...Less than 9% of plastics are ever recycled, so adopting a few meaningful changes in the lab could make...to make research more environmentally friendly. Adopt one or all of the recycling practices below. Reuse...

...express a luciferase reporter which is used as a readout for infection of ACE2-expressing target cells. ... MG, Yang D, Miller TE, Feldman J, Hauser BM, Caradonna TM, Clayton KL, Nitido AD, Murali MR, Alter G,...

...sequential excitations wavelengths as determined by Rosado et al: see Table 2 for more details on the effect...variants are available from Addgene. References 1. Rosado, C., Mijaljica, D., Hatzinisiriou, I., Prescott...

...Activate the signaling molecule and perform the assay readout.  Of course, this is much easier said than done...the samples, antibodies, activation, and assay readout used. You’ll note that the secondary antibodies...

...to take. Decide which technique you are going to adopt (i.e. restriction cloning, Gibson Assembly, Golden... has to be optimized according to the strategy adopted, and to the specific reaction you are running. ...

..., calmodulin or troponin C). Their fluorescent readouts are used to monitor calcium concentrations and...sensor option if you have the setup necessary for readout.   We’ve got a live one (calcium sensors...

...happens (this time, Poisson statistics), and the readout gives an absolute value of DNA concentration. By.... PMID: 21554069. Haridan, U. S., Mokhtar, U., Machado, L. R., Abdul Aziz, A. T., Shueb, R. H., Zaid, ...

...concentration of fluorophores) cast shadows along the illumination path. The shadows appear as stripes in the acquired...

....1038/s41597-021-00981-0 Wright, J. F., Le, T., Prado, J., Bahr-Davidson, J., Smith, P. H., Zhen, Z., ...L., Muñoz, K. A., Hsu, R., Kalwani, L., Sierra-Mercado, D., Robinson, J. O., Outram, S., Koenig, B. A....

...from the yeast S. cerevisiae (Gronostajski and Sadowski 1985). The FLP system works in a similar fashion...PMCID: PMC3655711. 4. Gronostajski, R M, and P D Sadowski. 1985. “The FLP Recombinase of the Saccharomyces...

...number of bands present on the strip. This type of readout allows for nucleic acid detection almost anywhere...Servellita V, Singh J, Miao X, Streithorst JA, Granados A, Sotomayor-Gonzalez A, Zorn K, Gopez A, Hsu ...

...escape a threat. Locomotion can thus be used as a readout for associated emotional phenomena like stress ...2018). Locomotion can also be used as behavioral readout to study certain forms of drug addiction. When ...

...approach can be incorporated into a lateral-flow readout slip that allows rapid detection.  Highlights: ...Servellita V, Singh J, Miao X, Streithorst JA, Granados A, Sotomayor-Gonzalez A, Zorn K, Gopez A, Hsu ...

...recommendations for improving antibody use and reliability (Roncador, et al., 2015; Taussig, et al., 2018; Uhlen, et...Nature 518:27–9. https://doi.org/10.1038/518027a  Roncador G, Engel P, Maestre L, Anderson AP, Cordell JL...

...targeted cells. Depending on the cells used and the readout, this could be a major limitation. An alternative...custom CRISPR screen and the appropriate phenotypic readout, similar mapping could be rapidly performed by ...

... the intended target site. With the widespread adoption of CRISPR/Cas9 technology and rapid improvements... field (DAPI). Using the ICC assay as a direct readout of tag insertion efficiency, we have recently demonstrated...

...have the proper controls or a proper behavioral readout that allows you to interpret the results of your... high quality stereotaxic frame with a digital readout. The advantage is you can “zero” your coordinates...

...inducible gene expression Brilliant technologies adopt basic biological concepts and employ them in creative...copies of targeting sequences. Scientists have adopted and employed this concept in both imaging and inducible...observed at a later time via DNA sequencing or other readout. Cellular memory is accomplished by the use of ...

...easy to source, making it easy to switch from one readout method to another or change primary antibodies ...this truly does provide a relative, not absolute, readout. Normalization Overall protein expression varies...

...., Greenbaum, A., Ravi, N., Wu, W.-L., Sánchez-Guardado, L., Lois, C., Mazmanian, S.K., Deverman, B.E....PubMed Central PMCID: PMC2895694. Hollis, E. R. 2nd, Kadoya, K., Hirsch, M., Samulski, R. J., and Tuszynski...