We narrowed to 463 results for: BLI;

...Organizing a science conference for the public How does one organize a public scientific conference? As a member...DayCon is a one-day conference aimed at the general public that provides an accessible introduction to various...the communication gap between scientists and the public and help make science more transparent. Second,... their communication skills and engage with the public. The organization runs a wide variety of programs...conference-style experience that was accessible to the general public both in terms of cost and content level. Sponsorship...science outreach can be, both for scientists and the public. For students, these opportunities to improve our... alternative careers in science. In return, the public receives trustworthy, intelligible scientific resources...

...you are trying to clone. Figure 2: MoClo assemblies depend on compatibility of four-base overhangs...tools When available, always perform your MoClo assemblies in silico before you start doing your cloning...sequentially for your Level 0, Level 1, and Level 2 assemblies using the plasmid sequence files provided with...synthesized DNA fragment can be used directly in your assemblies. Preparing reagents Keep it cool You’ve done ...kit plate for fresh bacteria. Performing your Assemblies Calculate your volumes As you progress through...high throughput You are ready to perform your assemblies, but you have a high number to get through. It... It’s time to think big and perform your assemblies in a high-throughput format. If your lab owns a liquid...

...to be doing in humans; We have not had a broad, public conversation or consensus around the ethics of ...being experimented on, too.) However, as research published on December 13 shows us, CRISPR/Cas9 techniques... us with the second critique. Absent of a broad public consensus on genome editing, there was a generally...revisited at meetings when sufficient time, additional public fora for data review, and more lab research could...could be used to bring scientists and the public together to build consensus guidelines, but there was ...t have to look far for proof that this model of public involvement, research, and consensus can happen...Council. While the Nuffield Council has recently published a report on the social and ethical issues of genome...

...number of organizations in public engagement efforts, including giving public lectures, writing science...disparities. But as the science moves forward at a blistering pace, it is becoming ever more urgent for scientists...between these communities and the biomedical establishment. This, along with concerns about the use of ...coursework and attending seminars in bioethics, public health, science policy, and science and technology...obtaining my PhD and working in digital academic publishing for a year, I’ve since returned to pgEd as a ...organizations (often volunteer-based) that put on public lectures, science cafés or science festivals, and...for science-outreach organizations or student publications, submitting op-eds to your local newspapers,...

... already answered this question and used it to publish!  Working with AAV vectors in neuroscience Let...performance in our system and yet none of that data was published.    Fig. 1: Testing of three different ... for your system or to successfully replicate published works. Of particular importance to these efforts... focus on getting this information out into the public where it can be utilized by others. Addgene’s AAV...citable DOIs through DataCite before being made publicly available through the Data Hub. These minimum ...build on others’ results is available not through published manuscripts, but through word of mouth or old ...asked your team members or colleagues: How much unpublished AAV data do you have sitting in computers and...

...science to the public, talking to policy-makers, and broadly defending science in the public sphere as often...Outside the lab, she dabbles in science policy, public outreach, and restorative justice.  Follow her ...devaluing science has alarming implications for our public policies—many of which impact reproductive rights...forgetting how to engage with friends, family, and the public about its importance.  I think many of us grew ... proposed.  It's our responsibility to join the public conversation and to tell the stories behind our...legislators and because the march had been so well-publicized, we might encounter hostile anti-science activists...scientist. I’ve worked tirelessly to get the publications, grant funding, and teaching experience that...

...pre-clinical life science work, economics) that published research is difficult to replicate.  Why is replicating...appreciate how few details are provided in most published papers. The precise timing, order, or every combination... rarely exist or are preserved as part of the publishing process and become lost if not archived. Remove...process of creating a time-stamped research plan in a public repository before conducting the experiment (note...registries do require that research plans become public eventually). It is typically done right before ... article is given “in-principle acceptance” to publish the final results regardless of the outcome of ...null findings that are notoriously difficult to publish.  With Registered Reports, the authors are able...

