but it can certainly help you stand out.  In...consider cover letters an important part of the application. Even within an organization, there can be variance...good cover letter!) As with any part of the job application process, it's a good idea to talk to people in...do the job as described. Let’s imagine you’re applying to a job that requires ddPCR skills. Your resume... to proactively address questions about your application. These could be logistical, like briefly explaining...  Finally, it’s a good idea to state why you’re applying to the specific company or lab. Take some time...

...Learn about more tools for reproducibility 5 great apps for lab life Developing Lab Management Software ...sciNote. Today, every lab has its own habits and approaches to record keeping. Top priorities in most cases...Project: Cancer Biology have shown similarly disappointing rates of reproducibility. These alarming results...familiar paper-based lab notebook is still a standard approach to record-keeping in many labs. In a way, it will...digital data generated in labs. While many labs approach the situation differently, some adopting simple...might be available as well. In most cases this applies to universities. How are you currently exchanging... Besides this being of crucial importance when applying for a patent for example, it also comes in handy...

...mutations in genes associated with dementia, such as APP and PSEN1, in most cases the intended HDR events ...with blocking mutations, we still weren’t entirely happy with our editing outcomes and in many cases still...

...tools as some kind of a meta/Frankenstein app? Or perhaps there is a clear winner, is there something that...the one hand we have software tools that apply some approximate or sensible rules to determine the value... more efficient.  These insights are hard to appreciate without computational support. Indeed, rational... solutions that facilitate most experimental applications in CRISPR engineering. When compared to that...easy to fault the lack of features in a single application. The question is then, should we use all tools...2013) used a combination of both empirical and approximate methods. The details of their scoring function...and it means that there is no one-size-fits-all approach. You will have to select a tool based on your ...

...knockout approaches. In these cases, we would recommend a knockdown approach instead. In this approach, you...using one or more of the approaches outlined below. Knockout cell line approach Genetic knockout-based...Knockdown approach For cell types where knockout studies are not feasible, an alternative approach is RNA... the target of interest. This approach maps onto the orthogonal approach within the five pillars and is...knockout/ or knockdown approach should be preferred. The overexpression approach is therefore best suited... use of orthogonal approaches, cell treatments, and independent antibody approaches in different assay...target-of-interest in your assay and sample-of-interest. The approaches available broadly map onto the five pillars of...