...Originally published Feb 14, 2017 and updated Jun 24, 2020. Epigenetic modifications are an additional...doi.org/10.1038/nbt.3199 Find plasmids from this publication at Addgene. Klann TS, Black JB, Chellappan M...doi.org/10.1038/nbt.3853 Find plasmids from this publication at Addgene.  Lei Y, Zhang X, Su J, Jeong M, ...10.1038/ncomms16026 Find plasmids from this publication at Addgene. Liu XS, Wu H, Ji X, Stelzer Y, Wu.../j.cell.2016.08.056 Find plasmids from this publication at Addgene. Maeder ML, Angstman JF, Richardson...doi.org/10.1038/nbt.2726 Find plasmids from this publication at Addgene. McDonald JI, Celik H, Rois LE, Fishberger.../10.1242/bio.019067 Find plasmids from this publication at Addgene. Mendenhall EM, Williamson KE, Reyon...

...lab while the publication page contains all the plasmids deposited from the same publication. Next up, we... available through Addgene. Publication: If the plasmid has been published, the paper in which it was ...through the depositing lab page, then onto the publication page, and finally on down to the individual plasmid...Great! Now, if I want to use this plasmid in a publication, how do I know who to acknowledge? Depositing...the bottom of the page that help you cite this publication in your own work: How to cite this plasmid: The...depositing lab whenever their plasmids are used in a publication. Addgene makes this easy by providing a template...of our plasmid pages, and provides a list of publications which have used the plasmid (see our blog post...

...to peer-reviewed journal publications, although many don’t necessarily publish in OA journals themselves...in annual R&D expenditures to support increased public access to the results of federally-funded research...world. 4)      The Annals of Internal Medicine published systematic reviews on an agent used to promote...an organization called Free the Data launched a public database to gather the results of Myriad's genetic... into account. 7)     Science’s John Bohannon published an article describing a sting operation in which...submitted a phony paper to a list of 304 open access publishers. By the time the magazine went to press, no less...gave preference to journal access granted via a public repository or authors’ own web sites. 9)      The...

...scientific publishing world and debate issues about how to improve the scientific publishing process. There...variety of careers and jobs. At Science, which is published by the American Association for the Advancement...as financially critical. Those who work on the publishing side of science have professional meetings, societies...access affects financial models in information publication. Take a look at the blog ‘Scholarly Kitchen’ ...’ for a glimpse into the world of scholarly publishing. And see for example the Eighth International Congress... Congress on Peer Review and Scientific Publication, where diverse approaches to peer review were discussed...Hines is a Senior Editor at Science magazine, published by the American Association for the Advancement...

...Co-Ordinating Centre for Public Engagement (NCCPE) provides on-site courses in public engagement for institutions...communication activities, e.g. tweet it when you publish a new blog post, and live tweet from conferences...tips on using social media as a scientist here. 2) Public engagement - Participate in science festivals and... and outreach If you are comfortable talking in public, volunteer at science festivals. Or engage with...performance. When your institute opens its doors to the public, for example at an anniversary or for Girls’ Day...for 10 busy weeks at media outlets like National Public Radio, WIRED, Scientific American or National Geographic...projects Lastly, if you'd like to directly involve the public in your research projects, you can set up a citizen...

...circular assemblies. While Golden Gate Assembly worked for all the assembly types, circular assemblies yielded...J., & Lohman, G. J. S. (2020). Enabling one-pot Golden Gate assemblies of unprecedented complexity using... an increase in the complexity. In 2018, they published a paper profiling four base overhang ligation ... researchers design high-fidelity Golden Gate Assemblies and overhang sets: NEBridge Ligase Fidelity Viewer...Figure 1: Workflow for high complexity Golden Gate Assemblies. Image courtesy of New England Biolabs.   ...the 52-piece reaction shows massively complex assemblies are possible, the two sets of data together indicate...

... One obvious way to do that is and has been to publish in journals with a high “impact factor,” a measure... citations for recent articles. Of course, a publication in Science or Nature is always nice, but in the... lesser-known journal as it is in a top-tier publication. Accessibility is surely also behind the increase...increase in citations for older publications. (As an interesting aside, Nature recently reported that the...in a solution.) But are citations and journal publications really the best way to measure impact? As that...and what it’s made of. Increasingly, journal publications alone aren’t enough. Scientists are sharing ...consider and quantify the impact of scientific publications. Ventures like Impact Story seek to find ways...

...2013 there have been 33 CRISPR software tools published and documented by OMICtools. It must be confusing... are altogether different. They are filled with blind anticipation. Their trust in the quality of the ...reviewing the contents of the piñata and the audience blindly receiving them; it results in highly similar content...; it is caused by not having the time to assess public response; and, it is the stage I think we are currently... CRISPR software submissions. With few prior publications to go on, a submitted manuscript could only ...The first of only three such databases, EENdb (published 1 Jan 2013, no longer available), is a simple ...sgRNA designs. The second database, CrisprGE (published 27 Jun 2015), is noteworthy for its broad scope...

...for Microbiology (ASM) Scientific Writing and Publishing Institute. Writing is the cornerstone of any ... them with the world. If one doesn’t write and publish in some way, then the data produced isn’t likely...simply writing down your data doesn’t guarantee a publication. There is an art to composing manuscripts and...and the ASM Scientific Writing and Publishing Institute (SWPI) is an extremely beneficial guide to becoming...field of microbiology with multiple high-profile publications. My classmates consisted of graduate students...paper will be generated. After you complete a publication quality figure, produce the figure, its legend...Resources on the Addgene Blog Dos & Don'ts When Publishing a Scientific Manuscript Writing Scientific Manuscripts...

...Multiple first or corresponding author publications (published, not in preparation) A history of successful...A project with sufficient preliminary data to establish your own lab and get an independent investigator...the beginning of Year 5. Now work backwards. To publish a submitted manuscript in a high-profile journal...The other items will follow. If you get more publications, you will be more competitive for fellowships...typically have 4-5 years to make discoveries and publish papers to position yourself for a job search. Therefore...the best possible outcome, e.g. where could you publish this and how important is the result? Is there... meet future collaborators and get access to unpublished information and reagents. Part of the reason ...

...isolation. It published a paper a year (or less) and limited the exchange of information to the public and to...to other academic labs. Being unable to publish or attend conferences makes it very challenging to compete...As a Master’s student, she had less pressure to publish and more freedom to attend conferences, but after...occasionally thinking about how we could bypass the establishment hiring system altogether. We had a lot of ideas... organisms, and GMOs have a bad reputation. The public is can be fearful of this technology and angry ...something new and beautiful, to engage with the public in a meaningful way about science, and to have ...

...best way to share these improvements? You could publish a paper that happens to include your improvements...manuscript to get reviewed and edited prior to publication, and this will delay the dissemination of your...your improved protocol. Many scientists, publishers, and small start-ups are turning to software apps to... makes it easy to annotate protocols, make them public for others to use, and add your own comments. Currently...online protocol repository hosted by the Nature Publishing Group. Like the previously mentioned sites, NPE...charge to scientists by allowing companies to pay publish their catalogs on the site. Like Labguru, Quartzy...Freedom: The Expansion of Data Sharing in Research Publications by Jim Woodgett        ...

...Originally published Nov 12, 2015 and last updated Aug 20, 2020. Cas9 can be used to modify any desired...doi.org/10.1038/nbt.3609 Find plasmids from this publication at Addgene. Kim E, Koo T, Park SW, Kim D, Kim...10.1038/ncomms14500 Find plasmids from this publication at Addgene. Kleinstiver BP, Prew MS, Tsai SQ...10.1038/nature14592 Find plasmids from this publication at Addgene. Kleinstiver BP, Tsai SQ, Prew MS...doi.org/10.1038/nbt.3620 Find plasmids from this publication at Addgene. Hu JH, Miller SM, Geurts MH, Tang...10.1038/nature14299 Find plasmids from this publication at Addgene. Walton RT, Christie KA, Whittaker.../j.cell.2015.09.038 Find plasmids from this publication at Addgene. Additional Resources on the Addgene...